Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation

OBJECTIVE: Engineering bone in 3D is important for both regenerative medicine purposes and for the development of accurate in vitro models of bone tissue. The changing material stiffness of bone tissue had not yet been monitored throughout the process of mineralisation and bone nodule formation by o...

Descripción completa

Detalles Bibliográficos
Autores principales: Bakkalci, Deniz, Micalet, Auxtine, Al Hosni, Rawiya, Moeendarbary, Emad, Cheema, Umber
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9509582/
https://www.ncbi.nlm.nih.gov/pubmed/36153566
http://dx.doi.org/10.1186/s13104-022-06203-z
_version_ 1784797257146564608
author Bakkalci, Deniz
Micalet, Auxtine
Al Hosni, Rawiya
Moeendarbary, Emad
Cheema, Umber
author_facet Bakkalci, Deniz
Micalet, Auxtine
Al Hosni, Rawiya
Moeendarbary, Emad
Cheema, Umber
author_sort Bakkalci, Deniz
collection PubMed
description OBJECTIVE: Engineering bone in 3D is important for both regenerative medicine purposes and for the development of accurate in vitro models of bone tissue. The changing material stiffness of bone tissue had not yet been monitored throughout the process of mineralisation and bone nodule formation by osteoblasts either during in vitro engineering or in development perspective. RESULTS: Within this short research note, stiffness changes (Young’s modulus) during in vitro bone formation by primary osteoblasts in dense collagen scaffolds were monitored using atomic force microscopy. Data analysis revealed significant stiffening of 3D bone cultures at day 5 and 8 that was correlated with the onset of mineral deposition (p < 0.00005).
format Online
Article
Text
id pubmed-9509582
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-95095822022-09-26 Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation Bakkalci, Deniz Micalet, Auxtine Al Hosni, Rawiya Moeendarbary, Emad Cheema, Umber BMC Res Notes Research Note OBJECTIVE: Engineering bone in 3D is important for both regenerative medicine purposes and for the development of accurate in vitro models of bone tissue. The changing material stiffness of bone tissue had not yet been monitored throughout the process of mineralisation and bone nodule formation by osteoblasts either during in vitro engineering or in development perspective. RESULTS: Within this short research note, stiffness changes (Young’s modulus) during in vitro bone formation by primary osteoblasts in dense collagen scaffolds were monitored using atomic force microscopy. Data analysis revealed significant stiffening of 3D bone cultures at day 5 and 8 that was correlated with the onset of mineral deposition (p < 0.00005). BioMed Central 2022-09-24 /pmc/articles/PMC9509582/ /pubmed/36153566 http://dx.doi.org/10.1186/s13104-022-06203-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Bakkalci, Deniz
Micalet, Auxtine
Al Hosni, Rawiya
Moeendarbary, Emad
Cheema, Umber
Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation
title Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation
title_full Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation
title_fullStr Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation
title_full_unstemmed Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation
title_short Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation
title_sort associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9509582/
https://www.ncbi.nlm.nih.gov/pubmed/36153566
http://dx.doi.org/10.1186/s13104-022-06203-z
work_keys_str_mv AT bakkalcideniz associatedchangesinstiffnessofcollagenscaffoldsduringosteoblastmineralisationandboneformation
AT micaletauxtine associatedchangesinstiffnessofcollagenscaffoldsduringosteoblastmineralisationandboneformation
AT alhosnirawiya associatedchangesinstiffnessofcollagenscaffoldsduringosteoblastmineralisationandboneformation
AT moeendarbaryemad associatedchangesinstiffnessofcollagenscaffoldsduringosteoblastmineralisationandboneformation
AT cheemaumber associatedchangesinstiffnessofcollagenscaffoldsduringosteoblastmineralisationandboneformation

Ejemplares similares