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Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics
The realization of electrochemical nucleic acid amplification tests (NAATs) at the point of care (POC) is highly desirable, but it remains a challenge given their high cost and lack of true portability/miniaturization. Here we show that mass-produced, industrial standardized, printed circuit boards...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9511072/ https://www.ncbi.nlm.nih.gov/pubmed/35993403 http://dx.doi.org/10.1039/d2an00923d |
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author | Toldrà, Anna Ainla, Alar Khaliliazar, Shirin Landin, Roman Chondrogiannis, Georgios Hanze, Martin Réu, Pedro Hamedi, Mahiar M. |
author_facet | Toldrà, Anna Ainla, Alar Khaliliazar, Shirin Landin, Roman Chondrogiannis, Georgios Hanze, Martin Réu, Pedro Hamedi, Mahiar M. |
author_sort | Toldrà, Anna |
collection | PubMed |
description | The realization of electrochemical nucleic acid amplification tests (NAATs) at the point of care (POC) is highly desirable, but it remains a challenge given their high cost and lack of true portability/miniaturization. Here we show that mass-produced, industrial standardized, printed circuit boards (PCBs) can be repurposed to act as near-zero cost electrodes for self-assembled monolayer-based DNA biosensing, and further integration with a custom-designed and low-cost portable potentiostat. To show the analytical capability of this system, we developed a NAAT using isothermal recombinase polymerase amplification, bypassing the need of thermal cyclers, followed by an electrochemical readout relying on a sandwich hybridization assay. We used our sensor and device for analytical detection of the toxic microalgae Ostreopsis cf. ovata as a proof of concept. This work shows the potential of PCBs and open-source electronics to be used as powerful POC DNA biosensors at a low-cost. |
format | Online Article Text |
id | pubmed-9511072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-95110722022-10-31 Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics Toldrà, Anna Ainla, Alar Khaliliazar, Shirin Landin, Roman Chondrogiannis, Georgios Hanze, Martin Réu, Pedro Hamedi, Mahiar M. Analyst Chemistry The realization of electrochemical nucleic acid amplification tests (NAATs) at the point of care (POC) is highly desirable, but it remains a challenge given their high cost and lack of true portability/miniaturization. Here we show that mass-produced, industrial standardized, printed circuit boards (PCBs) can be repurposed to act as near-zero cost electrodes for self-assembled monolayer-based DNA biosensing, and further integration with a custom-designed and low-cost portable potentiostat. To show the analytical capability of this system, we developed a NAAT using isothermal recombinase polymerase amplification, bypassing the need of thermal cyclers, followed by an electrochemical readout relying on a sandwich hybridization assay. We used our sensor and device for analytical detection of the toxic microalgae Ostreopsis cf. ovata as a proof of concept. This work shows the potential of PCBs and open-source electronics to be used as powerful POC DNA biosensors at a low-cost. The Royal Society of Chemistry 2022-08-15 /pmc/articles/PMC9511072/ /pubmed/35993403 http://dx.doi.org/10.1039/d2an00923d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Toldrà, Anna Ainla, Alar Khaliliazar, Shirin Landin, Roman Chondrogiannis, Georgios Hanze, Martin Réu, Pedro Hamedi, Mahiar M. Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics |
title | Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics |
title_full | Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics |
title_fullStr | Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics |
title_full_unstemmed | Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics |
title_short | Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics |
title_sort | portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9511072/ https://www.ncbi.nlm.nih.gov/pubmed/35993403 http://dx.doi.org/10.1039/d2an00923d |
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