Biochar and Cropping Systems Changed Soil Copper Speciation and Accumulation in Sweet Corn and Soybean

In order to explore the effects of biochar and cropping systems on soil copper (Cu) speciation and copper accumulation in sweet corn (Zea mays L. var. Rugosa Bonaf.) and soybean (Glycine max (L.) Merr.), three ratios of biochar (C0, 0%, C1, 2%, C2, 5% by mass ratio, (w/w)) and three cropping systems (monocropped sweet corn, MC; monocropped soybean, MS; sweet corn–soybean intercropping, CS) were studied under three Cu levels (Cu0, 0 mg·kg(−1), Cu1, 200 mg·kg(−1), and Cu2, 400 mg·kg(−1)) in a pot experiment. The following results were obtained: (1) Compared with C0, adding biochar (C1, C2) could significantly reduce the Cu concentration in sweet corn, and C2 significantly reduced the Cu concentration in soybean under Cu1 and Cu2; the Cu concentrations in sweet corn and soybeans under Cu1 were lower than 10 mg·kg(−1). (2) Compared with MC or MS, C2 significantly reduced the Cu concentration (below the detection limit) in sweet corn and the Cu concentration (1.65 mg·kg(−1)) in soybean straw in CS under Cu1. The Cu concentration in sweet corn ears and soybean straw in CS under Cu2 also decreased significantly, reaching 1.84 and 10.36 mg·kg(−1), respectively. (3) Compared with C0, C2 significantly reduced the soil acid-soluble Cu concentration under Cu1 and Cu2, but significantly increased soil oxidated Cu concentration. (4) Compared with MC, the concentration of soil acid-soluble Cu was significantly decreased in CSC1 under Cu2. Under Cu1, the concentrations of reducible Cu were significantly increased in CSC1 and CSC2, and the oxidizable Cu concentration was increased in CSC2. In conclusion, sweet corn–soybean intercropping combined with biochar 5% (w/w) is beneficial to reducing the concentration of acid-soluble Cu, and increases the concentration of oxidizable Cu in copper-contaminated soil. Under Cu1 (200 mg·kg(−1)), the Cu concentrations in sweet corn and soybean were lower than 10 mg·kg(−1), which meets the national food safety standard of China. Under Cu2 (400 mg·kg(−1)), the Cu concentration in sweet corn was lower than 10 mg·kg(−1), but it was higher than 10 mg·kg(−1) in soybean.