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Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms
The mechanism by which folic acid (FA) or its derivatives (folates) mediates plant tolerance to sodic-alkaline stress has not been clarified in previous literature. To apply sodic-alkaline stress, maize seedlings were irrigated with 50 mM of a combined solution (1:1) of sodic-alkaline salts (NaHCO(3...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506096/ https://www.ncbi.nlm.nih.gov/pubmed/36143364 http://dx.doi.org/10.3390/life12091327 |
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author | Aljuaid, Bandar S. Mukherjee, Soumya Sayed, Amany N. El-Gabry, Yasser Abd El-Gawad Omar, Mohamed M. A. Mahmoud, Samy F. Alsubeie, Moodi Saham Darwish, Doaa Bahaa Eldin Al-Qahtani, Salem Mesfir Al-Harbi, Nadi Awad Alzuaibr, Fahad Mohammed Basahi, Mohammed A. Hamada, Maha M. A. |
author_facet | Aljuaid, Bandar S. Mukherjee, Soumya Sayed, Amany N. El-Gabry, Yasser Abd El-Gawad Omar, Mohamed M. A. Mahmoud, Samy F. Alsubeie, Moodi Saham Darwish, Doaa Bahaa Eldin Al-Qahtani, Salem Mesfir Al-Harbi, Nadi Awad Alzuaibr, Fahad Mohammed Basahi, Mohammed A. Hamada, Maha M. A. |
author_sort | Aljuaid, Bandar S. |
collection | PubMed |
description | The mechanism by which folic acid (FA) or its derivatives (folates) mediates plant tolerance to sodic-alkaline stress has not been clarified in previous literature. To apply sodic-alkaline stress, maize seedlings were irrigated with 50 mM of a combined solution (1:1) of sodic-alkaline salts (NaHCO(3) and Na(2)CO(3); pH 9.7). Maize seedlings under stressed and non-stressed conditions were sprayed with folic acid (FA) at 0 (distilled water as control), 0.05, 0.1, and 0.2 mM. Under sodic-alkaline stress, FA applied at 0.2 mM significantly improved shoot fresh weight (95%), chlorophyll (Chl a (41%), Chl b (57%), and total Chl (42%)), and carotenoids (27%) compared to the untreated plants, while root fresh weight was not affected compared to the untreated plants. This improvement was associated with a significant enhancement in the cell-membrane stability index (CMSI), relative water content (RWC), free amino acids (FAA), proline, soluble sugars, K, and Ca. In contrast, Na, Na/K ratio, H(2)O(2), malondialdehyde (MDA), and methylglycoxal (MG) were significantly decreased. Moreover, seedlings treated with FA demonstrated significantly higher activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and ascorbate peroxidase (APX) compared to the untreated plants. The molecular studies using RT-qPCR demonstrated that FA treatments, specifically at 0.2 mM, enhanced the K(+)/Na(+) selectivity and the performance of photosynthesis under alkaline-stress conditions. These responses were observed through up-regulation of the expression of the high-affinity potassium-transporter protein (ZmHKT1), the major core protein of photosystem II (D2-Protein), and the activity of the first enzyme of carbon fixation cycle in C4 plants (PEP-case) by 74, 248, and 225% over the untreated plants, respectively. Conversely, there was a significant down-regulation in the expression ZmSOS1 and ZmNHX1 by 48.2 and 27.8%, respectively, compared to the untreated plants. |
format | Online Article Text |
id | pubmed-9506096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95060962022-09-24 Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms Aljuaid, Bandar S. Mukherjee, Soumya Sayed, Amany N. El-Gabry, Yasser Abd El-Gawad Omar, Mohamed M. A. Mahmoud, Samy F. Alsubeie, Moodi Saham Darwish, Doaa Bahaa Eldin Al-Qahtani, Salem Mesfir Al-Harbi, Nadi Awad Alzuaibr, Fahad Mohammed Basahi, Mohammed A. Hamada, Maha M. A. Life (Basel) Article The mechanism by which folic acid (FA) or its derivatives (folates) mediates plant tolerance to sodic-alkaline stress has not been clarified in previous literature. To apply sodic-alkaline stress, maize seedlings were irrigated with 50 mM of a combined solution (1:1) of sodic-alkaline salts (NaHCO(3) and Na(2)CO(3); pH 9.7). Maize seedlings under stressed and non-stressed conditions were sprayed with folic acid (FA) at 0 (distilled water as control), 0.05, 0.1, and 0.2 mM. Under sodic-alkaline stress, FA applied at 0.2 mM significantly improved shoot fresh weight (95%), chlorophyll (Chl a (41%), Chl b (57%), and total Chl (42%)), and carotenoids (27%) compared to the untreated plants, while root fresh weight was not affected compared to the untreated plants. This improvement was associated with a significant enhancement in the cell-membrane stability index (CMSI), relative water content (RWC), free amino acids (FAA), proline, soluble sugars, K, and Ca. In contrast, Na, Na/K ratio, H(2)O(2), malondialdehyde (MDA), and methylglycoxal (MG) were significantly decreased. Moreover, seedlings treated with FA demonstrated significantly higher activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and ascorbate peroxidase (APX) compared to the untreated plants. The molecular studies using RT-qPCR demonstrated that FA treatments, specifically at 0.2 mM, enhanced the K(+)/Na(+) selectivity and the performance of photosynthesis under alkaline-stress conditions. These responses were observed through up-regulation of the expression of the high-affinity potassium-transporter protein (ZmHKT1), the major core protein of photosystem II (D2-Protein), and the activity of the first enzyme of carbon fixation cycle in C4 plants (PEP-case) by 74, 248, and 225% over the untreated plants, respectively. Conversely, there was a significant down-regulation in the expression ZmSOS1 and ZmNHX1 by 48.2 and 27.8%, respectively, compared to the untreated plants. MDPI 2022-08-27 /pmc/articles/PMC9506096/ /pubmed/36143364 http://dx.doi.org/10.3390/life12091327 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aljuaid, Bandar S. Mukherjee, Soumya Sayed, Amany N. El-Gabry, Yasser Abd El-Gawad Omar, Mohamed M. A. Mahmoud, Samy F. Alsubeie, Moodi Saham Darwish, Doaa Bahaa Eldin Al-Qahtani, Salem Mesfir Al-Harbi, Nadi Awad Alzuaibr, Fahad Mohammed Basahi, Mohammed A. Hamada, Maha M. A. Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms |
title | Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms |
title_full | Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms |
title_fullStr | Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms |
title_full_unstemmed | Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms |
title_short | Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms |
title_sort | folic acid reinforces maize tolerance to sodic-alkaline stress through modulation of growth, biochemical and molecular mechanisms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506096/ https://www.ncbi.nlm.nih.gov/pubmed/36143364 http://dx.doi.org/10.3390/life12091327 |
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