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Serological and Molecular Survey for Dengue Virus Infection in Suspected Febrile Patients in Selected Local Government Areas in Adamawa State, Nigeria

Dengue is a disease caused by the dengue virus that is primarily transmitted by Aedes aegypti mosquitoes. Currently, the disease poses a threat to public health, with about 390 million people reported to be infected annually across the endemic regions of the world. In Nigeria, the disease is under-r...

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Autores principales: Tizhe, Daniel Thakuma, Kwaga, Jacob Kwada Paghi, Nok Kia, Grace Sabo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505854/
https://www.ncbi.nlm.nih.gov/pubmed/36146485
http://dx.doi.org/10.3390/vaccines10091407
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author Tizhe, Daniel Thakuma
Kwaga, Jacob Kwada Paghi
Nok Kia, Grace Sabo
author_facet Tizhe, Daniel Thakuma
Kwaga, Jacob Kwada Paghi
Nok Kia, Grace Sabo
author_sort Tizhe, Daniel Thakuma
collection PubMed
description Dengue is a disease caused by the dengue virus that is primarily transmitted by Aedes aegypti mosquitoes. Currently, the disease poses a threat to public health, with about 390 million people reported to be infected annually across the endemic regions of the world. In Nigeria, the disease is under-reported and often misdiagnosed as malaria. This study was designed to conduct a serological and molecular survey for dengue virus infection in febrile patients in three Local Government Areas (LGAs) in Adamawa State, Nigeria, from September through December 2020. Serum samples from 424 patients were analysed by Enzyme-Linked Immunosorbent Assay (CALBIOTECH, Dengue Virus IgM ELISA). Thick and thin smear microscopic techniques were used to determine the presence of malaria parasites. Overall, 19.4% patients were sero-positive for dengue in the three study locations. A total of 11%, 14.5% and 12.3% participants were found to be co-infected with dengue and malaria in Mubi, Jimeta and Numan, respectively. The CDC DENV1-4 RT-PCR Assay reagent was used for serotype-specific detection and identification of circulating serotypes. From the ELISA-positive samples, 11 (2.6%) cases were confirmed to be dengue serotype 1 by Real-Time PCR and sequencing and were found to be in circulation in all the three study areas. With an overall sero-prevalence of 19.4%, dengue virus infection may be one of the major causes of febrile illnesses across the study locations; hence, public healthcare professionals should not neglect other aetiologies of febrile illnesses and the need to conduct laboratory diagnoses to determine the possible causes of febrile illnesses.
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spelling pubmed-95058542022-09-24 Serological and Molecular Survey for Dengue Virus Infection in Suspected Febrile Patients in Selected Local Government Areas in Adamawa State, Nigeria Tizhe, Daniel Thakuma Kwaga, Jacob Kwada Paghi Nok Kia, Grace Sabo Vaccines (Basel) Article Dengue is a disease caused by the dengue virus that is primarily transmitted by Aedes aegypti mosquitoes. Currently, the disease poses a threat to public health, with about 390 million people reported to be infected annually across the endemic regions of the world. In Nigeria, the disease is under-reported and often misdiagnosed as malaria. This study was designed to conduct a serological and molecular survey for dengue virus infection in febrile patients in three Local Government Areas (LGAs) in Adamawa State, Nigeria, from September through December 2020. Serum samples from 424 patients were analysed by Enzyme-Linked Immunosorbent Assay (CALBIOTECH, Dengue Virus IgM ELISA). Thick and thin smear microscopic techniques were used to determine the presence of malaria parasites. Overall, 19.4% patients were sero-positive for dengue in the three study locations. A total of 11%, 14.5% and 12.3% participants were found to be co-infected with dengue and malaria in Mubi, Jimeta and Numan, respectively. The CDC DENV1-4 RT-PCR Assay reagent was used for serotype-specific detection and identification of circulating serotypes. From the ELISA-positive samples, 11 (2.6%) cases were confirmed to be dengue serotype 1 by Real-Time PCR and sequencing and were found to be in circulation in all the three study areas. With an overall sero-prevalence of 19.4%, dengue virus infection may be one of the major causes of febrile illnesses across the study locations; hence, public healthcare professionals should not neglect other aetiologies of febrile illnesses and the need to conduct laboratory diagnoses to determine the possible causes of febrile illnesses. MDPI 2022-08-28 /pmc/articles/PMC9505854/ /pubmed/36146485 http://dx.doi.org/10.3390/vaccines10091407 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tizhe, Daniel Thakuma
Kwaga, Jacob Kwada Paghi
Nok Kia, Grace Sabo
Serological and Molecular Survey for Dengue Virus Infection in Suspected Febrile Patients in Selected Local Government Areas in Adamawa State, Nigeria
title Serological and Molecular Survey for Dengue Virus Infection in Suspected Febrile Patients in Selected Local Government Areas in Adamawa State, Nigeria
title_full Serological and Molecular Survey for Dengue Virus Infection in Suspected Febrile Patients in Selected Local Government Areas in Adamawa State, Nigeria
title_fullStr Serological and Molecular Survey for Dengue Virus Infection in Suspected Febrile Patients in Selected Local Government Areas in Adamawa State, Nigeria
title_full_unstemmed Serological and Molecular Survey for Dengue Virus Infection in Suspected Febrile Patients in Selected Local Government Areas in Adamawa State, Nigeria
title_short Serological and Molecular Survey for Dengue Virus Infection in Suspected Febrile Patients in Selected Local Government Areas in Adamawa State, Nigeria
title_sort serological and molecular survey for dengue virus infection in suspected febrile patients in selected local government areas in adamawa state, nigeria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505854/
https://www.ncbi.nlm.nih.gov/pubmed/36146485
http://dx.doi.org/10.3390/vaccines10091407
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