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Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method
Enterococcus faecalis is a serious problem for hospitals and can spread from patient to patient. Most of the current detection methods are associated with limitations associated with the need for trained personnel; they are also time-consuming. Thus, it is necessary to develop rapid and accurate det...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9510690/ https://www.ncbi.nlm.nih.gov/pubmed/36171761 http://dx.doi.org/10.3389/fcimb.2022.991849 |
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author | Zhu, Bo Hu, Juan Li, Xuelian Li, Xiaomin Wang, Lei Fan, Shihui Jin, Xin Wang, Kun Zhao, Weiguo Zhu, Wenjun Chen, Cheng Wang, Zilu Lu, Yingzhi |
author_facet | Zhu, Bo Hu, Juan Li, Xuelian Li, Xiaomin Wang, Lei Fan, Shihui Jin, Xin Wang, Kun Zhao, Weiguo Zhu, Wenjun Chen, Cheng Wang, Zilu Lu, Yingzhi |
author_sort | Zhu, Bo |
collection | PubMed |
description | Enterococcus faecalis is a serious problem for hospitals and can spread from patient to patient. Most of the current detection methods are associated with limitations associated with the need for trained personnel; they are also time-consuming. Thus, it is necessary to develop rapid and accurate detection methods to control the spread of E. faecalis. In this study, we developed a rapid and accurate detection method for E. faecalis using recombinase polymerase amplification (RPA) combined with a lateral flow strip (LFS). This method could be completed in approximately 35 min at 37°C. The limit of detection was 10 CFU/µL, irrespective of whether the templates were pure or complex. This method also showed good specificity and compatibility. In total, 278 clinical samples were tested using the RPA-LFS method; the detection accuracy was equal to that of the conventional qPCR method. This visualized isothermal amplification method could be useful for the future on-site detection of E. faecalis. |
format | Online Article Text |
id | pubmed-9510690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95106902022-09-27 Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method Zhu, Bo Hu, Juan Li, Xuelian Li, Xiaomin Wang, Lei Fan, Shihui Jin, Xin Wang, Kun Zhao, Weiguo Zhu, Wenjun Chen, Cheng Wang, Zilu Lu, Yingzhi Front Cell Infect Microbiol Cellular and Infection Microbiology Enterococcus faecalis is a serious problem for hospitals and can spread from patient to patient. Most of the current detection methods are associated with limitations associated with the need for trained personnel; they are also time-consuming. Thus, it is necessary to develop rapid and accurate detection methods to control the spread of E. faecalis. In this study, we developed a rapid and accurate detection method for E. faecalis using recombinase polymerase amplification (RPA) combined with a lateral flow strip (LFS). This method could be completed in approximately 35 min at 37°C. The limit of detection was 10 CFU/µL, irrespective of whether the templates were pure or complex. This method also showed good specificity and compatibility. In total, 278 clinical samples were tested using the RPA-LFS method; the detection accuracy was equal to that of the conventional qPCR method. This visualized isothermal amplification method could be useful for the future on-site detection of E. faecalis. Frontiers Media S.A. 2022-09-12 /pmc/articles/PMC9510690/ /pubmed/36171761 http://dx.doi.org/10.3389/fcimb.2022.991849 Text en Copyright © 2022 Zhu, Hu, Li, Li, Wang, Fan, Jin, Wang, Zhao, Zhu, Chen, Wang and Lu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Zhu, Bo Hu, Juan Li, Xuelian Li, Xiaomin Wang, Lei Fan, Shihui Jin, Xin Wang, Kun Zhao, Weiguo Zhu, Wenjun Chen, Cheng Wang, Zilu Lu, Yingzhi Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method |
title | Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method |
title_full | Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method |
title_fullStr | Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method |
title_full_unstemmed | Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method |
title_short | Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method |
title_sort | rapid and specific detection of enterococcus faecalis with a visualized isothermal amplification method |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9510690/ https://www.ncbi.nlm.nih.gov/pubmed/36171761 http://dx.doi.org/10.3389/fcimb.2022.991849 |
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