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Evaluation of 3D Printability and Biocompatibility of Microfluidic Resin for Fabrication of Solid Microneedles

In this study, we have employed Digital Light Processing (DLP) printing technology for the fabrication of solid microneedle (MN) arrays. Several arrays with various geometries, such as cones, three-sided pyramids and four-sided pyramids, with different height to aspect ratios of 1:1, 2:1 and 3:1, we...

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Autores principales: Tabriz, Atabak Ghanizadeh, Viegas, Beatriz, Okereke, Michael, Uddin, Md Jasim, Lopez, Elena Arribas, Zand, Nazanin, Ranatunga, Medhavi, Getti, Giulia, Douroumis, Dennis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505489/
https://www.ncbi.nlm.nih.gov/pubmed/36143991
http://dx.doi.org/10.3390/mi13091368
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author Tabriz, Atabak Ghanizadeh
Viegas, Beatriz
Okereke, Michael
Uddin, Md Jasim
Lopez, Elena Arribas
Zand, Nazanin
Ranatunga, Medhavi
Getti, Giulia
Douroumis, Dennis
author_facet Tabriz, Atabak Ghanizadeh
Viegas, Beatriz
Okereke, Michael
Uddin, Md Jasim
Lopez, Elena Arribas
Zand, Nazanin
Ranatunga, Medhavi
Getti, Giulia
Douroumis, Dennis
author_sort Tabriz, Atabak Ghanizadeh
collection PubMed
description In this study, we have employed Digital Light Processing (DLP) printing technology for the fabrication of solid microneedle (MN) arrays. Several arrays with various geometries, such as cones, three-sided pyramids and four-sided pyramids, with different height to aspect ratios of 1:1, 2:1 and 3:1, were printed. Post-processing curing optimizations showed that optimal mechanical properties of the photocurable resin were obtained at 40 °C and 60 min. Ex vivo skin studies showed that piercing forces, penetration depth and penetration width were affected by the MN geometry and height to aspect ratio. Cone-shaped MNs required lower applied forces to penetrate skin and showed higher penetration depth with increasing height to aspect ratio, followed by three-sided and four-sided printed arrays. Cytotoxicity studies presented 84% cell viability of human fibroblasts after 2.5 h, suggesting the very good biocompatibility of the photocurable resin. Overall, DLP demonstrated excellent printing capacity and high resolution for a variety of MN designs.
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spelling pubmed-95054892022-09-24 Evaluation of 3D Printability and Biocompatibility of Microfluidic Resin for Fabrication of Solid Microneedles Tabriz, Atabak Ghanizadeh Viegas, Beatriz Okereke, Michael Uddin, Md Jasim Lopez, Elena Arribas Zand, Nazanin Ranatunga, Medhavi Getti, Giulia Douroumis, Dennis Micromachines (Basel) Article In this study, we have employed Digital Light Processing (DLP) printing technology for the fabrication of solid microneedle (MN) arrays. Several arrays with various geometries, such as cones, three-sided pyramids and four-sided pyramids, with different height to aspect ratios of 1:1, 2:1 and 3:1, were printed. Post-processing curing optimizations showed that optimal mechanical properties of the photocurable resin were obtained at 40 °C and 60 min. Ex vivo skin studies showed that piercing forces, penetration depth and penetration width were affected by the MN geometry and height to aspect ratio. Cone-shaped MNs required lower applied forces to penetrate skin and showed higher penetration depth with increasing height to aspect ratio, followed by three-sided and four-sided printed arrays. Cytotoxicity studies presented 84% cell viability of human fibroblasts after 2.5 h, suggesting the very good biocompatibility of the photocurable resin. Overall, DLP demonstrated excellent printing capacity and high resolution for a variety of MN designs. MDPI 2022-08-23 /pmc/articles/PMC9505489/ /pubmed/36143991 http://dx.doi.org/10.3390/mi13091368 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tabriz, Atabak Ghanizadeh
Viegas, Beatriz
Okereke, Michael
Uddin, Md Jasim
Lopez, Elena Arribas
Zand, Nazanin
Ranatunga, Medhavi
Getti, Giulia
Douroumis, Dennis
Evaluation of 3D Printability and Biocompatibility of Microfluidic Resin for Fabrication of Solid Microneedles
title Evaluation of 3D Printability and Biocompatibility of Microfluidic Resin for Fabrication of Solid Microneedles
title_full Evaluation of 3D Printability and Biocompatibility of Microfluidic Resin for Fabrication of Solid Microneedles
title_fullStr Evaluation of 3D Printability and Biocompatibility of Microfluidic Resin for Fabrication of Solid Microneedles
title_full_unstemmed Evaluation of 3D Printability and Biocompatibility of Microfluidic Resin for Fabrication of Solid Microneedles
title_short Evaluation of 3D Printability and Biocompatibility of Microfluidic Resin for Fabrication of Solid Microneedles
title_sort evaluation of 3d printability and biocompatibility of microfluidic resin for fabrication of solid microneedles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505489/
https://www.ncbi.nlm.nih.gov/pubmed/36143991
http://dx.doi.org/10.3390/mi13091368
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