Cargando…

Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate

Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport. By localizing the electric field over small portions of the cell memb...

Descripción completa

Detalles Bibliográficos
Autores principales:	Vindiš, Tina, Blažič, Anja, Khayyat, Diaa, Potočnik, Tjaša, Sachdev, Shaurya, Rems, Lea
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506064/ https://www.ncbi.nlm.nih.gov/pubmed/36145709 http://dx.doi.org/10.3390/pharmaceutics14091959

Ejemplares similares

Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro
por: Lesueur, Léa L, et al.
Publicado: (2016)

Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells
por: Pasquet, L., et al.
Publicado: (2016)

Tracking in vitro and in vivo siRNA electrotransfer in tumor cells
por: Paganin-Gioanni, Aurelie, et al.
Publicado: (2008)

Gene Electrotransfer: A Mechanistic Perspective
por: Rosazza, Christelle, et al.
Publicado: (2016)

Acute Effects of Intratumor DNA Electrotransfer
por: Bhandary, Manya, et al.
Publicado: (2022)

Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells
por: Freund, Guillaume, et al.
Publicado: (2013)

Surface Optimization of Commercial Porous Ti Substrates by EPD of Titanium Nitride
por: Mortalò, Cecilia, et al.
Publicado: (2022)

Numerical optimization of gene electrotransfer into muscle tissue
por: Zupanic, Anze, et al.
Publicado: (2010)

Development of Tumor Cell-Based Vaccine with IL-12 Gene Electrotransfer as Adjuvant
por: Remic, Tinkara, et al.
Publicado: (2020)

Design, Development, and Testing of a Device for Gene Electrotransfer to Skin Cells In Vivo
por: Cvetkoska, Aleksandra, et al.
Publicado: (2022)

Muscle gene electrotransfer is increased by the antioxidant tempol in mice
por: Markelc, B, et al.
Publicado: (2012)

Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
por: Sersa, Gregor, et al.
Publicado: (2015)

Gene electrotransfer enhanced by nanosecond pulsed electric fields
por: Guo, Siqi, et al.
Publicado: (2014)

In Vivo and Ex Vivo Gene Electrotransfer in Ophthalmological Disorders
por: Fusco, Roberta, et al.
Publicado: (2022)

Pipette tip with integrated electrodes for gene electrotransfer of cells in suspension: a feasibility study in CHO cells
por: Rebersek, Matej, et al.
Publicado: (2011)

Gene expression profiles in skeletal muscle after gene electrotransfer
por: Hojman, Pernille, et al.
Publicado: (2007)

Endocytosis and Endosomal Trafficking of DNA After Gene Electrotransfer In Vitro
por: Rosazza, Christelle, et al.
Publicado: (2016)

New Insights into the Mechanisms of Gene Electrotransfer – Experimental and Theoretical Analysis
por: Pavlin, Mojca, et al.
Publicado: (2015)

The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation
por: Ruzgys, Paulius, et al.
Publicado: (2021)

Tumor cell death after electrotransfer of plasmid DNA is associated with cytosolic DNA sensor upregulation
por: Znidar, Katarina, et al.
Publicado: (2018)

Radiosensitizing effect of intratumoral interleukin-12 gene electrotransfer in murine sarcoma
por: Sedlar, Ales, et al.
Publicado: (2013)

Sub-cellular temporal and spatial distribution of electrotransferred LNA/DNA oligomer
por: Orio, Julie, et al.
Publicado: (2013)

Electrotransfer parameters as a tool for controlled and targeted gene expression in skin
por: Kos, Spela, et al.
Publicado: (2016)

Safe and efficient novel approach for non-invasive gene electrotransfer to skin
por: Pasquet, Lise, et al.
Publicado: (2018)

Tumor Radiosensitization by Gene Electrotransfer-Mediated Double Targeting of Tumor Vasculature
por: Savarin, Monika, et al.
Publicado: (2023)

Bridging the scales in high-throughput dielectrophoretic (bio-)particle separation in porous media
por: Pesch, Georg R., et al.
Publicado: (2018)

Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells
por: Znidar, Katarina, et al.
Publicado: (2016)

Electrochemotherapy with bleomycin is effective in BRAF mutated melanoma cells and interacts with BRAF inhibitors
por: Dolinsek, Tanja, et al.
Publicado: (2016)

Optical imaging of luminescence for in vivo quantification of gene electrotransfer in mouse muscle and knee
por: Bloquel, C, et al.
Publicado: (2006)

Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro
por: Reberšek, Matej, et al.
Publicado: (2007)

High efficiency intramuscular plasmid electrotransfer: application for sustained plasmatic secretion of cytokines
por: Scherman, D, et al.
Publicado: (2001)

Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array
por: Ferraro, Bernadette, et al.
Publicado: (2010)

OA05-03. Efficacy study of a T-cell-based DNA vaccine delivered by intradermal electrotransfer in macaques
por: Dereuddre-Bosquet, N, et al.
Publicado: (2009)

Upregulation of DNA Sensors in B16.F10 Melanoma Spheroid Cells After Electrotransfer of pDNA
por: Znidar, Katarina, et al.
Publicado: (2018)

Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model
por: Vadeikienė, Roberta, et al.
Publicado: (2022)

Vero Cells as a Mammalian Cell Substrate for Human Norovirus
por: Todd, Kyle V., et al.
Publicado: (2020)

A universal mammalian vaccine cell line substrate
por: Murray, Jackelyn, et al.
Publicado: (2017)

Cell Colonization Ability of a Commercialized Large Porous Alveolar Scaffold
por: Lemonnier, S., et al.
Publicado: (2017)

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold
por: Sieni, Elisabetta, et al.
Publicado: (2020)

Non viral gene therapy in arthritis by in vivo intramuscular IL-10 DNA electrotransfer
por: Jorgensen, CJ, et al.
Publicado: (2002)

Cannot write session to /tmp/vufind_sessions/sess_ujj14e277dh9ge3h8q44idb6eu