Cargando…

Anti-HBc IgG Responses Occurring at the Early Phase of Infection Correlate Negatively with HBV Replication in a Mouse Model

Anti-HBc IgG is usually recognized as a diagnostic marker of hepatitis B, while the functional role anti-HBc IgG in HBV infection has not been fully elucidated. In this study, we firstly investigated the relationship between the anti-HBc IgG responses and the replication of HBV using AAV8-1.3HBV inf...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xuyang, Zhang, Yumeng, Ben, Yinyin, Qiu, Chao, Wu, Jing, Zhang, Wenhong, Wan, Yanmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505635/
https://www.ncbi.nlm.nih.gov/pubmed/36146817
http://dx.doi.org/10.3390/v14092011
_version_ 1784796522023485440
author Wang, Xuyang
Zhang, Yumeng
Ben, Yinyin
Qiu, Chao
Wu, Jing
Zhang, Wenhong
Wan, Yanmin
author_facet Wang, Xuyang
Zhang, Yumeng
Ben, Yinyin
Qiu, Chao
Wu, Jing
Zhang, Wenhong
Wan, Yanmin
author_sort Wang, Xuyang
collection PubMed
description Anti-HBc IgG is usually recognized as a diagnostic marker of hepatitis B, while the functional role anti-HBc IgG in HBV infection has not been fully elucidated. In this study, we firstly investigated the relationship between the anti-HBc IgG responses and the replication of HBV using AAV8-1.3HBV infected C57BL/6N mice. Our data showed that the anti-HBc IgG responses at the early phase of infection correlated negatively with the concentrations of circulating HBsAg and HBV DNA at both the early and chronic phases of infection. This observation was confirmed by an independent experiment using AAV8-1.3HBV infected C57BL/6J mice. Furthermore, to comprehend the potential causal relationship between the anti-HBc IgG responses and HBV infection, mice were treated with an anti-HBc monoclonal antibody at three days post AAV8-1.3HBV infection. Our data showed that the anti-HBc mAb significantly suppressed the fold increase of circulating HBsAg level, and the protective effect was not affected by NK cell depletion. Collectively, our study demonstrated that anti-HBc antibodies occurring at the early phase of HBV infection may contribute to the constraint of the virus replication, which might be developed as an immunotherapy for hepatitis B.
format Online
Article
Text
id pubmed-9505635
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95056352022-09-24 Anti-HBc IgG Responses Occurring at the Early Phase of Infection Correlate Negatively with HBV Replication in a Mouse Model Wang, Xuyang Zhang, Yumeng Ben, Yinyin Qiu, Chao Wu, Jing Zhang, Wenhong Wan, Yanmin Viruses Article Anti-HBc IgG is usually recognized as a diagnostic marker of hepatitis B, while the functional role anti-HBc IgG in HBV infection has not been fully elucidated. In this study, we firstly investigated the relationship between the anti-HBc IgG responses and the replication of HBV using AAV8-1.3HBV infected C57BL/6N mice. Our data showed that the anti-HBc IgG responses at the early phase of infection correlated negatively with the concentrations of circulating HBsAg and HBV DNA at both the early and chronic phases of infection. This observation was confirmed by an independent experiment using AAV8-1.3HBV infected C57BL/6J mice. Furthermore, to comprehend the potential causal relationship between the anti-HBc IgG responses and HBV infection, mice were treated with an anti-HBc monoclonal antibody at three days post AAV8-1.3HBV infection. Our data showed that the anti-HBc mAb significantly suppressed the fold increase of circulating HBsAg level, and the protective effect was not affected by NK cell depletion. Collectively, our study demonstrated that anti-HBc antibodies occurring at the early phase of HBV infection may contribute to the constraint of the virus replication, which might be developed as an immunotherapy for hepatitis B. MDPI 2022-09-11 /pmc/articles/PMC9505635/ /pubmed/36146817 http://dx.doi.org/10.3390/v14092011 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xuyang
Zhang, Yumeng
Ben, Yinyin
Qiu, Chao
Wu, Jing
Zhang, Wenhong
Wan, Yanmin
Anti-HBc IgG Responses Occurring at the Early Phase of Infection Correlate Negatively with HBV Replication in a Mouse Model
title Anti-HBc IgG Responses Occurring at the Early Phase of Infection Correlate Negatively with HBV Replication in a Mouse Model
title_full Anti-HBc IgG Responses Occurring at the Early Phase of Infection Correlate Negatively with HBV Replication in a Mouse Model
title_fullStr Anti-HBc IgG Responses Occurring at the Early Phase of Infection Correlate Negatively with HBV Replication in a Mouse Model
title_full_unstemmed Anti-HBc IgG Responses Occurring at the Early Phase of Infection Correlate Negatively with HBV Replication in a Mouse Model
title_short Anti-HBc IgG Responses Occurring at the Early Phase of Infection Correlate Negatively with HBV Replication in a Mouse Model
title_sort anti-hbc igg responses occurring at the early phase of infection correlate negatively with hbv replication in a mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505635/
https://www.ncbi.nlm.nih.gov/pubmed/36146817
http://dx.doi.org/10.3390/v14092011
work_keys_str_mv AT wangxuyang antihbciggresponsesoccurringattheearlyphaseofinfectioncorrelatenegativelywithhbvreplicationinamousemodel
AT zhangyumeng antihbciggresponsesoccurringattheearlyphaseofinfectioncorrelatenegativelywithhbvreplicationinamousemodel
AT benyinyin antihbciggresponsesoccurringattheearlyphaseofinfectioncorrelatenegativelywithhbvreplicationinamousemodel
AT qiuchao antihbciggresponsesoccurringattheearlyphaseofinfectioncorrelatenegativelywithhbvreplicationinamousemodel
AT wujing antihbciggresponsesoccurringattheearlyphaseofinfectioncorrelatenegativelywithhbvreplicationinamousemodel
AT zhangwenhong antihbciggresponsesoccurringattheearlyphaseofinfectioncorrelatenegativelywithhbvreplicationinamousemodel
AT wanyanmin antihbciggresponsesoccurringattheearlyphaseofinfectioncorrelatenegativelywithhbvreplicationinamousemodel