Cargando…
P351 Genetic recombination between the main human skin commensal fungus, Malassezia restricta, and M. globosa using an Agrobacterium tumefaciens-mediated gene transfer system
POSTER SESSION 3, SEPTEMBER 23, 2022, 12:30 PM - 1:30 PM: OBJECTS: Malassezia restricta and M. globosa cause or exacerbate Malassezia -associated skin, seborrheic, and atopic dermatitis, as well as pityriasis versicolor, but the virulence factors remain unclear because between-species genetic reco...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9509928/ http://dx.doi.org/10.1093/mmy/myac072.P351 |
_version_ | 1784797337214779392 |
---|---|
author | Cho, Otomi Matsumoto, Yasuhiko Yamada, Tsuyoshi Sugita, Takashi |
author_facet | Cho, Otomi Matsumoto, Yasuhiko Yamada, Tsuyoshi Sugita, Takashi |
author_sort | Cho, Otomi |
collection | PubMed |
description | POSTER SESSION 3, SEPTEMBER 23, 2022, 12:30 PM - 1:30 PM: OBJECTS: Malassezia restricta and M. globosa cause or exacerbate Malassezia -associated skin, seborrheic, and atopic dermatitis, as well as pityriasis versicolor, but the virulence factors remain unclear because between-species genetic recombination has not yet been achieved. We built an Agrobacterium tumefaciens-mediated gene transfer (ATMT) system that generated gene-knockout mutants of both Malassezia species. MATERIALS AND METHODS: The binary vector pAg1-Δfkb1::NAT1 was introduced into M. restricta CBS 7877 and M. globosa CBS 7966 via ATMT; FKB1 was replaced. Then, FKB1 was re-introduced into the mutants to counteract the deficiencies. RESULTS AND DISCUSSION: The medium acetosyringone concentration and temperature, as well as the co-cultivation ratios of A. tumefaciens and the Malassezia strains, affected recombination efficacy. We generated a mutant of the FKB1 gene (which encodes the FKBP12 protein that binds to the calcineurin inhibitor tacrolimus). Wild-type M. restricta and M. globosa were tacrolimus-sensitive, while the FKB1 mutants were tacrolimus-resistant; drug susceptibility was restored by reintroducing FKB1. CONCLUSION: Studies on recombination between M. restricta and M. globosa will aid elucidation of the molecular mechanisms underlying Malassezia -associated dermatitis. |
format | Online Article Text |
id | pubmed-9509928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-95099282022-09-26 P351 Genetic recombination between the main human skin commensal fungus, Malassezia restricta, and M. globosa using an Agrobacterium tumefaciens-mediated gene transfer system Cho, Otomi Matsumoto, Yasuhiko Yamada, Tsuyoshi Sugita, Takashi Med Mycol Oral Presentations POSTER SESSION 3, SEPTEMBER 23, 2022, 12:30 PM - 1:30 PM: OBJECTS: Malassezia restricta and M. globosa cause or exacerbate Malassezia -associated skin, seborrheic, and atopic dermatitis, as well as pityriasis versicolor, but the virulence factors remain unclear because between-species genetic recombination has not yet been achieved. We built an Agrobacterium tumefaciens-mediated gene transfer (ATMT) system that generated gene-knockout mutants of both Malassezia species. MATERIALS AND METHODS: The binary vector pAg1-Δfkb1::NAT1 was introduced into M. restricta CBS 7877 and M. globosa CBS 7966 via ATMT; FKB1 was replaced. Then, FKB1 was re-introduced into the mutants to counteract the deficiencies. RESULTS AND DISCUSSION: The medium acetosyringone concentration and temperature, as well as the co-cultivation ratios of A. tumefaciens and the Malassezia strains, affected recombination efficacy. We generated a mutant of the FKB1 gene (which encodes the FKBP12 protein that binds to the calcineurin inhibitor tacrolimus). Wild-type M. restricta and M. globosa were tacrolimus-sensitive, while the FKB1 mutants were tacrolimus-resistant; drug susceptibility was restored by reintroducing FKB1. CONCLUSION: Studies on recombination between M. restricta and M. globosa will aid elucidation of the molecular mechanisms underlying Malassezia -associated dermatitis. Oxford University Press 2022-09-20 /pmc/articles/PMC9509928/ http://dx.doi.org/10.1093/mmy/myac072.P351 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Oral Presentations Cho, Otomi Matsumoto, Yasuhiko Yamada, Tsuyoshi Sugita, Takashi P351 Genetic recombination between the main human skin commensal fungus, Malassezia restricta, and M. globosa using an Agrobacterium tumefaciens-mediated gene transfer system |
title | P351 Genetic recombination between the main human skin commensal fungus, Malassezia restricta, and M. globosa using an Agrobacterium tumefaciens-mediated gene transfer system |
title_full | P351 Genetic recombination between the main human skin commensal fungus, Malassezia restricta, and M. globosa using an Agrobacterium tumefaciens-mediated gene transfer system |
title_fullStr | P351 Genetic recombination between the main human skin commensal fungus, Malassezia restricta, and M. globosa using an Agrobacterium tumefaciens-mediated gene transfer system |
title_full_unstemmed | P351 Genetic recombination between the main human skin commensal fungus, Malassezia restricta, and M. globosa using an Agrobacterium tumefaciens-mediated gene transfer system |
title_short | P351 Genetic recombination between the main human skin commensal fungus, Malassezia restricta, and M. globosa using an Agrobacterium tumefaciens-mediated gene transfer system |
title_sort | p351 genetic recombination between the main human skin commensal fungus, malassezia restricta, and m. globosa using an agrobacterium tumefaciens-mediated gene transfer system |
topic | Oral Presentations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9509928/ http://dx.doi.org/10.1093/mmy/myac072.P351 |
work_keys_str_mv | AT chootomi p351geneticrecombinationbetweenthemainhumanskincommensalfungusmalasseziarestrictaandmglobosausinganagrobacteriumtumefaciensmediatedgenetransfersystem AT matsumotoyasuhiko p351geneticrecombinationbetweenthemainhumanskincommensalfungusmalasseziarestrictaandmglobosausinganagrobacteriumtumefaciensmediatedgenetransfersystem AT yamadatsuyoshi p351geneticrecombinationbetweenthemainhumanskincommensalfungusmalasseziarestrictaandmglobosausinganagrobacteriumtumefaciensmediatedgenetransfersystem AT sugitatakashi p351geneticrecombinationbetweenthemainhumanskincommensalfungusmalasseziarestrictaandmglobosausinganagrobacteriumtumefaciensmediatedgenetransfersystem |