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Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows
Therapeutic drug monitoring is essential for dosing pharmaceuticals with narrow therapeutic windows. Nevertheless, standard methods are imprecise and involve invasive/resource-intensive procedures with long turnaround times. Overcoming these limitations, we present a microneedle-based electrochemica...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506728/ https://www.ncbi.nlm.nih.gov/pubmed/36149955 http://dx.doi.org/10.1126/sciadv.abq4539 |
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author | Lin, Shuyu Cheng, Xuanbing Zhu, Jialun Wang, Bo Jelinek, David Zhao, Yichao Wu, Tsung-Yu Horrillo, Abraham Tan, Jiawei Yeung, Justin Yan, Wenzhong Forman, Sarah Coller, Hilary A. Milla, Carlos Emaminejad, Sam |
author_facet | Lin, Shuyu Cheng, Xuanbing Zhu, Jialun Wang, Bo Jelinek, David Zhao, Yichao Wu, Tsung-Yu Horrillo, Abraham Tan, Jiawei Yeung, Justin Yan, Wenzhong Forman, Sarah Coller, Hilary A. Milla, Carlos Emaminejad, Sam |
author_sort | Lin, Shuyu |
collection | PubMed |
description | Therapeutic drug monitoring is essential for dosing pharmaceuticals with narrow therapeutic windows. Nevertheless, standard methods are imprecise and involve invasive/resource-intensive procedures with long turnaround times. Overcoming these limitations, we present a microneedle-based electrochemical aptamer biosensing patch (μNEAB-patch) that minimally invasively probes the interstitial fluid (ISF) and renders correlated, continuous, and real-time measurements of the circulating drugs’ pharmacokinetics. The μNEAB-patch is created following an introduced low-cost fabrication scheme, which transforms a shortened clinical-grade needle into a high-quality gold nanoparticle-based substrate for robust aptamer immobilization and efficient electrochemical signal retrieval. This enables the reliable in vivo detection of a wide library of ISF analytes—especially those with nonexistent natural recognition elements. Accordingly, we developed μNEABs targeting various drugs, including antibiotics with narrow therapeutic windows (tobramycin and vancomycin). Through in vivo animal studies, we demonstrated the strong correlation between the ISF/circulating drug levels and the device’s potential clinical use for timely prediction of total drug exposure. |
format | Online Article Text |
id | pubmed-9506728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-95067282022-10-07 Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows Lin, Shuyu Cheng, Xuanbing Zhu, Jialun Wang, Bo Jelinek, David Zhao, Yichao Wu, Tsung-Yu Horrillo, Abraham Tan, Jiawei Yeung, Justin Yan, Wenzhong Forman, Sarah Coller, Hilary A. Milla, Carlos Emaminejad, Sam Sci Adv Physical and Materials Sciences Therapeutic drug monitoring is essential for dosing pharmaceuticals with narrow therapeutic windows. Nevertheless, standard methods are imprecise and involve invasive/resource-intensive procedures with long turnaround times. Overcoming these limitations, we present a microneedle-based electrochemical aptamer biosensing patch (μNEAB-patch) that minimally invasively probes the interstitial fluid (ISF) and renders correlated, continuous, and real-time measurements of the circulating drugs’ pharmacokinetics. The μNEAB-patch is created following an introduced low-cost fabrication scheme, which transforms a shortened clinical-grade needle into a high-quality gold nanoparticle-based substrate for robust aptamer immobilization and efficient electrochemical signal retrieval. This enables the reliable in vivo detection of a wide library of ISF analytes—especially those with nonexistent natural recognition elements. Accordingly, we developed μNEABs targeting various drugs, including antibiotics with narrow therapeutic windows (tobramycin and vancomycin). Through in vivo animal studies, we demonstrated the strong correlation between the ISF/circulating drug levels and the device’s potential clinical use for timely prediction of total drug exposure. American Association for the Advancement of Science 2022-09-23 /pmc/articles/PMC9506728/ /pubmed/36149955 http://dx.doi.org/10.1126/sciadv.abq4539 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Physical and Materials Sciences Lin, Shuyu Cheng, Xuanbing Zhu, Jialun Wang, Bo Jelinek, David Zhao, Yichao Wu, Tsung-Yu Horrillo, Abraham Tan, Jiawei Yeung, Justin Yan, Wenzhong Forman, Sarah Coller, Hilary A. Milla, Carlos Emaminejad, Sam Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows |
title | Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows |
title_full | Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows |
title_fullStr | Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows |
title_full_unstemmed | Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows |
title_short | Wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows |
title_sort | wearable microneedle-based electrochemical aptamer biosensing for precision dosing of drugs with narrow therapeutic windows |
topic | Physical and Materials Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506728/ https://www.ncbi.nlm.nih.gov/pubmed/36149955 http://dx.doi.org/10.1126/sciadv.abq4539 |
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