Cargando…

Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane

Salmonella enteritica (S. enteritica) induce and require unfolded protein response (UPR) pathways for intracellular replication. Salmonella infections can lead to reactive arthritis (ReA), which can exhibit associations with Human Leucocyte Antigen (HLA)-B(∗)27 : 05. S. enteritica normally reside in...

Descripción completa

Detalles Bibliográficos
Autores principales: Kriston-Vizi, Janos, Lenart, Izabela, Iwawaki, Takao, Gould, Keith, Nesbeth, Darren, Powis, Simon J., Antoniou, Antony N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9507774/
https://www.ncbi.nlm.nih.gov/pubmed/36157878
http://dx.doi.org/10.1155/2022/9493019
_version_ 1784796908063031296
author Kriston-Vizi, Janos
Lenart, Izabela
Iwawaki, Takao
Gould, Keith
Nesbeth, Darren
Powis, Simon J.
Antoniou, Antony N.
author_facet Kriston-Vizi, Janos
Lenart, Izabela
Iwawaki, Takao
Gould, Keith
Nesbeth, Darren
Powis, Simon J.
Antoniou, Antony N.
author_sort Kriston-Vizi, Janos
collection PubMed
description Salmonella enteritica (S. enteritica) induce and require unfolded protein response (UPR) pathways for intracellular replication. Salmonella infections can lead to reactive arthritis (ReA), which can exhibit associations with Human Leucocyte Antigen (HLA)-B(∗)27 : 05. S. enteritica normally reside in a juxtanuclear position to the Golgi apparatus, representing the formation and residence within the Salmonella-containing vacuole (SCV). Changes in cellular localization of infecting Salmonella can alter their ability to replicate. We therefore used isogenic epithelial cell lines expressing physiological levels of HLA-B(∗)27 : 05 heavy chain (HC) and a control HLA-B allele, HLA-B(∗)35 : 01.HC to determine any changes in Salmonella localization within epithelial cells. Expression of HLA-B(∗)27 : 05 but not HLA-B(∗)35 : 01 was associated with a quantifiable change in S. enteritica cellular distribution away from the Golgi apparatus. Furthermore, the Salmonella requirements for UPR induction and the consequences of the concomitant endoplasmic reticulum (ER) membrane expansion were determined. Using confocal imaging, S. enteritica bacteria exhibited a significant and quantifiable codistribution with endo-reticular membrane as determined by ER tracker staining. Isogenic S. enterica Typhimurium mutant strains, which can infect but exhibit impaired intracellular growth, demonstrated that the activation of the UPR was dependent on an integral intracellular niche. Therefore, these data identify cellular changes accompanying Salmonella induction of the UPR and in the presence of HLA-B27.
format Online
Article
Text
id pubmed-9507774
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-95077742022-09-24 Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane Kriston-Vizi, Janos Lenart, Izabela Iwawaki, Takao Gould, Keith Nesbeth, Darren Powis, Simon J. Antoniou, Antony N. J Immunol Res Research Article Salmonella enteritica (S. enteritica) induce and require unfolded protein response (UPR) pathways for intracellular replication. Salmonella infections can lead to reactive arthritis (ReA), which can exhibit associations with Human Leucocyte Antigen (HLA)-B(∗)27 : 05. S. enteritica normally reside in a juxtanuclear position to the Golgi apparatus, representing the formation and residence within the Salmonella-containing vacuole (SCV). Changes in cellular localization of infecting Salmonella can alter their ability to replicate. We therefore used isogenic epithelial cell lines expressing physiological levels of HLA-B(∗)27 : 05 heavy chain (HC) and a control HLA-B allele, HLA-B(∗)35 : 01.HC to determine any changes in Salmonella localization within epithelial cells. Expression of HLA-B(∗)27 : 05 but not HLA-B(∗)35 : 01 was associated with a quantifiable change in S. enteritica cellular distribution away from the Golgi apparatus. Furthermore, the Salmonella requirements for UPR induction and the consequences of the concomitant endoplasmic reticulum (ER) membrane expansion were determined. Using confocal imaging, S. enteritica bacteria exhibited a significant and quantifiable codistribution with endo-reticular membrane as determined by ER tracker staining. Isogenic S. enterica Typhimurium mutant strains, which can infect but exhibit impaired intracellular growth, demonstrated that the activation of the UPR was dependent on an integral intracellular niche. Therefore, these data identify cellular changes accompanying Salmonella induction of the UPR and in the presence of HLA-B27. Hindawi 2022-09-16 /pmc/articles/PMC9507774/ /pubmed/36157878 http://dx.doi.org/10.1155/2022/9493019 Text en Copyright © 2022 Janos Kriston-Vizi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kriston-Vizi, Janos
Lenart, Izabela
Iwawaki, Takao
Gould, Keith
Nesbeth, Darren
Powis, Simon J.
Antoniou, Antony N.
Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane
title Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane
title_full Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane
title_fullStr Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane
title_full_unstemmed Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane
title_short Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane
title_sort salmonella exhibit altered cellular localization in the presence of hla-b27 and codistribute with endo-reticular membrane
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9507774/
https://www.ncbi.nlm.nih.gov/pubmed/36157878
http://dx.doi.org/10.1155/2022/9493019
work_keys_str_mv AT kristonvizijanos salmonellaexhibitalteredcellularlocalizationinthepresenceofhlab27andcodistributewithendoreticularmembrane
AT lenartizabela salmonellaexhibitalteredcellularlocalizationinthepresenceofhlab27andcodistributewithendoreticularmembrane
AT iwawakitakao salmonellaexhibitalteredcellularlocalizationinthepresenceofhlab27andcodistributewithendoreticularmembrane
AT gouldkeith salmonellaexhibitalteredcellularlocalizationinthepresenceofhlab27andcodistributewithendoreticularmembrane
AT nesbethdarren salmonellaexhibitalteredcellularlocalizationinthepresenceofhlab27andcodistributewithendoreticularmembrane
AT powissimonj salmonellaexhibitalteredcellularlocalizationinthepresenceofhlab27andcodistributewithendoreticularmembrane
AT antoniouantonyn salmonellaexhibitalteredcellularlocalizationinthepresenceofhlab27andcodistributewithendoreticularmembrane