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Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood
In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505318/ https://www.ncbi.nlm.nih.gov/pubmed/36144129 http://dx.doi.org/10.3390/mi13091506 |
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author | Hendrickson, Olga D. Zvereva, Elena A. Solopova, Olga N. Zherdev, Anatoly V. Sveshnikov, Peter G. Eremin, Sergei A. Dzantiev, Boris B. |
author_facet | Hendrickson, Olga D. Zvereva, Elena A. Solopova, Olga N. Zherdev, Anatoly V. Sveshnikov, Peter G. Eremin, Sergei A. Dzantiev, Boris B. |
author_sort | Hendrickson, Olga D. |
collection | PubMed |
description | In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal conditions, the instrumental detection limits/cutoffs for simultaneous detection of DA and OA were 1.2/100 and 0.1/2.5 ng/mL, respectively. The time of the assay was 18 min. The ICA was applied to test seawater and a large panel of seafood, including mussels, tiger shrimps, octopuses, whelks, crabs, and scallops. The proposed simple sample preparation method for seafood takes only 20 min. For seawater, a dilution by buffer was implemented. The assay recoveries varied from 80.8% to 124.5%. The competitive potential of the proposed technique as a tool to control natural water and seafood samples is determined by its simplicity, rapidity, and sensitivity. |
format | Online Article Text |
id | pubmed-9505318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95053182022-09-24 Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood Hendrickson, Olga D. Zvereva, Elena A. Solopova, Olga N. Zherdev, Anatoly V. Sveshnikov, Peter G. Eremin, Sergei A. Dzantiev, Boris B. Micromachines (Basel) Article In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal conditions, the instrumental detection limits/cutoffs for simultaneous detection of DA and OA were 1.2/100 and 0.1/2.5 ng/mL, respectively. The time of the assay was 18 min. The ICA was applied to test seawater and a large panel of seafood, including mussels, tiger shrimps, octopuses, whelks, crabs, and scallops. The proposed simple sample preparation method for seafood takes only 20 min. For seawater, a dilution by buffer was implemented. The assay recoveries varied from 80.8% to 124.5%. The competitive potential of the proposed technique as a tool to control natural water and seafood samples is determined by its simplicity, rapidity, and sensitivity. MDPI 2022-09-10 /pmc/articles/PMC9505318/ /pubmed/36144129 http://dx.doi.org/10.3390/mi13091506 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hendrickson, Olga D. Zvereva, Elena A. Solopova, Olga N. Zherdev, Anatoly V. Sveshnikov, Peter G. Eremin, Sergei A. Dzantiev, Boris B. Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
title | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
title_full | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
title_fullStr | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
title_full_unstemmed | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
title_short | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
title_sort | double immunochromatographic test system for sensitive detection of phycotoxins domoic acid and okadaic acid in seawater and seafood |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505318/ https://www.ncbi.nlm.nih.gov/pubmed/36144129 http://dx.doi.org/10.3390/mi13091506 |
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