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The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review

Introduction: The regeneration of dental pulp remains a challenge. Although several treatment modalities have been proposed to promote pulpal regeneration, these treatments have several drawbacks. More recently, decellularized dental pulp extracellular matrix (DP-ECM) has been proposed to regenerate...

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Autores principales: Adanir, Necdet, Khurshid, Zohaib, Ratnayake, Jithendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505725/
https://www.ncbi.nlm.nih.gov/pubmed/36143698
http://dx.doi.org/10.3390/ma15186386
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author Adanir, Necdet
Khurshid, Zohaib
Ratnayake, Jithendra
author_facet Adanir, Necdet
Khurshid, Zohaib
Ratnayake, Jithendra
author_sort Adanir, Necdet
collection PubMed
description Introduction: The regeneration of dental pulp remains a challenge. Although several treatment modalities have been proposed to promote pulpal regeneration, these treatments have several drawbacks. More recently, decellularized dental pulp extracellular matrix (DP-ECM) has been proposed to regenerate dental pulp. However, to date, no systematic review has summarized the overall outcome and assessed the available literature focusing on the endodontic use of DP-ECM. The aim of this systematic review is to critically appraise the literature, summarize the overall outcomes, and provide clinical recommendations about DP-ECM. Methodology: Following the Participants Intervention Control and Outcomes (PICO) principle, a focused question was constructed before conducting a search of the literature and of electronic research databases and registers. The focused question was: ‘Compared to controls, does decellularized dental pulp extracellular matrix (DP-ECM) stimulate the regeneration of dental pulp cells and tissue?’ Quality assessment of the studies was carried out using Guidelines for Reporting Pre-Clinical in Vitro Studies on Dental Materials and ARRIVE guidelines. Results: 12 studies were included in this review. Data from five in vitro experiments and eight in vivo experiments were extracted and the quality of the experiments was assessed. In majority of the studies, DP-ECM appeared to have stimulated pulpal regeneration. However, several sources of bias and methodological deficiencies were found during the quality assessment. Conclusion: Within the limitations of this review and the included studies, it may be concluded that there is insufficient evidence to deduce the overall efficacy of DP-ECM for pulpal regeneration. More research, clinical and pre-clinical, is required for more conclusive evidence.
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spelling pubmed-95057252022-09-24 The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review Adanir, Necdet Khurshid, Zohaib Ratnayake, Jithendra Materials (Basel) Systematic Review Introduction: The regeneration of dental pulp remains a challenge. Although several treatment modalities have been proposed to promote pulpal regeneration, these treatments have several drawbacks. More recently, decellularized dental pulp extracellular matrix (DP-ECM) has been proposed to regenerate dental pulp. However, to date, no systematic review has summarized the overall outcome and assessed the available literature focusing on the endodontic use of DP-ECM. The aim of this systematic review is to critically appraise the literature, summarize the overall outcomes, and provide clinical recommendations about DP-ECM. Methodology: Following the Participants Intervention Control and Outcomes (PICO) principle, a focused question was constructed before conducting a search of the literature and of electronic research databases and registers. The focused question was: ‘Compared to controls, does decellularized dental pulp extracellular matrix (DP-ECM) stimulate the regeneration of dental pulp cells and tissue?’ Quality assessment of the studies was carried out using Guidelines for Reporting Pre-Clinical in Vitro Studies on Dental Materials and ARRIVE guidelines. Results: 12 studies were included in this review. Data from five in vitro experiments and eight in vivo experiments were extracted and the quality of the experiments was assessed. In majority of the studies, DP-ECM appeared to have stimulated pulpal regeneration. However, several sources of bias and methodological deficiencies were found during the quality assessment. Conclusion: Within the limitations of this review and the included studies, it may be concluded that there is insufficient evidence to deduce the overall efficacy of DP-ECM for pulpal regeneration. More research, clinical and pre-clinical, is required for more conclusive evidence. MDPI 2022-09-14 /pmc/articles/PMC9505725/ /pubmed/36143698 http://dx.doi.org/10.3390/ma15186386 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Systematic Review
Adanir, Necdet
Khurshid, Zohaib
Ratnayake, Jithendra
The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review
title The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review
title_full The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review
title_fullStr The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review
title_full_unstemmed The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review
title_short The Regenerative Potential of Decellularized Dental Pulp Extracellular Matrix: A Systematic Review
title_sort regenerative potential of decellularized dental pulp extracellular matrix: a systematic review
topic Systematic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505725/
https://www.ncbi.nlm.nih.gov/pubmed/36143698
http://dx.doi.org/10.3390/ma15186386
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