Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome

SIMPLE SUMMARY: Raw brewers’ spent grain, a by-product of beer production, is produced at a large scale and is usually used as animal feed or is landfilled. However, its composition shows that this feedstock has the potential for other applications, such as bioplastics production (e.g., polyhydroxyalkanoates). In this way, the aim of this work was to assess the use of raw brewers’ spent grain for polyhydroxyalkanoates production, adding new value to this feedstock. The results confirm the potential of raw brewers’ spent grain to produce polyhydroxyalkanoates, as the population was enriched in the microorganisms able to accumulate these biopolymers. These results will contribute to society’s knowledge and competence via the development of a treatment process for brewery waste of both environmental (productive waste treatment) and economic interest (production of biopolymers), which will certainly attract its application to the brewery industry worldwide. ABSTRACT: Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 Cmmol(PHA)/Cmmol(VFA) in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.