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Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae

Microplastics, small pieces of plastic derived from polystyrene, have recently become an ecological hazard due to their toxicity and widespread occurrence in aquatic ecosystems. In this study, we exposed zebrafish larvae to two types of fluorescent polystyrene nanoparticles (PS-NPs) to identify thei...

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Autores principales: Hwang, Kyu-Seok, Son, Yuji, Kim, Seong Soon, Shin, Dae-Seop, Lim, So Hee, Yang, Jung Yoon, Jeong, Ha Neul, Lee, Byung Hoi, Bae, Myung Ae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505408/
https://www.ncbi.nlm.nih.gov/pubmed/36142594
http://dx.doi.org/10.3390/ijms231810682
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author Hwang, Kyu-Seok
Son, Yuji
Kim, Seong Soon
Shin, Dae-Seop
Lim, So Hee
Yang, Jung Yoon
Jeong, Ha Neul
Lee, Byung Hoi
Bae, Myung Ae
author_facet Hwang, Kyu-Seok
Son, Yuji
Kim, Seong Soon
Shin, Dae-Seop
Lim, So Hee
Yang, Jung Yoon
Jeong, Ha Neul
Lee, Byung Hoi
Bae, Myung Ae
author_sort Hwang, Kyu-Seok
collection PubMed
description Microplastics, small pieces of plastic derived from polystyrene, have recently become an ecological hazard due to their toxicity and widespread occurrence in aquatic ecosystems. In this study, we exposed zebrafish larvae to two types of fluorescent polystyrene nanoparticles (PS-NPs) to identify their size-dependent effects. PS-NPs of 50 nm, unlike 100 nm PS-NPs, were found to circulate in the blood vessels and accumulate in the brains of zebrafish larvae. Behavioral and electroencephalogram (EEG) analysis showed that 50 nm PS-NPs induce abnormal behavioral patterns and changes in EEG power spectral densities in zebrafish larvae. In addition, the quantification of endogenous neurochemicals in zebrafish larvae showed that 50 nm PS-NPs disturb dopaminergic metabolites, whereas 100 nm PS-NPs do not. Finally, we assessed the effect of PS-NPs on the permeability of the blood–brain barrier (BBB) using a microfluidic system. The results revealed that 50 nm PS-NPs have high BBB penetration compared with 100 nm PS-NPs. Taken together, we concluded that small nanoparticles disturb the nervous system, especially dopaminergic metabolites.
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spelling pubmed-95054082022-09-24 Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae Hwang, Kyu-Seok Son, Yuji Kim, Seong Soon Shin, Dae-Seop Lim, So Hee Yang, Jung Yoon Jeong, Ha Neul Lee, Byung Hoi Bae, Myung Ae Int J Mol Sci Article Microplastics, small pieces of plastic derived from polystyrene, have recently become an ecological hazard due to their toxicity and widespread occurrence in aquatic ecosystems. In this study, we exposed zebrafish larvae to two types of fluorescent polystyrene nanoparticles (PS-NPs) to identify their size-dependent effects. PS-NPs of 50 nm, unlike 100 nm PS-NPs, were found to circulate in the blood vessels and accumulate in the brains of zebrafish larvae. Behavioral and electroencephalogram (EEG) analysis showed that 50 nm PS-NPs induce abnormal behavioral patterns and changes in EEG power spectral densities in zebrafish larvae. In addition, the quantification of endogenous neurochemicals in zebrafish larvae showed that 50 nm PS-NPs disturb dopaminergic metabolites, whereas 100 nm PS-NPs do not. Finally, we assessed the effect of PS-NPs on the permeability of the blood–brain barrier (BBB) using a microfluidic system. The results revealed that 50 nm PS-NPs have high BBB penetration compared with 100 nm PS-NPs. Taken together, we concluded that small nanoparticles disturb the nervous system, especially dopaminergic metabolites. MDPI 2022-09-14 /pmc/articles/PMC9505408/ /pubmed/36142594 http://dx.doi.org/10.3390/ijms231810682 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hwang, Kyu-Seok
Son, Yuji
Kim, Seong Soon
Shin, Dae-Seop
Lim, So Hee
Yang, Jung Yoon
Jeong, Ha Neul
Lee, Byung Hoi
Bae, Myung Ae
Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae
title Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae
title_full Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae
title_fullStr Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae
title_full_unstemmed Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae
title_short Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae
title_sort size-dependent effects of polystyrene nanoparticles (ps-nps) on behaviors and endogenous neurochemicals in zebrafish larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505408/
https://www.ncbi.nlm.nih.gov/pubmed/36142594
http://dx.doi.org/10.3390/ijms231810682
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