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Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017

Recent reports of haemagglutinin antigen (HA) mismatch between vaccine composition strains and circulating strains, have led to renewed interest in influenza B viruses. Additionally, there are concerns about resistance to neuraminidase inhibitors in new influenza B isolates. To assess the potential...

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Detalles Bibliográficos
Autores principales: Yakubu, Alhassan Mohammed, Aryee, Nii Ayite, Bonney, Evelyn Yayra, Kotey, Erasmus Nikoi, Bonney, Joseph Humphrey Kofi, Wiley, Michael R., Pratt, Catherine B., Ababio, Grace Korkor, Nimo-Paintsil, Shieley, Puplampu, Naiki, Attoh, Seth, Fatchu, Raymond D., Nyarko, Edward Owusu, Fox, Anne, Watters, Chaselynn M., Sanders, Terrel, Letizia, Andrew G., Ampofo, William Kwabena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506629/
https://www.ncbi.nlm.nih.gov/pubmed/36149889
http://dx.doi.org/10.1371/journal.pone.0271321
Descripción
Sumario:Recent reports of haemagglutinin antigen (HA) mismatch between vaccine composition strains and circulating strains, have led to renewed interest in influenza B viruses. Additionally, there are concerns about resistance to neuraminidase inhibitors in new influenza B isolates. To assess the potential impact in Ghana, we characterized the lineages of influenza B viruses that circulated in Ghana between 2016 and 2017 from different regions of the country: Southern, Northern and Central Ghana. Eight representative specimens from the three regions that were positive for influenza B virus by real-time RT-PCR were sequenced and compared to reference genomes from each lineage. A total of eleven amino acids substitutions were detected in the B/Victoria lineage and six in the B/Yamagata lineage. The strains of influenza B viruses were closely related to influenza B/Brisbane/60/2008 and influenza B/Phuket/3073/2013 for the Victoria and Yamagata lineages, respectively. Three main amino acid substitutions (P31S, I117V and R151K) were found in B/Victoria lineages circulating between 2016 and 2017, while one strain of B/Victoria possessed a unique glycosylation site at amino acid position 51 in the HA2 subunit. Two main substitutions (L172Q and M251V) were detected in the HA gene of the B/Yamagata lineage. The U.S. CDC recently reported a deletion sub-group in influenza B virus, but this was not identified among the Ghanaian specimens. Close monitoring of the patterns of influenza B evolution is necessary for the efficient selection of representative viruses for the design and formulation of effective influenza vaccines.