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Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats

Adversity is particularly pernicious in early life, increasing the likelihood of developing psychiatric disorders in adulthood. Juvenile and adult rats exposed to social isolation show differences in anxiety-like behaviors and significant changes in dopamine (DA) neurotransmission in the nucleus acc...

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Autores principales: Zegers-Delgado, Juan, Aguilera-Soza, Alejandro, Calderón, Florencia, Davidson, Harley, Verbel-Vergara, Daniel, Yarur, Hector E., Novoa, Javier, Blanlot, Camila, Bastias, Cristian P., Andrés, María Estela, Gysling, Katia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505341/
https://www.ncbi.nlm.nih.gov/pubmed/36142716
http://dx.doi.org/10.3390/ijms231810800
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author Zegers-Delgado, Juan
Aguilera-Soza, Alejandro
Calderón, Florencia
Davidson, Harley
Verbel-Vergara, Daniel
Yarur, Hector E.
Novoa, Javier
Blanlot, Camila
Bastias, Cristian P.
Andrés, María Estela
Gysling, Katia
author_facet Zegers-Delgado, Juan
Aguilera-Soza, Alejandro
Calderón, Florencia
Davidson, Harley
Verbel-Vergara, Daniel
Yarur, Hector E.
Novoa, Javier
Blanlot, Camila
Bastias, Cristian P.
Andrés, María Estela
Gysling, Katia
author_sort Zegers-Delgado, Juan
collection PubMed
description Adversity is particularly pernicious in early life, increasing the likelihood of developing psychiatric disorders in adulthood. Juvenile and adult rats exposed to social isolation show differences in anxiety-like behaviors and significant changes in dopamine (DA) neurotransmission in the nucleus accumbens (NAc). Brain response to stress is partly mediated by the corticotropin-releasing factor (CRF) system, composed of CRF and its two main receptors, CRF-R1 and CRF-R2. In the NAc shell of adult rats, CRF induces anxiety-like behavior and changes local DA balance. However, the role of CRF receptors in the control of neurotransmission in the NAc is not fully understood, nor is it known whether there are differences between life stages. Our previous data showed that infusion of a CRF-R1 antagonist into the NAc of juvenile rats increased DA levels in response to a depolarizing stimulus and decreased basal glutamate levels. To extend this analysis, we now evaluated the effect of a CRF-R1 antagonist infusion in the NAc of adult rats. Here, we describe that the opposite occurred in the NAc of adult compared to juvenile rats. Infusion of a CRF-R1 antagonist decreased DA and increased glutamate levels in response to a depolarizing stimulus. Furthermore, basal levels of DA, glutamate, and γ-Aminobutyric acid (GABA) were similar in juvenile animals compared to adults. CRF-R1 protein levels and localization were not different in juvenile compared to adult rats. Interestingly, we observed differences in the signaling pathways of CRF-R1 in the NAc of juveniles compared to adult rats. We propose that the function of CRF-R1 receptors is differentially modulated in the NAc according to life stage.
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spelling pubmed-95053412022-09-24 Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats Zegers-Delgado, Juan Aguilera-Soza, Alejandro Calderón, Florencia Davidson, Harley Verbel-Vergara, Daniel Yarur, Hector E. Novoa, Javier Blanlot, Camila Bastias, Cristian P. Andrés, María Estela Gysling, Katia Int J Mol Sci Article Adversity is particularly pernicious in early life, increasing the likelihood of developing psychiatric disorders in adulthood. Juvenile and adult rats exposed to social isolation show differences in anxiety-like behaviors and significant changes in dopamine (DA) neurotransmission in the nucleus accumbens (NAc). Brain response to stress is partly mediated by the corticotropin-releasing factor (CRF) system, composed of CRF and its two main receptors, CRF-R1 and CRF-R2. In the NAc shell of adult rats, CRF induces anxiety-like behavior and changes local DA balance. However, the role of CRF receptors in the control of neurotransmission in the NAc is not fully understood, nor is it known whether there are differences between life stages. Our previous data showed that infusion of a CRF-R1 antagonist into the NAc of juvenile rats increased DA levels in response to a depolarizing stimulus and decreased basal glutamate levels. To extend this analysis, we now evaluated the effect of a CRF-R1 antagonist infusion in the NAc of adult rats. Here, we describe that the opposite occurred in the NAc of adult compared to juvenile rats. Infusion of a CRF-R1 antagonist decreased DA and increased glutamate levels in response to a depolarizing stimulus. Furthermore, basal levels of DA, glutamate, and γ-Aminobutyric acid (GABA) were similar in juvenile animals compared to adults. CRF-R1 protein levels and localization were not different in juvenile compared to adult rats. Interestingly, we observed differences in the signaling pathways of CRF-R1 in the NAc of juveniles compared to adult rats. We propose that the function of CRF-R1 receptors is differentially modulated in the NAc according to life stage. MDPI 2022-09-16 /pmc/articles/PMC9505341/ /pubmed/36142716 http://dx.doi.org/10.3390/ijms231810800 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zegers-Delgado, Juan
Aguilera-Soza, Alejandro
Calderón, Florencia
Davidson, Harley
Verbel-Vergara, Daniel
Yarur, Hector E.
Novoa, Javier
Blanlot, Camila
Bastias, Cristian P.
Andrés, María Estela
Gysling, Katia
Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats
title Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats
title_full Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats
title_fullStr Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats
title_full_unstemmed Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats
title_short Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats
title_sort type 1 corticotropin-releasing factor receptor differentially modulates neurotransmitter levels in the nucleus accumbens of juvenile versus adult rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505341/
https://www.ncbi.nlm.nih.gov/pubmed/36142716
http://dx.doi.org/10.3390/ijms231810800
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