Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression

OBJECTIVE: Our previous work found COX4I2 was associated with angiogenesis in pheochromocytoma. The purpose of this study was to explore the role of COX4I2 in regulating angiogenesis in pheochromocytoma. METHODS: Distribution of COX4I2 was evaluated by scRNA-seq in one case of pheochromocytoma and t...

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Autores principales: Mao, Yongxin, Zhuo, Ran, Ma, Wenming, Dai, Jun, Alimu, Parehe, Fang, Chen, Xu, Danfeng, Ye, Lei, Wang, Weiqing, Sun, Fukang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Oncology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9511905/
https://www.ncbi.nlm.nih.gov/pubmed/36172142
http://dx.doi.org/10.3389/fonc.2022.938123
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author Mao, Yongxin
Zhuo, Ran
Ma, Wenming
Dai, Jun
Alimu, Parehe
Fang, Chen
Xu, Danfeng
Ye, Lei
Wang, Weiqing
Sun, Fukang
author_facet Mao, Yongxin
Zhuo, Ran
Ma, Wenming
Dai, Jun
Alimu, Parehe
Fang, Chen
Xu, Danfeng
Ye, Lei
Wang, Weiqing
Sun, Fukang
author_sort Mao, Yongxin
collection PubMed
description OBJECTIVE: Our previous work found COX4I2 was associated with angiogenesis in pheochromocytoma. The purpose of this study was to explore the role of COX4I2 in regulating angiogenesis in pheochromocytoma. METHODS: Distribution of COX4I2 was evaluated by scRNA-seq in one case of pheochromocytoma and the findings were verified by immunostaining. COX4I2 was further knocked down in target cells. Changes of angiogenesis-related genes were evaluated by qPCR in target cells. RESULTS: The scRNA-seq revealed high mRNA expression of COX4I2 in fibroblasts rather than tumor cells. Immunostaining of COX4I2 confirmed its distribution in fibroblasts. Knocking down COX4I2 in NIH3T3 cell line led to significant reduction of angiogenesis-related genes, especially ANG1 and HGF. CONCLUSIONS: Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression, possibly by affecting ANG1 and HGF.
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spelling pubmed-95119052022-09-27 Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression Mao, Yongxin Zhuo, Ran Ma, Wenming Dai, Jun Alimu, Parehe Fang, Chen Xu, Danfeng Ye, Lei Wang, Weiqing Sun, Fukang Front Oncol Oncology OBJECTIVE: Our previous work found COX4I2 was associated with angiogenesis in pheochromocytoma. The purpose of this study was to explore the role of COX4I2 in regulating angiogenesis in pheochromocytoma. METHODS: Distribution of COX4I2 was evaluated by scRNA-seq in one case of pheochromocytoma and the findings were verified by immunostaining. COX4I2 was further knocked down in target cells. Changes of angiogenesis-related genes were evaluated by qPCR in target cells. RESULTS: The scRNA-seq revealed high mRNA expression of COX4I2 in fibroblasts rather than tumor cells. Immunostaining of COX4I2 confirmed its distribution in fibroblasts. Knocking down COX4I2 in NIH3T3 cell line led to significant reduction of angiogenesis-related genes, especially ANG1 and HGF. CONCLUSIONS: Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression, possibly by affecting ANG1 and HGF. Frontiers Media S.A. 2022-09-12 /pmc/articles/PMC9511905/ /pubmed/36172142 http://dx.doi.org/10.3389/fonc.2022.938123 Text en Copyright © 2022 Mao, Zhuo, Ma, Dai, Alimu, Fang, Xu, Ye, Wang and Sun https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Mao, Yongxin
Zhuo, Ran
Ma, Wenming
Dai, Jun
Alimu, Parehe
Fang, Chen
Xu, Danfeng
Ye, Lei
Wang, Weiqing
Sun, Fukang
Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression
title Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression
title_full Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression
title_fullStr Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression
title_full_unstemmed Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression
title_short Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression
title_sort fibroblasts mediate the angiogenesis of pheochromocytoma by increasing cox4i2 expression
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9511905/
https://www.ncbi.nlm.nih.gov/pubmed/36172142
http://dx.doi.org/10.3389/fonc.2022.938123
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