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The Effect of the BCO2 Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract

This study investigated the effect of the BCO2 genotype and the addition of Aztec marigold flower extract to rabbit diets on the expression of BCO1, BCO2, LRAT, and TTPA genes in the liver. The levels of lutein, zeaxanthin, β-carotene, retinol, and α-tocopherol in the liver and blood serum of rabbit...

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Autores principales: Strychalski, Janusz, Gugołek, Andrzej, Antoszkiewicz, Zofia, Fopp-Bayat, Dorota, Kaczorek-Łukowska, Edyta, Snarska, Anna, Zwierzchowski, Grzegorz, Król-Grzymała, Angelika, Matusevičius, Paulius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506012/
https://www.ncbi.nlm.nih.gov/pubmed/36142463
http://dx.doi.org/10.3390/ijms231810552
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author Strychalski, Janusz
Gugołek, Andrzej
Antoszkiewicz, Zofia
Fopp-Bayat, Dorota
Kaczorek-Łukowska, Edyta
Snarska, Anna
Zwierzchowski, Grzegorz
Król-Grzymała, Angelika
Matusevičius, Paulius
author_facet Strychalski, Janusz
Gugołek, Andrzej
Antoszkiewicz, Zofia
Fopp-Bayat, Dorota
Kaczorek-Łukowska, Edyta
Snarska, Anna
Zwierzchowski, Grzegorz
Król-Grzymała, Angelika
Matusevičius, Paulius
author_sort Strychalski, Janusz
collection PubMed
description This study investigated the effect of the BCO2 genotype and the addition of Aztec marigold flower extract to rabbit diets on the expression of BCO1, BCO2, LRAT, and TTPA genes in the liver. The levels of lutein, zeaxanthin, β-carotene, retinol, and α-tocopherol in the liver and blood serum of rabbits, as well as plasma biochemical parameters and serum antioxidant enzyme activities were also determined. Sixty male Termond White growing rabbits were divided into three groups based on their genotype at codon 248 of the BCO2 gene (ins/ins, ins/del and del/del); each group was divided into two subgroups: one subgroup received a standard diet, and the other subgroup was fed a diet supplemented with 6 g/kg of marigold flower extract. The obtained results indicate that the BCO2 genotype may affect the expression levels of BCO1 and BCO2 genes in rabbits. Moreover, the addition of marigold extract to the diet of BCO2 del/del rabbits may increase the expression level of the BCO2 gene. Finally, an increase in the amount of lutein in the diet of rabbits with the BCO2 del/del genotype contributes to its increased accumulation in the liver and blood of animals without compromising their health status or liver function.
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spelling pubmed-95060122022-09-24 The Effect of the BCO2 Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract Strychalski, Janusz Gugołek, Andrzej Antoszkiewicz, Zofia Fopp-Bayat, Dorota Kaczorek-Łukowska, Edyta Snarska, Anna Zwierzchowski, Grzegorz Król-Grzymała, Angelika Matusevičius, Paulius Int J Mol Sci Article This study investigated the effect of the BCO2 genotype and the addition of Aztec marigold flower extract to rabbit diets on the expression of BCO1, BCO2, LRAT, and TTPA genes in the liver. The levels of lutein, zeaxanthin, β-carotene, retinol, and α-tocopherol in the liver and blood serum of rabbits, as well as plasma biochemical parameters and serum antioxidant enzyme activities were also determined. Sixty male Termond White growing rabbits were divided into three groups based on their genotype at codon 248 of the BCO2 gene (ins/ins, ins/del and del/del); each group was divided into two subgroups: one subgroup received a standard diet, and the other subgroup was fed a diet supplemented with 6 g/kg of marigold flower extract. The obtained results indicate that the BCO2 genotype may affect the expression levels of BCO1 and BCO2 genes in rabbits. Moreover, the addition of marigold extract to the diet of BCO2 del/del rabbits may increase the expression level of the BCO2 gene. Finally, an increase in the amount of lutein in the diet of rabbits with the BCO2 del/del genotype contributes to its increased accumulation in the liver and blood of animals without compromising their health status or liver function. MDPI 2022-09-11 /pmc/articles/PMC9506012/ /pubmed/36142463 http://dx.doi.org/10.3390/ijms231810552 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Strychalski, Janusz
Gugołek, Andrzej
Antoszkiewicz, Zofia
Fopp-Bayat, Dorota
Kaczorek-Łukowska, Edyta
Snarska, Anna
Zwierzchowski, Grzegorz
Król-Grzymała, Angelika
Matusevičius, Paulius
The Effect of the BCO2 Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract
title The Effect of the BCO2 Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract
title_full The Effect of the BCO2 Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract
title_fullStr The Effect of the BCO2 Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract
title_full_unstemmed The Effect of the BCO2 Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract
title_short The Effect of the BCO2 Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract
title_sort effect of the bco2 genotype on the expression of genes related to carotenoid, retinol, and α-tocopherol metabolism in rabbits fed a diet with aztec marigold flower extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506012/
https://www.ncbi.nlm.nih.gov/pubmed/36142463
http://dx.doi.org/10.3390/ijms231810552
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