Differentiating functional human islet-like aggregates from pluripotent stem cells

We present here a robust and reliable protocol by which to differentiate pancreatic islet-like aggregates (SC-islets) from human pluripotent stem cells. The 7-stage protocol mimics developmental patterning factors that induce endocrine lineage formation and spans monolayer, microwell, and aggregate...

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Detalles Bibliográficos
Autores principales: Barsby, Tom, Ibrahim, Hazem, Lithovius, Väinö, Montaser, Hossam, Balboa, Diego, Vähäkangas, Eliisa, Chandra, Vikash, Saarimäki-Vire, Jonna, Otonkoski, Timo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508476/
https://www.ncbi.nlm.nih.gov/pubmed/36136756
http://dx.doi.org/10.1016/j.xpro.2022.101711
Descripción
Sumario:We present here a robust and reliable protocol by which to differentiate pancreatic islet-like aggregates (SC-islets) from human pluripotent stem cells. The 7-stage protocol mimics developmental patterning factors that induce endocrine lineage formation and spans monolayer, microwell, and aggregate suspension culture. The SC-islets demonstrate dynamic glucose-sensitive insulin secretion and an endocrine cell composition similar to those of primary human islets. SC-islets generated using this optimized protocol are suitable for in vitro modeling of islet cell pathophysiology and therapeutic applications. For complete details on the use and execution of this protocol, please refer to Balboa et al. (2022).

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