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Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass

The frequent occurrence of heat and drought stress can severely reduce agricultural production of field crops. In comparison to a single stress, the combination of both heat (H) and drought (D) further reduce plant growth, survival and yield. This study aimed to explore the transcriptional responses...

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Autores principales: Rahman, Md Atikur, Woo, Jae Hoon, Song, Yowook, Lee, Sang-Hoon, Hasan, Md Mahadi, Azad, Md Abul Kalam, Lee, Ki-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506360/
https://www.ncbi.nlm.nih.gov/pubmed/36143461
http://dx.doi.org/10.3390/life12091426
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author Rahman, Md Atikur
Woo, Jae Hoon
Song, Yowook
Lee, Sang-Hoon
Hasan, Md Mahadi
Azad, Md Abul Kalam
Lee, Ki-Won
author_facet Rahman, Md Atikur
Woo, Jae Hoon
Song, Yowook
Lee, Sang-Hoon
Hasan, Md Mahadi
Azad, Md Abul Kalam
Lee, Ki-Won
author_sort Rahman, Md Atikur
collection PubMed
description The frequent occurrence of heat and drought stress can severely reduce agricultural production of field crops. In comparison to a single stress, the combination of both heat (H) and drought (D) further reduce plant growth, survival and yield. This study aimed to explore the transcriptional responses of heat shock protein (HSP) and antioxidant genes under H combined D stress in perennial rye grass (PRG). The results demonstrated that oxidative stress indicators (hydrogen peroxide, lipid peroxidation) significantly increased, particularly in the case of combined H and D treatment, suggesting that oxidative stress-induced damage occurred in plants under the combined stresses. Transcriptional responses of heat shock protein 70 (HSP70), heat shock protein 90-6 (HSP90-6), and the mitochondrial small heat shock protein HSP26.2 (HSP26.2) occurred rapidly, and showed high level of expression particularly under H and D stress. Antioxidant genes including ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), catalase (CAT), copper–zinc superoxide dismutase (Cu/ZnSOD), peroxidase (POD), ferredoxin–thioredoxin (FTR), thioredoxin (Trx), 2-cysteine peroxiredoxin (2-Cys Prx) showed response to combined H and D, followed by either D or H stress alone in rye grass. An interactome map revealed the close partnership of these heat shock protein genes and antioxidant genes, respectively. These candidate genes were predominantly linked to stress responses and antioxidant defense in plants. These findings may advance our understanding about the HSP and the antioxidant genes underlying combined abiotic stress response and tolerance in perennial rye grass.
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spelling pubmed-95063602022-09-24 Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass Rahman, Md Atikur Woo, Jae Hoon Song, Yowook Lee, Sang-Hoon Hasan, Md Mahadi Azad, Md Abul Kalam Lee, Ki-Won Life (Basel) Article The frequent occurrence of heat and drought stress can severely reduce agricultural production of field crops. In comparison to a single stress, the combination of both heat (H) and drought (D) further reduce plant growth, survival and yield. This study aimed to explore the transcriptional responses of heat shock protein (HSP) and antioxidant genes under H combined D stress in perennial rye grass (PRG). The results demonstrated that oxidative stress indicators (hydrogen peroxide, lipid peroxidation) significantly increased, particularly in the case of combined H and D treatment, suggesting that oxidative stress-induced damage occurred in plants under the combined stresses. Transcriptional responses of heat shock protein 70 (HSP70), heat shock protein 90-6 (HSP90-6), and the mitochondrial small heat shock protein HSP26.2 (HSP26.2) occurred rapidly, and showed high level of expression particularly under H and D stress. Antioxidant genes including ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), catalase (CAT), copper–zinc superoxide dismutase (Cu/ZnSOD), peroxidase (POD), ferredoxin–thioredoxin (FTR), thioredoxin (Trx), 2-cysteine peroxiredoxin (2-Cys Prx) showed response to combined H and D, followed by either D or H stress alone in rye grass. An interactome map revealed the close partnership of these heat shock protein genes and antioxidant genes, respectively. These candidate genes were predominantly linked to stress responses and antioxidant defense in plants. These findings may advance our understanding about the HSP and the antioxidant genes underlying combined abiotic stress response and tolerance in perennial rye grass. MDPI 2022-09-13 /pmc/articles/PMC9506360/ /pubmed/36143461 http://dx.doi.org/10.3390/life12091426 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rahman, Md Atikur
Woo, Jae Hoon
Song, Yowook
Lee, Sang-Hoon
Hasan, Md Mahadi
Azad, Md Abul Kalam
Lee, Ki-Won
Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass
title Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass
title_full Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass
title_fullStr Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass
title_full_unstemmed Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass
title_short Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass
title_sort heat shock proteins and antioxidant genes involved in heat combined with drought stress responses in perennial rye grass
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506360/
https://www.ncbi.nlm.nih.gov/pubmed/36143461
http://dx.doi.org/10.3390/life12091426
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