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Pattern of Mitochondrial Respiration in Peripheral Blood Cells of Patients with Parkinson’s Disease

Mitochondria are central in the pathogenesis of Parkinson’s disease (PD), as they are involved in oxidative stress, synaptopathy, and other immunometabolic pathways. Accordingly, they are emerging as a potential neuroprotection target, although further human-based evidence is needed for therapeutic...

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Autores principales: Schirinzi, Tommaso, Salvatori, Illari, Zenuni, Henri, Grillo, Piergiorgio, Valle, Cristiana, Martella, Giuseppina, Mercuri, Nicola Biagio, Ferri, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506016/
https://www.ncbi.nlm.nih.gov/pubmed/36142777
http://dx.doi.org/10.3390/ijms231810863
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author Schirinzi, Tommaso
Salvatori, Illari
Zenuni, Henri
Grillo, Piergiorgio
Valle, Cristiana
Martella, Giuseppina
Mercuri, Nicola Biagio
Ferri, Alberto
author_facet Schirinzi, Tommaso
Salvatori, Illari
Zenuni, Henri
Grillo, Piergiorgio
Valle, Cristiana
Martella, Giuseppina
Mercuri, Nicola Biagio
Ferri, Alberto
author_sort Schirinzi, Tommaso
collection PubMed
description Mitochondria are central in the pathogenesis of Parkinson’s disease (PD), as they are involved in oxidative stress, synaptopathy, and other immunometabolic pathways. Accordingly, they are emerging as a potential neuroprotection target, although further human-based evidence is needed for therapeutic advancements. This study aims to shape the pattern of mitochondrial respiration in the blood leukocytes of PD patients in relation to both clinical features and the profile of cerebrospinal fluid (CSF) biomarkers of neurodegeneration. Mitochondrial respirometry on the peripheral blood mononucleate cells (PBMCs) of 16 PD patients and 14 controls was conducted using Seahorse Bioscience technology. Bioenergetic parameters were correlated either with standard clinical scores for motor and non-motor disturbances or with CSF levels of α-synuclein, amyloid-β peptides, and tau proteins. In PD, PBMC mitochondrial basal respiration was normal; maximal and spare respiratory capacities were both increased; and ATP production was higher, although not significantly. Maximal and spare respiratory capacity was directly correlated with disease duration, MDS-UPDRS part III and Hoehn and Yahr motor scores; spare respiratory capacity was correlated with the CSF amyloid-β-42 to amyloid-β-42/40 ratio. We provided preliminary evidence showing that mitochondrial respiratory activity increases in the PBMCs of PD patients, probably following the compensatory adaptations to disease progression, in contrast to the bases of the neuropathological substrate.
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spelling pubmed-95060162022-09-24 Pattern of Mitochondrial Respiration in Peripheral Blood Cells of Patients with Parkinson’s Disease Schirinzi, Tommaso Salvatori, Illari Zenuni, Henri Grillo, Piergiorgio Valle, Cristiana Martella, Giuseppina Mercuri, Nicola Biagio Ferri, Alberto Int J Mol Sci Communication Mitochondria are central in the pathogenesis of Parkinson’s disease (PD), as they are involved in oxidative stress, synaptopathy, and other immunometabolic pathways. Accordingly, they are emerging as a potential neuroprotection target, although further human-based evidence is needed for therapeutic advancements. This study aims to shape the pattern of mitochondrial respiration in the blood leukocytes of PD patients in relation to both clinical features and the profile of cerebrospinal fluid (CSF) biomarkers of neurodegeneration. Mitochondrial respirometry on the peripheral blood mononucleate cells (PBMCs) of 16 PD patients and 14 controls was conducted using Seahorse Bioscience technology. Bioenergetic parameters were correlated either with standard clinical scores for motor and non-motor disturbances or with CSF levels of α-synuclein, amyloid-β peptides, and tau proteins. In PD, PBMC mitochondrial basal respiration was normal; maximal and spare respiratory capacities were both increased; and ATP production was higher, although not significantly. Maximal and spare respiratory capacity was directly correlated with disease duration, MDS-UPDRS part III and Hoehn and Yahr motor scores; spare respiratory capacity was correlated with the CSF amyloid-β-42 to amyloid-β-42/40 ratio. We provided preliminary evidence showing that mitochondrial respiratory activity increases in the PBMCs of PD patients, probably following the compensatory adaptations to disease progression, in contrast to the bases of the neuropathological substrate. MDPI 2022-09-17 /pmc/articles/PMC9506016/ /pubmed/36142777 http://dx.doi.org/10.3390/ijms231810863 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Schirinzi, Tommaso
Salvatori, Illari
Zenuni, Henri
Grillo, Piergiorgio
Valle, Cristiana
Martella, Giuseppina
Mercuri, Nicola Biagio
Ferri, Alberto
Pattern of Mitochondrial Respiration in Peripheral Blood Cells of Patients with Parkinson’s Disease
title Pattern of Mitochondrial Respiration in Peripheral Blood Cells of Patients with Parkinson’s Disease
title_full Pattern of Mitochondrial Respiration in Peripheral Blood Cells of Patients with Parkinson’s Disease
title_fullStr Pattern of Mitochondrial Respiration in Peripheral Blood Cells of Patients with Parkinson’s Disease
title_full_unstemmed Pattern of Mitochondrial Respiration in Peripheral Blood Cells of Patients with Parkinson’s Disease
title_short Pattern of Mitochondrial Respiration in Peripheral Blood Cells of Patients with Parkinson’s Disease
title_sort pattern of mitochondrial respiration in peripheral blood cells of patients with parkinson’s disease
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506016/
https://www.ncbi.nlm.nih.gov/pubmed/36142777
http://dx.doi.org/10.3390/ijms231810863
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