Angiogenic potential of Bambusa vulgaris leaves: Results of an in-vitro study with chicken embryo chorioallantoic membrane (CAM) model

BACKGROUND: Herbal compounds have an important place in the management of wounds. However, very few compounds have been tested for their proangiogenic potential. OBJECTIVE: The objective of study was to evaluate angiogenic potential of Bambusa vulgaris Schrad. plant extract. MATERIAL AND METHODS: Chicken embryo chorioallantoic membrane model was used for estimation of angiogenic potential of B. vulgaris leaves extract with different concentrations. Angiogenic potential was estimated using focal application method and AbGel™ sponge application method. The test samples were loaded on eight embryonic development day of embryonic development of chick embryo and angiogenesis was observed on eleventh embryonic development day. Counting of blood vessels and photographic evaluation was done for estimation of angiogenic potential. The sponge specimen was examined for histological changes. Angiogenic potential of B. vulgaris leaves extract was compared against Plermin©. RESULTS: All tested concentrations (85 mcg, 170 mcg, 255 mcg, 340 mcg and 425 mcg/disc) of B. vulgaris showed angiogenic potential as indicated by increase in the number of blood vessels. Maximum growth in blood vessels was seen at the concentration of 255 mcg. Photographic evaluation showed changes in angiogenesis with B. vulgaris leaves extract. Angiogenic potential was also confirmed on histological examination. Plermin control groups also showed the growth of blood vessels measured by counting the number of blood vessels in photographic evaluation. The growth of blood vessels with Plermin 40 mcg was similar to B. vulgaris 255 mcg. CONCLUSION: The results of current study suggest angiogenic potential of B. vulgaris Schrad leaves as confirmed by visual observation and histological examination.