Cargando…
Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis
Background: Tissue regeneration is a complex process that allows wounds to heal. Many options are currently available to help human skin repair and to reduce the recurrence of hernias. The aim of this study is to analyze the best decellularization protocol for allogenic human dermal tissues. Methods...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505465/ https://www.ncbi.nlm.nih.gov/pubmed/36143199 http://dx.doi.org/10.3390/jpm12091411 |
_version_ | 1784796479383142400 |
---|---|
author | Altomare, Michele Ferrario, Luca Benuzzi, Laura Tosca, Marta Cecilia Gipponi, Marta Khodri, Imam Sesana, Giovanni Cimbanassi, Stefania Cioffi, Stefano Piero Bernardo Spota, Andrea Bini, Roberto Chiara, Osvaldo Mingoli, Andrea |
author_facet | Altomare, Michele Ferrario, Luca Benuzzi, Laura Tosca, Marta Cecilia Gipponi, Marta Khodri, Imam Sesana, Giovanni Cimbanassi, Stefania Cioffi, Stefano Piero Bernardo Spota, Andrea Bini, Roberto Chiara, Osvaldo Mingoli, Andrea |
author_sort | Altomare, Michele |
collection | PubMed |
description | Background: Tissue regeneration is a complex process that allows wounds to heal. Many options are currently available to help human skin repair and to reduce the recurrence of hernias. The aim of this study is to analyze the best decellularization protocol for allogenic human dermal tissues. Methods: Dermal flaps from donors were used and compared with a control group. Each flap was subjected to seven different decellularization protocols and washed with a sequence of five solutions. The samples were then subjected to four control tests (such as Nile Red), and long-term contacts were analyzed to assess whether the decellularized dermis samples could support the growth of human fibroblasts. Results: All the samples had an average residual viability of 60%. Except for one sample, the decellularization treatments were able to reduce cell viability significantly. The Nile Red test showed a significant reduction in phospholipid content (mean 90%, p-value < 0.05) in all treatments. The cell growth increased in a linear manner. As described in the literature, sodium-dodecyl-sulfate (SDS) caused an interference between the test and the detergent. Conclusions: This paper shows the first step to finding the best decellularization protocol for allografting human dermal tissues. Further biocompatibility tests and DNA quantification are necessary. |
format | Online Article Text |
id | pubmed-9505465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95054652022-09-24 Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis Altomare, Michele Ferrario, Luca Benuzzi, Laura Tosca, Marta Cecilia Gipponi, Marta Khodri, Imam Sesana, Giovanni Cimbanassi, Stefania Cioffi, Stefano Piero Bernardo Spota, Andrea Bini, Roberto Chiara, Osvaldo Mingoli, Andrea J Pers Med Article Background: Tissue regeneration is a complex process that allows wounds to heal. Many options are currently available to help human skin repair and to reduce the recurrence of hernias. The aim of this study is to analyze the best decellularization protocol for allogenic human dermal tissues. Methods: Dermal flaps from donors were used and compared with a control group. Each flap was subjected to seven different decellularization protocols and washed with a sequence of five solutions. The samples were then subjected to four control tests (such as Nile Red), and long-term contacts were analyzed to assess whether the decellularized dermis samples could support the growth of human fibroblasts. Results: All the samples had an average residual viability of 60%. Except for one sample, the decellularization treatments were able to reduce cell viability significantly. The Nile Red test showed a significant reduction in phospholipid content (mean 90%, p-value < 0.05) in all treatments. The cell growth increased in a linear manner. As described in the literature, sodium-dodecyl-sulfate (SDS) caused an interference between the test and the detergent. Conclusions: This paper shows the first step to finding the best decellularization protocol for allografting human dermal tissues. Further biocompatibility tests and DNA quantification are necessary. MDPI 2022-08-30 /pmc/articles/PMC9505465/ /pubmed/36143199 http://dx.doi.org/10.3390/jpm12091411 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Altomare, Michele Ferrario, Luca Benuzzi, Laura Tosca, Marta Cecilia Gipponi, Marta Khodri, Imam Sesana, Giovanni Cimbanassi, Stefania Cioffi, Stefano Piero Bernardo Spota, Andrea Bini, Roberto Chiara, Osvaldo Mingoli, Andrea Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis |
title | Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis |
title_full | Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis |
title_fullStr | Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis |
title_full_unstemmed | Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis |
title_short | Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis |
title_sort | regenerative medicine to improve outcomes of ventral hernia reconstruction (repair study) phase 1: find the best decellularization protocol for the human dermis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505465/ https://www.ncbi.nlm.nih.gov/pubmed/36143199 http://dx.doi.org/10.3390/jpm12091411 |
work_keys_str_mv | AT altomaremichele regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT ferrarioluca regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT benuzzilaura regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT toscamartacecilia regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT gipponimarta regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT khodriimam regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT sesanagiovanni regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT cimbanassistefania regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT cioffistefanopierobernardo regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT spotaandrea regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT biniroberto regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT chiaraosvaldo regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis AT mingoliandrea regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis |