Cargando…

Discovering a Multi-Component Combination against Vascular Dementia from Danshen-Honghua Herbal Pair by Spectrum-Effect Relationship Analysis

The Danshen-Honghua (DH) herbal pair exhibits a synergistic effect in protecting the cerebrovascular system from ischemia/reperfusion injury, but the therapeutic effect on vascular dementia (VaD) has not been clarified, and the main active ingredient group has not been clarified. In this work, the c...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Peilin, He, Shiru, Wu, Siqi, Li, Yi, Wang, Huiying, Yan, Changyang, Yang, Hua, Li, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505896/
https://www.ncbi.nlm.nih.gov/pubmed/36145294
http://dx.doi.org/10.3390/ph15091073
_version_ 1784796587709431808
author Zhang, Peilin
He, Shiru
Wu, Siqi
Li, Yi
Wang, Huiying
Yan, Changyang
Yang, Hua
Li, Ping
author_facet Zhang, Peilin
He, Shiru
Wu, Siqi
Li, Yi
Wang, Huiying
Yan, Changyang
Yang, Hua
Li, Ping
author_sort Zhang, Peilin
collection PubMed
description The Danshen-Honghua (DH) herbal pair exhibits a synergistic effect in protecting the cerebrovascular system from ischemia/reperfusion injury, but the therapeutic effect on vascular dementia (VaD) has not been clarified, and the main active ingredient group has not been clarified. In this work, the chemical constituents in DH herbal pair extract were characterized by UHPLC-QTOF MS, and a total of 72 compounds were identified. Moreover, the DH herbal pair alleviated phenylhydrazine (PHZ)-induced thrombosis and improved bisphenol F (BPF)- and ponatinib-induced brain injury in zebrafish. Furthermore, the spectrum-effect relationship between the fingerprint of the DH herbal pair and the antithrombotic and neuroprotective efficacy was analyzed, and 11 chemical components were screened out as the multi-component combination (MCC) against VaD. Among them, the two compounds with the highest content were salvianolic acid B (17.31 ± 0.20 mg/g) and hydroxysafflor yellow A (15.85 ± 0.19 mg/g). Finally, we combined these 11 candidate compounds as the MCC and found that it could improve thrombosis and neuronal injury in three zebrafish models and rat bilateral common carotid artery occlusion (BCCAO) model, which had similar efficacy compared to the DH herbal pair. This study provides research ideas for the treatment of VaD and the clinical application of the DH herbal pair.
format Online
Article
Text
id pubmed-9505896
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95058962022-09-24 Discovering a Multi-Component Combination against Vascular Dementia from Danshen-Honghua Herbal Pair by Spectrum-Effect Relationship Analysis Zhang, Peilin He, Shiru Wu, Siqi Li, Yi Wang, Huiying Yan, Changyang Yang, Hua Li, Ping Pharmaceuticals (Basel) Article The Danshen-Honghua (DH) herbal pair exhibits a synergistic effect in protecting the cerebrovascular system from ischemia/reperfusion injury, but the therapeutic effect on vascular dementia (VaD) has not been clarified, and the main active ingredient group has not been clarified. In this work, the chemical constituents in DH herbal pair extract were characterized by UHPLC-QTOF MS, and a total of 72 compounds were identified. Moreover, the DH herbal pair alleviated phenylhydrazine (PHZ)-induced thrombosis and improved bisphenol F (BPF)- and ponatinib-induced brain injury in zebrafish. Furthermore, the spectrum-effect relationship between the fingerprint of the DH herbal pair and the antithrombotic and neuroprotective efficacy was analyzed, and 11 chemical components were screened out as the multi-component combination (MCC) against VaD. Among them, the two compounds with the highest content were salvianolic acid B (17.31 ± 0.20 mg/g) and hydroxysafflor yellow A (15.85 ± 0.19 mg/g). Finally, we combined these 11 candidate compounds as the MCC and found that it could improve thrombosis and neuronal injury in three zebrafish models and rat bilateral common carotid artery occlusion (BCCAO) model, which had similar efficacy compared to the DH herbal pair. This study provides research ideas for the treatment of VaD and the clinical application of the DH herbal pair. MDPI 2022-08-29 /pmc/articles/PMC9505896/ /pubmed/36145294 http://dx.doi.org/10.3390/ph15091073 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Peilin
He, Shiru
Wu, Siqi
Li, Yi
Wang, Huiying
Yan, Changyang
Yang, Hua
Li, Ping
Discovering a Multi-Component Combination against Vascular Dementia from Danshen-Honghua Herbal Pair by Spectrum-Effect Relationship Analysis
title Discovering a Multi-Component Combination against Vascular Dementia from Danshen-Honghua Herbal Pair by Spectrum-Effect Relationship Analysis
title_full Discovering a Multi-Component Combination against Vascular Dementia from Danshen-Honghua Herbal Pair by Spectrum-Effect Relationship Analysis
title_fullStr Discovering a Multi-Component Combination against Vascular Dementia from Danshen-Honghua Herbal Pair by Spectrum-Effect Relationship Analysis
title_full_unstemmed Discovering a Multi-Component Combination against Vascular Dementia from Danshen-Honghua Herbal Pair by Spectrum-Effect Relationship Analysis
title_short Discovering a Multi-Component Combination against Vascular Dementia from Danshen-Honghua Herbal Pair by Spectrum-Effect Relationship Analysis
title_sort discovering a multi-component combination against vascular dementia from danshen-honghua herbal pair by spectrum-effect relationship analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505896/
https://www.ncbi.nlm.nih.gov/pubmed/36145294
http://dx.doi.org/10.3390/ph15091073
work_keys_str_mv AT zhangpeilin discoveringamulticomponentcombinationagainstvasculardementiafromdanshenhonghuaherbalpairbyspectrumeffectrelationshipanalysis
AT heshiru discoveringamulticomponentcombinationagainstvasculardementiafromdanshenhonghuaherbalpairbyspectrumeffectrelationshipanalysis
AT wusiqi discoveringamulticomponentcombinationagainstvasculardementiafromdanshenhonghuaherbalpairbyspectrumeffectrelationshipanalysis
AT liyi discoveringamulticomponentcombinationagainstvasculardementiafromdanshenhonghuaherbalpairbyspectrumeffectrelationshipanalysis
AT wanghuiying discoveringamulticomponentcombinationagainstvasculardementiafromdanshenhonghuaherbalpairbyspectrumeffectrelationshipanalysis
AT yanchangyang discoveringamulticomponentcombinationagainstvasculardementiafromdanshenhonghuaherbalpairbyspectrumeffectrelationshipanalysis
AT yanghua discoveringamulticomponentcombinationagainstvasculardementiafromdanshenhonghuaherbalpairbyspectrumeffectrelationshipanalysis
AT liping discoveringamulticomponentcombinationagainstvasculardementiafromdanshenhonghuaherbalpairbyspectrumeffectrelationshipanalysis