Cargando…

A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use

Candida glabrata is an important pathogen causing superficial to invasive disease in human. Conditional expression systems are helpful in addressing the function of genes and especially when they can be applied to in vivo studies. Tetracycline-dependent regulation systems have been used in diverse f...

Descripción completa

Detalles Bibliográficos
Autores principales: Schrevens, S, Sanglard, D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508828/
https://www.ncbi.nlm.nih.gov/pubmed/36047937
http://dx.doi.org/10.1093/femsyr/foac046
_version_ 1784797103271182336
author Schrevens, S
Sanglard, D
author_facet Schrevens, S
Sanglard, D
author_sort Schrevens, S
collection PubMed
description Candida glabrata is an important pathogen causing superficial to invasive disease in human. Conditional expression systems are helpful in addressing the function of genes and especially when they can be applied to in vivo studies. Tetracycline-dependent regulation systems have been used in diverse fungi to turn-on (Tet-on) or turn-off (Tet-off) gene expression either in vitro but also in vivo in animal models. Up to now, only a Tet-off expression has been constructed for gene expression in C. glabrata. Here, we report a Tet-on gene expression system which can be used in vitro and in vivo in any C. glabrata genetic background. This system was used in a mice model of systemic infection to demonstrate that the general amino acid permease Gap1 is important for C. glabrata virulence.
format Online
Article
Text
id pubmed-9508828
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-95088282022-09-26 A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use Schrevens, S Sanglard, D FEMS Yeast Res Research Article Candida glabrata is an important pathogen causing superficial to invasive disease in human. Conditional expression systems are helpful in addressing the function of genes and especially when they can be applied to in vivo studies. Tetracycline-dependent regulation systems have been used in diverse fungi to turn-on (Tet-on) or turn-off (Tet-off) gene expression either in vitro but also in vivo in animal models. Up to now, only a Tet-off expression has been constructed for gene expression in C. glabrata. Here, we report a Tet-on gene expression system which can be used in vitro and in vivo in any C. glabrata genetic background. This system was used in a mice model of systemic infection to demonstrate that the general amino acid permease Gap1 is important for C. glabrata virulence. Oxford University Press 2022-09-01 /pmc/articles/PMC9508828/ /pubmed/36047937 http://dx.doi.org/10.1093/femsyr/foac046 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Schrevens, S
Sanglard, D
A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use
title A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use
title_full A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use
title_fullStr A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use
title_full_unstemmed A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use
title_short A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use
title_sort novel candida glabrata doxycycline-inducible system for in vitro/in vivo use
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508828/
https://www.ncbi.nlm.nih.gov/pubmed/36047937
http://dx.doi.org/10.1093/femsyr/foac046
work_keys_str_mv AT schrevenss anovelcandidaglabratadoxycyclineinduciblesystemforinvitroinvivouse
AT sanglardd anovelcandidaglabratadoxycyclineinduciblesystemforinvitroinvivouse
AT schrevenss novelcandidaglabratadoxycyclineinduciblesystemforinvitroinvivouse
AT sanglardd novelcandidaglabratadoxycyclineinduciblesystemforinvitroinvivouse