(1)H, (13)C, (15)N resonance assignment of the enzyme KdgF from Bacteroides eggerthii

To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In thi...

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Detalles Bibliográficos
Autores principales: Petersen, Agnes Beenfeldt, Christensen, Idd Andrea, Rønne, Mette E., Stender, Emil G. P., Teze, David, Svensson, Birte, Aachmann, Finn Lillelund
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9510102/
https://www.ncbi.nlm.nih.gov/pubmed/36042150
http://dx.doi.org/10.1007/s12104-022-10102-6
Descripción
Sumario:To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the (1)H, (13)C, and (15)N NMR chemical shift assignment for BeKdgF is reported.

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