P013 Green synthesis of silver nanoparticles using Trillium govanianum and its antifungal potential against Candida auris

POSTER SESSION 1, SEPTEMBER 21, 2022, 12:30 PM - 1:30 PM: OBJECTIVE: This study aimed to evaluate the antifungal and anti-biofilm activities of silver nanoparticles synthesized using Trillium govanianum, a trans-Himalayan medicinal plant extract, against multidrug-resistant Candida auris. METHODS: Silver nanoparticles (AgNPs) were synthesized by adopting a green approach. Optimization and characterization of TG-AgNPs were carried out using UV-VIS spectroscopy, TEM, and FTIR. Minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), and antibiofilm activity of the crude extract, as well as TG-nanoparticles, were evaluated. Furthermore, their effect on cell morphology and cell permeability was also analyzed using FESEM. RESULTS: Crude extracts of roots of T. govanianum exerted remarkable antifungal activity against multiple strains of Candida auris. The incorporation of silver nanoparticles to plant extract significantly improved the antifungal and antibiofilm activities against the tested strains in a synergistic manner. CONCLUSION: Silver nanoparticles synthesized using Trillium govanianum extract provide eco-friendly, biocompatible nanostructures with antifungal activity and have the potential for use as a therapeutic/nutraceutical agent.