Cargando…

Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common genetic disorder among those responsible for hereditary strokes, and it is caused by a mutation in the NOTCH3 gene on chromosome 19. Blood biomarkers related to the Notch signaling...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Hyesung, Jang, Bogun, Kim, Yang-Ji, Choi, Jay Chol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505916/
https://www.ncbi.nlm.nih.gov/pubmed/36142458
http://dx.doi.org/10.3390/ijms231810547
_version_ 1784796592710090752
author Kim, Hyesung
Jang, Bogun
Kim, Yang-Ji
Choi, Jay Chol
author_facet Kim, Hyesung
Jang, Bogun
Kim, Yang-Ji
Choi, Jay Chol
author_sort Kim, Hyesung
collection PubMed
description Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common genetic disorder among those responsible for hereditary strokes, and it is caused by a mutation in the NOTCH3 gene on chromosome 19. Blood biomarkers related to the Notch signaling pathway have not been investigated extensively in CADASIL. In this study, we measured the serum and plasma levels of NOTCH3 extracellular domain (N3ECD) and its ligand, Jagged-1, in 279 healthy subjects. The levels of N3ECD and Jagged-1 showed significant correlations in both serum (p < 0.0001, r = 0.2681) and plasma (p < 0.0001, r = 0.4065). The N3ECD levels were significantly higher in the serum than in plasma and tend to increase with age. In contrast, there was no significant difference between the serum and plasma levels of Jagged-1 levels. To summarize, we were able to measure N3ECD and Jagged-1 protein levels in healthy human serum and plasma. Taken together, our findings provide the basis for further studies investigating the clinical use of blood N3ECD and Jagged-1 levels for CADASIL and other Notch signaling-related diseases.
format Online
Article
Text
id pubmed-9505916
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95059162022-09-24 Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects Kim, Hyesung Jang, Bogun Kim, Yang-Ji Choi, Jay Chol Int J Mol Sci Article Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common genetic disorder among those responsible for hereditary strokes, and it is caused by a mutation in the NOTCH3 gene on chromosome 19. Blood biomarkers related to the Notch signaling pathway have not been investigated extensively in CADASIL. In this study, we measured the serum and plasma levels of NOTCH3 extracellular domain (N3ECD) and its ligand, Jagged-1, in 279 healthy subjects. The levels of N3ECD and Jagged-1 showed significant correlations in both serum (p < 0.0001, r = 0.2681) and plasma (p < 0.0001, r = 0.4065). The N3ECD levels were significantly higher in the serum than in plasma and tend to increase with age. In contrast, there was no significant difference between the serum and plasma levels of Jagged-1 levels. To summarize, we were able to measure N3ECD and Jagged-1 protein levels in healthy human serum and plasma. Taken together, our findings provide the basis for further studies investigating the clinical use of blood N3ECD and Jagged-1 levels for CADASIL and other Notch signaling-related diseases. MDPI 2022-09-11 /pmc/articles/PMC9505916/ /pubmed/36142458 http://dx.doi.org/10.3390/ijms231810547 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Hyesung
Jang, Bogun
Kim, Yang-Ji
Choi, Jay Chol
Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
title Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
title_full Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
title_fullStr Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
title_full_unstemmed Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
title_short Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
title_sort determination of blood notch3 extracellular domain and jagged-1 levels in healthy subjects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505916/
https://www.ncbi.nlm.nih.gov/pubmed/36142458
http://dx.doi.org/10.3390/ijms231810547
work_keys_str_mv AT kimhyesung determinationofbloodnotch3extracellulardomainandjagged1levelsinhealthysubjects
AT jangbogun determinationofbloodnotch3extracellulardomainandjagged1levelsinhealthysubjects
AT kimyangji determinationofbloodnotch3extracellulardomainandjagged1levelsinhealthysubjects
AT choijaychol determinationofbloodnotch3extracellulardomainandjagged1levelsinhealthysubjects