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Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows

Therapeutic drug monitoring is essential for dosing pharmaceuticals with narrow therapeutic windows. Nevertheless, standard methods are imprecise and involve invasive/resource-intensive procedures with long turnaround times. Overcoming these limitations, we present a microneedle-based electrochemica...

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Autores principales: Lin, Shuyu, Cheng, Xuanbing, Zhu, Jialun, Wang, Bo, Jelinek, David, Zhao, Yichao, Wu, Tsung-Yu, Horrillo, Abraham, Tan, Jiawei, Yeung, Justin, Yan, Wenzhong, Forman, Sarah, Coller, Hilary A., Milla, Carlos, Emaminejad, Sam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506728/
https://www.ncbi.nlm.nih.gov/pubmed/36149955
http://dx.doi.org/10.1126/sciadv.abq4539
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author Lin, Shuyu
Cheng, Xuanbing
Zhu, Jialun
Wang, Bo
Jelinek, David
Zhao, Yichao
Wu, Tsung-Yu
Horrillo, Abraham
Tan, Jiawei
Yeung, Justin
Yan, Wenzhong
Forman, Sarah
Coller, Hilary A.
Milla, Carlos
Emaminejad, Sam
author_facet Lin, Shuyu
Cheng, Xuanbing
Zhu, Jialun
Wang, Bo
Jelinek, David
Zhao, Yichao
Wu, Tsung-Yu
Horrillo, Abraham
Tan, Jiawei
Yeung, Justin
Yan, Wenzhong
Forman, Sarah
Coller, Hilary A.
Milla, Carlos
Emaminejad, Sam
author_sort Lin, Shuyu
collection PubMed
description Therapeutic drug monitoring is essential for dosing pharmaceuticals with narrow therapeutic windows. Nevertheless, standard methods are imprecise and involve invasive/resource-intensive procedures with long turnaround times. Overcoming these limitations, we present a microneedle-based electrochemical aptamer biosensing patch (μNEAB-patch) that minimally invasively probes the interstitial fluid (ISF) and renders correlated, continuous, and real-time measurements of the circulating drugs’ pharmacokinetics. The μNEAB-patch is created following an introduced low-cost fabrication scheme, which transforms a shortened clinical-grade needle into a high-quality gold nanoparticle-based substrate for robust aptamer immobilization and efficient electrochemical signal retrieval. This enables the reliable in vivo detection of a wide library of ISF analytes—especially those with nonexistent natural recognition elements. Accordingly, we developed μNEABs targeting various drugs, including antibiotics with narrow therapeutic windows (tobramycin and vancomycin). Through in vivo animal studies, we demonstrated the strong correlation between the ISF/circulating drug levels and the device’s potential clinical use for timely prediction of total drug exposure.
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spelling pubmed-95067282022-10-07 Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows Lin, Shuyu Cheng, Xuanbing Zhu, Jialun Wang, Bo Jelinek, David Zhao, Yichao Wu, Tsung-Yu Horrillo, Abraham Tan, Jiawei Yeung, Justin Yan, Wenzhong Forman, Sarah Coller, Hilary A. Milla, Carlos Emaminejad, Sam Sci Adv Physical and Materials Sciences Therapeutic drug monitoring is essential for dosing pharmaceuticals with narrow therapeutic windows. Nevertheless, standard methods are imprecise and involve invasive/resource-intensive procedures with long turnaround times. Overcoming these limitations, we present a microneedle-based electrochemical aptamer biosensing patch (μNEAB-patch) that minimally invasively probes the interstitial fluid (ISF) and renders correlated, continuous, and real-time measurements of the circulating drugs’ pharmacokinetics. The μNEAB-patch is created following an introduced low-cost fabrication scheme, which transforms a shortened clinical-grade needle into a high-quality gold nanoparticle-based substrate for robust aptamer immobilization and efficient electrochemical signal retrieval. This enables the reliable in vivo detection of a wide library of ISF analytes—especially those with nonexistent natural recognition elements. Accordingly, we developed μNEABs targeting various drugs, including antibiotics with narrow therapeutic windows (tobramycin and vancomycin). Through in vivo animal studies, we demonstrated the strong correlation between the ISF/circulating drug levels and the device’s potential clinical use for timely prediction of total drug exposure. American Association for the Advancement of Science 2022-09-23 /pmc/articles/PMC9506728/ /pubmed/36149955 http://dx.doi.org/10.1126/sciadv.abq4539 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Physical and Materials Sciences
Lin, Shuyu
Cheng, Xuanbing
Zhu, Jialun
Wang, Bo
Jelinek, David
Zhao, Yichao
Wu, Tsung-Yu
Horrillo, Abraham
Tan, Jiawei
Yeung, Justin
Yan, Wenzhong
Forman, Sarah
Coller, Hilary A.
Milla, Carlos
Emaminejad, Sam
Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows
title Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows
title_full Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows
title_fullStr Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows
title_full_unstemmed Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows
title_short Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows
title_sort wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows
topic Physical and Materials Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506728/
https://www.ncbi.nlm.nih.gov/pubmed/36149955
http://dx.doi.org/10.1126/sciadv.abq4539
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