Cargando…
CuO-TiO(2)-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line
In this study, cells from human Chronic Myelogenous Leukemia (K562) were cultivated with CuO-TiO(2)-Chitosan-Berbamine nanocomposites. We examined nanocomposites using XRD, DLS, FESEM, TEM, PL, EDAX, and FTIR spectroscopy, as well as MTT for cytotoxicity, and AO/EtBr for apoptotic morphology assessm...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9509271/ https://www.ncbi.nlm.nih.gov/pubmed/36164585 http://dx.doi.org/10.1155/2022/9602725 |
_version_ | 1784797201159946240 |
---|---|
author | Elderdery, Abozer Y. Alzahrani, Badr Hamza, Siddiqa M. A Mostafa-Hedeab, Gomaa Mok, Pooi Ling Subbiah, Suresh Kumar |
author_facet | Elderdery, Abozer Y. Alzahrani, Badr Hamza, Siddiqa M. A Mostafa-Hedeab, Gomaa Mok, Pooi Ling Subbiah, Suresh Kumar |
author_sort | Elderdery, Abozer Y. |
collection | PubMed |
description | In this study, cells from human Chronic Myelogenous Leukemia (K562) were cultivated with CuO-TiO(2)-Chitosan-Berbamine nanocomposites. We examined nanocomposites using XRD, DLS, FESEM, TEM, PL, EDAX, and FTIR spectroscopy, as well as MTT for cytotoxicity, and AO/EtBr for apoptotic morphology assessment. The rate of apoptosis and cell cycle arrests was determined using flow cytometry. Flow cytometry was also employed to identify pro- and antiapoptotic proteins such as Bcl2, Bad, Bax, P53, and Cyt C. The FTIR spectrum revealed that the CuO-TiO(2)-Chitosan-Berbamine nanocomposites were electrostatically interlocked. The nanocomposites' XRD signals revealed a hexagonal shape. In the DLS spectrum, nanocomposites were found to have a hydrodynamic diameter. As a result of their cytotoxic action, nanocomposites displayed concentration-dependent cytotoxicity. The nanocomposites, like Doxorubicin, caused cell cycle phase arrest in K562 cells. After treatment with IC(50) concentrations of CuO-TiO(2)-Chitosan-Berbamine nanocomposites and Doxorubicin, a substantial percentage of cells were in G2/M stage arrest. Caspase-3, -7, -8, -9, Bax, Bad, Cyt C, and P53 expression were considerably enhanced in K562 cells, whereas Bcl2 expression was decreased, indicating that these cells may have therapeutic potential against human blood cancer/leukemia-derived disorders. As a result, the nanocomposites demonstrated outstanding anticancer potential against leukemic cells. CuO-TiO(2)-Chitosan-Berbamine, according to our findings. |
format | Online Article Text |
id | pubmed-9509271 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95092712022-09-25 CuO-TiO(2)-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line Elderdery, Abozer Y. Alzahrani, Badr Hamza, Siddiqa M. A Mostafa-Hedeab, Gomaa Mok, Pooi Ling Subbiah, Suresh Kumar Bioinorg Chem Appl Research Article In this study, cells from human Chronic Myelogenous Leukemia (K562) were cultivated with CuO-TiO(2)-Chitosan-Berbamine nanocomposites. We examined nanocomposites using XRD, DLS, FESEM, TEM, PL, EDAX, and FTIR spectroscopy, as well as MTT for cytotoxicity, and AO/EtBr for apoptotic morphology assessment. The rate of apoptosis and cell cycle arrests was determined using flow cytometry. Flow cytometry was also employed to identify pro- and antiapoptotic proteins such as Bcl2, Bad, Bax, P53, and Cyt C. The FTIR spectrum revealed that the CuO-TiO(2)-Chitosan-Berbamine nanocomposites were electrostatically interlocked. The nanocomposites' XRD signals revealed a hexagonal shape. In the DLS spectrum, nanocomposites were found to have a hydrodynamic diameter. As a result of their cytotoxic action, nanocomposites displayed concentration-dependent cytotoxicity. The nanocomposites, like Doxorubicin, caused cell cycle phase arrest in K562 cells. After treatment with IC(50) concentrations of CuO-TiO(2)-Chitosan-Berbamine nanocomposites and Doxorubicin, a substantial percentage of cells were in G2/M stage arrest. Caspase-3, -7, -8, -9, Bax, Bad, Cyt C, and P53 expression were considerably enhanced in K562 cells, whereas Bcl2 expression was decreased, indicating that these cells may have therapeutic potential against human blood cancer/leukemia-derived disorders. As a result, the nanocomposites demonstrated outstanding anticancer potential against leukemic cells. CuO-TiO(2)-Chitosan-Berbamine, according to our findings. Hindawi 2022-09-17 /pmc/articles/PMC9509271/ /pubmed/36164585 http://dx.doi.org/10.1155/2022/9602725 Text en Copyright © 2022 Abozer Y. Elderdery et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Elderdery, Abozer Y. Alzahrani, Badr Hamza, Siddiqa M. A Mostafa-Hedeab, Gomaa Mok, Pooi Ling Subbiah, Suresh Kumar CuO-TiO(2)-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line |
title | CuO-TiO(2)-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line |
title_full | CuO-TiO(2)-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line |
title_fullStr | CuO-TiO(2)-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line |
title_full_unstemmed | CuO-TiO(2)-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line |
title_short | CuO-TiO(2)-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line |
title_sort | cuo-tio(2)-chitosan-berbamine nanocomposites induce apoptosis through the mitochondrial pathway with the expression of p53, bax, and bcl-2 in the human k562 cancer cell line |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9509271/ https://www.ncbi.nlm.nih.gov/pubmed/36164585 http://dx.doi.org/10.1155/2022/9602725 |
work_keys_str_mv | AT elderderyabozery cuotio2chitosanberbaminenanocompositesinduceapoptosisthroughthemitochondrialpathwaywiththeexpressionofp53baxandbcl2inthehumank562cancercellline AT alzahranibadr cuotio2chitosanberbaminenanocompositesinduceapoptosisthroughthemitochondrialpathwaywiththeexpressionofp53baxandbcl2inthehumank562cancercellline AT hamzasiddiqama cuotio2chitosanberbaminenanocompositesinduceapoptosisthroughthemitochondrialpathwaywiththeexpressionofp53baxandbcl2inthehumank562cancercellline AT mostafahedeabgomaa cuotio2chitosanberbaminenanocompositesinduceapoptosisthroughthemitochondrialpathwaywiththeexpressionofp53baxandbcl2inthehumank562cancercellline AT mokpooiling cuotio2chitosanberbaminenanocompositesinduceapoptosisthroughthemitochondrialpathwaywiththeexpressionofp53baxandbcl2inthehumank562cancercellline AT subbiahsureshkumar cuotio2chitosanberbaminenanocompositesinduceapoptosisthroughthemitochondrialpathwaywiththeexpressionofp53baxandbcl2inthehumank562cancercellline |