Cargando…

Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis

Background: Tissue regeneration is a complex process that allows wounds to heal. Many options are currently available to help human skin repair and to reduce the recurrence of hernias. The aim of this study is to analyze the best decellularization protocol for allogenic human dermal tissues. Methods...

Descripción completa

Detalles Bibliográficos
Autores principales: Altomare, Michele, Ferrario, Luca, Benuzzi, Laura, Tosca, Marta Cecilia, Gipponi, Marta, Khodri, Imam, Sesana, Giovanni, Cimbanassi, Stefania, Cioffi, Stefano Piero Bernardo, Spota, Andrea, Bini, Roberto, Chiara, Osvaldo, Mingoli, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505465/
https://www.ncbi.nlm.nih.gov/pubmed/36143199
http://dx.doi.org/10.3390/jpm12091411
_version_ 1784796479383142400
author Altomare, Michele
Ferrario, Luca
Benuzzi, Laura
Tosca, Marta Cecilia
Gipponi, Marta
Khodri, Imam
Sesana, Giovanni
Cimbanassi, Stefania
Cioffi, Stefano Piero Bernardo
Spota, Andrea
Bini, Roberto
Chiara, Osvaldo
Mingoli, Andrea
author_facet Altomare, Michele
Ferrario, Luca
Benuzzi, Laura
Tosca, Marta Cecilia
Gipponi, Marta
Khodri, Imam
Sesana, Giovanni
Cimbanassi, Stefania
Cioffi, Stefano Piero Bernardo
Spota, Andrea
Bini, Roberto
Chiara, Osvaldo
Mingoli, Andrea
author_sort Altomare, Michele
collection PubMed
description Background: Tissue regeneration is a complex process that allows wounds to heal. Many options are currently available to help human skin repair and to reduce the recurrence of hernias. The aim of this study is to analyze the best decellularization protocol for allogenic human dermal tissues. Methods: Dermal flaps from donors were used and compared with a control group. Each flap was subjected to seven different decellularization protocols and washed with a sequence of five solutions. The samples were then subjected to four control tests (such as Nile Red), and long-term contacts were analyzed to assess whether the decellularized dermis samples could support the growth of human fibroblasts. Results: All the samples had an average residual viability of 60%. Except for one sample, the decellularization treatments were able to reduce cell viability significantly. The Nile Red test showed a significant reduction in phospholipid content (mean 90%, p-value < 0.05) in all treatments. The cell growth increased in a linear manner. As described in the literature, sodium-dodecyl-sulfate (SDS) caused an interference between the test and the detergent. Conclusions: This paper shows the first step to finding the best decellularization protocol for allografting human dermal tissues. Further biocompatibility tests and DNA quantification are necessary.
format Online
Article
Text
id pubmed-9505465
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95054652022-09-24 Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis Altomare, Michele Ferrario, Luca Benuzzi, Laura Tosca, Marta Cecilia Gipponi, Marta Khodri, Imam Sesana, Giovanni Cimbanassi, Stefania Cioffi, Stefano Piero Bernardo Spota, Andrea Bini, Roberto Chiara, Osvaldo Mingoli, Andrea J Pers Med Article Background: Tissue regeneration is a complex process that allows wounds to heal. Many options are currently available to help human skin repair and to reduce the recurrence of hernias. The aim of this study is to analyze the best decellularization protocol for allogenic human dermal tissues. Methods: Dermal flaps from donors were used and compared with a control group. Each flap was subjected to seven different decellularization protocols and washed with a sequence of five solutions. The samples were then subjected to four control tests (such as Nile Red), and long-term contacts were analyzed to assess whether the decellularized dermis samples could support the growth of human fibroblasts. Results: All the samples had an average residual viability of 60%. Except for one sample, the decellularization treatments were able to reduce cell viability significantly. The Nile Red test showed a significant reduction in phospholipid content (mean 90%, p-value < 0.05) in all treatments. The cell growth increased in a linear manner. As described in the literature, sodium-dodecyl-sulfate (SDS) caused an interference between the test and the detergent. Conclusions: This paper shows the first step to finding the best decellularization protocol for allografting human dermal tissues. Further biocompatibility tests and DNA quantification are necessary. MDPI 2022-08-30 /pmc/articles/PMC9505465/ /pubmed/36143199 http://dx.doi.org/10.3390/jpm12091411 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Altomare, Michele
Ferrario, Luca
Benuzzi, Laura
Tosca, Marta Cecilia
Gipponi, Marta
Khodri, Imam
Sesana, Giovanni
Cimbanassi, Stefania
Cioffi, Stefano Piero Bernardo
Spota, Andrea
Bini, Roberto
Chiara, Osvaldo
Mingoli, Andrea
Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis
title Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis
title_full Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis
title_fullStr Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis
title_full_unstemmed Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis
title_short Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis
title_sort regenerative medicine to improve outcomes of ventral hernia reconstruction (repair study) phase 1: find the best decellularization protocol for the human dermis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505465/
https://www.ncbi.nlm.nih.gov/pubmed/36143199
http://dx.doi.org/10.3390/jpm12091411
work_keys_str_mv AT altomaremichele regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT ferrarioluca regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT benuzzilaura regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT toscamartacecilia regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT gipponimarta regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT khodriimam regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT sesanagiovanni regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT cimbanassistefania regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT cioffistefanopierobernardo regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT spotaandrea regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT biniroberto regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT chiaraosvaldo regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis
AT mingoliandrea regenerativemedicinetoimproveoutcomesofventralherniareconstructionrepairstudyphase1findthebestdecellularizationprotocolforthehumandermis