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Differentiating functional human islet-like aggregates from pluripotent stem cells

We present here a robust and reliable protocol by which to differentiate pancreatic islet-like aggregates (SC-islets) from human pluripotent stem cells. The 7-stage protocol mimics developmental patterning factors that induce endocrine lineage formation and spans monolayer, microwell, and aggregate...

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Detalles Bibliográficos
Autores principales:	Barsby, Tom, Ibrahim, Hazem, Lithovius, Väinö, Montaser, Hossam, Balboa, Diego, Vähäkangas, Eliisa, Chandra, Vikash, Saarimäki-Vire, Jonna, Otonkoski, Timo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508476/ https://www.ncbi.nlm.nih.gov/pubmed/36136756 http://dx.doi.org/10.1016/j.xpro.2022.101711

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