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Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017

Recent reports of haemagglutinin antigen (HA) mismatch between vaccine composition strains and circulating strains, have led to renewed interest in influenza B viruses. Additionally, there are concerns about resistance to neuraminidase inhibitors in new influenza B isolates. To assess the potential...

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Autores principales: Yakubu, Alhassan Mohammed, Aryee, Nii Ayite, Bonney, Evelyn Yayra, Kotey, Erasmus Nikoi, Bonney, Joseph Humphrey Kofi, Wiley, Michael R., Pratt, Catherine B., Ababio, Grace Korkor, Nimo-Paintsil, Shieley, Puplampu, Naiki, Attoh, Seth, Fatchu, Raymond D., Nyarko, Edward Owusu, Fox, Anne, Watters, Chaselynn M., Sanders, Terrel, Letizia, Andrew G., Ampofo, William Kwabena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506629/
https://www.ncbi.nlm.nih.gov/pubmed/36149889
http://dx.doi.org/10.1371/journal.pone.0271321
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author Yakubu, Alhassan Mohammed
Aryee, Nii Ayite
Bonney, Evelyn Yayra
Kotey, Erasmus Nikoi
Bonney, Joseph Humphrey Kofi
Wiley, Michael R.
Pratt, Catherine B.
Ababio, Grace Korkor
Nimo-Paintsil, Shieley
Puplampu, Naiki
Attoh, Seth
Fatchu, Raymond D.
Nyarko, Edward Owusu
Fox, Anne
Watters, Chaselynn M.
Sanders, Terrel
Letizia, Andrew G.
Ampofo, William Kwabena
author_facet Yakubu, Alhassan Mohammed
Aryee, Nii Ayite
Bonney, Evelyn Yayra
Kotey, Erasmus Nikoi
Bonney, Joseph Humphrey Kofi
Wiley, Michael R.
Pratt, Catherine B.
Ababio, Grace Korkor
Nimo-Paintsil, Shieley
Puplampu, Naiki
Attoh, Seth
Fatchu, Raymond D.
Nyarko, Edward Owusu
Fox, Anne
Watters, Chaselynn M.
Sanders, Terrel
Letizia, Andrew G.
Ampofo, William Kwabena
author_sort Yakubu, Alhassan Mohammed
collection PubMed
description Recent reports of haemagglutinin antigen (HA) mismatch between vaccine composition strains and circulating strains, have led to renewed interest in influenza B viruses. Additionally, there are concerns about resistance to neuraminidase inhibitors in new influenza B isolates. To assess the potential impact in Ghana, we characterized the lineages of influenza B viruses that circulated in Ghana between 2016 and 2017 from different regions of the country: Southern, Northern and Central Ghana. Eight representative specimens from the three regions that were positive for influenza B virus by real-time RT-PCR were sequenced and compared to reference genomes from each lineage. A total of eleven amino acids substitutions were detected in the B/Victoria lineage and six in the B/Yamagata lineage. The strains of influenza B viruses were closely related to influenza B/Brisbane/60/2008 and influenza B/Phuket/3073/2013 for the Victoria and Yamagata lineages, respectively. Three main amino acid substitutions (P31S, I117V and R151K) were found in B/Victoria lineages circulating between 2016 and 2017, while one strain of B/Victoria possessed a unique glycosylation site at amino acid position 51 in the HA2 subunit. Two main substitutions (L172Q and M251V) were detected in the HA gene of the B/Yamagata lineage. The U.S. CDC recently reported a deletion sub-group in influenza B virus, but this was not identified among the Ghanaian specimens. Close monitoring of the patterns of influenza B evolution is necessary for the efficient selection of representative viruses for the design and formulation of effective influenza vaccines.
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spelling pubmed-95066292022-09-24 Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017 Yakubu, Alhassan Mohammed Aryee, Nii Ayite Bonney, Evelyn Yayra Kotey, Erasmus Nikoi Bonney, Joseph Humphrey Kofi Wiley, Michael R. Pratt, Catherine B. Ababio, Grace Korkor Nimo-Paintsil, Shieley Puplampu, Naiki Attoh, Seth Fatchu, Raymond D. Nyarko, Edward Owusu Fox, Anne Watters, Chaselynn M. Sanders, Terrel Letizia, Andrew G. Ampofo, William Kwabena PLoS One Research Article Recent reports of haemagglutinin antigen (HA) mismatch between vaccine composition strains and circulating strains, have led to renewed interest in influenza B viruses. Additionally, there are concerns about resistance to neuraminidase inhibitors in new influenza B isolates. To assess the potential impact in Ghana, we characterized the lineages of influenza B viruses that circulated in Ghana between 2016 and 2017 from different regions of the country: Southern, Northern and Central Ghana. Eight representative specimens from the three regions that were positive for influenza B virus by real-time RT-PCR were sequenced and compared to reference genomes from each lineage. A total of eleven amino acids substitutions were detected in the B/Victoria lineage and six in the B/Yamagata lineage. The strains of influenza B viruses were closely related to influenza B/Brisbane/60/2008 and influenza B/Phuket/3073/2013 for the Victoria and Yamagata lineages, respectively. Three main amino acid substitutions (P31S, I117V and R151K) were found in B/Victoria lineages circulating between 2016 and 2017, while one strain of B/Victoria possessed a unique glycosylation site at amino acid position 51 in the HA2 subunit. Two main substitutions (L172Q and M251V) were detected in the HA gene of the B/Yamagata lineage. The U.S. CDC recently reported a deletion sub-group in influenza B virus, but this was not identified among the Ghanaian specimens. Close monitoring of the patterns of influenza B evolution is necessary for the efficient selection of representative viruses for the design and formulation of effective influenza vaccines. Public Library of Science 2022-09-23 /pmc/articles/PMC9506629/ /pubmed/36149889 http://dx.doi.org/10.1371/journal.pone.0271321 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Yakubu, Alhassan Mohammed
Aryee, Nii Ayite
Bonney, Evelyn Yayra
Kotey, Erasmus Nikoi
Bonney, Joseph Humphrey Kofi
Wiley, Michael R.
Pratt, Catherine B.
Ababio, Grace Korkor
Nimo-Paintsil, Shieley
Puplampu, Naiki
Attoh, Seth
Fatchu, Raymond D.
Nyarko, Edward Owusu
Fox, Anne
Watters, Chaselynn M.
Sanders, Terrel
Letizia, Andrew G.
Ampofo, William Kwabena
Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017
title Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017
title_full Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017
title_fullStr Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017
title_full_unstemmed Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017
title_short Molecular characterization of haemagglutinin genes of influenza B viruses circulating in Ghana during 2016 and 2017
title_sort molecular characterization of haemagglutinin genes of influenza b viruses circulating in ghana during 2016 and 2017
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506629/
https://www.ncbi.nlm.nih.gov/pubmed/36149889
http://dx.doi.org/10.1371/journal.pone.0271321
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