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Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain

BACKGROUND: Recent studies have demonstrated the contribution of non-coding RNAs (ncRNAs) to neuropathic pain. However, the expression profile of ncRNAs in the trigeminal ganglion (TG) and their functional mechanism underlying trigeminal neuropathic pain are still unclear. METHODS: In the present st...

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Autores principales: Fang, Zhong-Han, Liao, Hong-Lin, Tang, Qing-Feng, Liu, Ya-Jing, Zhang, Yan-Yan, Lin, Jiu, Yu, Hao-Peng, Zhou, Cheng, Li, Chun-Jie, Liu, Fei, Shen, Jie-Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512292/
https://www.ncbi.nlm.nih.gov/pubmed/36171980
http://dx.doi.org/10.2147/JPR.S382692
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author Fang, Zhong-Han
Liao, Hong-Lin
Tang, Qing-Feng
Liu, Ya-Jing
Zhang, Yan-Yan
Lin, Jiu
Yu, Hao-Peng
Zhou, Cheng
Li, Chun-Jie
Liu, Fei
Shen, Jie-Fei
author_facet Fang, Zhong-Han
Liao, Hong-Lin
Tang, Qing-Feng
Liu, Ya-Jing
Zhang, Yan-Yan
Lin, Jiu
Yu, Hao-Peng
Zhou, Cheng
Li, Chun-Jie
Liu, Fei
Shen, Jie-Fei
author_sort Fang, Zhong-Han
collection PubMed
description BACKGROUND: Recent studies have demonstrated the contribution of non-coding RNAs (ncRNAs) to neuropathic pain. However, the expression profile of ncRNAs in the trigeminal ganglion (TG) and their functional mechanism underlying trigeminal neuropathic pain are still unclear. METHODS: In the present study, the trigeminal neuropathic pain model induced by chronic constriction injury of the infraorbital nerve (CCI-ION) was used to study the expression profile and potential regulatory mechanism of miRNAs, lncRNAs, circRNAs, and mRNAs in the TG by RNA-sequencing (RNA-seq) and bioinformatics analysis. CCI-ION mice suffered from mechanical allodynia from 3 days to 28 days after surgery. RESULTS: The RNA-seq results discovered 67 miRNAs, 216 lncRNAs, 14 circRNAs, 595 mRNAs, and 421 genes were differentially expressed (DE) in the TG of CCI-ION mice 7 days after surgery. And 39 DEGs were known pain genes. Besides, 5 and 35 pain-related DE mRNAs could be targeted by 6 DE miRNAs and 107 DE lncRNAs, respectively. And 23 pain-related DEGs had protein–protein interactions (PPI) with each other. GO analysis indicated membrane-related cell components and binding-related molecular functions were significantly enriched. KEGG analysis showed that nociception-related signaling pathways were significantly enriched for DE ncRNAs and DEGs. Finally, the competing endogenous RNA (ceRNA) regulatory network of DE lncRNA/DE circRNA-DE miRNA-DE mRNA existed in the TG of mice with trigeminal neuropathic pain. CONCLUSION: Our findings demonstrate ncRNAs are involved in the development of trigeminal neuropathic pain, possibly through the ceRNA mechanism, which brings a new bright into the study of trigeminal neuropathic pain and the development of novel treatments targeting ncRNAs.
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spelling pubmed-95122922022-09-27 Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain Fang, Zhong-Han Liao, Hong-Lin Tang, Qing-Feng Liu, Ya-Jing Zhang, Yan-Yan Lin, Jiu Yu, Hao-Peng Zhou, Cheng Li, Chun-Jie Liu, Fei Shen, Jie-Fei J Pain Res Original Research BACKGROUND: Recent studies have demonstrated the contribution of non-coding RNAs (ncRNAs) to neuropathic pain. However, the expression profile of ncRNAs in the trigeminal ganglion (TG) and their functional mechanism underlying trigeminal neuropathic pain are still unclear. METHODS: In the present study, the trigeminal neuropathic pain model induced by chronic constriction injury of the infraorbital nerve (CCI-ION) was used to study the expression profile and potential regulatory mechanism of miRNAs, lncRNAs, circRNAs, and mRNAs in the TG by RNA-sequencing (RNA-seq) and bioinformatics analysis. CCI-ION mice suffered from mechanical allodynia from 3 days to 28 days after surgery. RESULTS: The RNA-seq results discovered 67 miRNAs, 216 lncRNAs, 14 circRNAs, 595 mRNAs, and 421 genes were differentially expressed (DE) in the TG of CCI-ION mice 7 days after surgery. And 39 DEGs were known pain genes. Besides, 5 and 35 pain-related DE mRNAs could be targeted by 6 DE miRNAs and 107 DE lncRNAs, respectively. And 23 pain-related DEGs had protein–protein interactions (PPI) with each other. GO analysis indicated membrane-related cell components and binding-related molecular functions were significantly enriched. KEGG analysis showed that nociception-related signaling pathways were significantly enriched for DE ncRNAs and DEGs. Finally, the competing endogenous RNA (ceRNA) regulatory network of DE lncRNA/DE circRNA-DE miRNA-DE mRNA existed in the TG of mice with trigeminal neuropathic pain. CONCLUSION: Our findings demonstrate ncRNAs are involved in the development of trigeminal neuropathic pain, possibly through the ceRNA mechanism, which brings a new bright into the study of trigeminal neuropathic pain and the development of novel treatments targeting ncRNAs. Dove 2022-09-22 /pmc/articles/PMC9512292/ /pubmed/36171980 http://dx.doi.org/10.2147/JPR.S382692 Text en © 2022 Fang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Fang, Zhong-Han
Liao, Hong-Lin
Tang, Qing-Feng
Liu, Ya-Jing
Zhang, Yan-Yan
Lin, Jiu
Yu, Hao-Peng
Zhou, Cheng
Li, Chun-Jie
Liu, Fei
Shen, Jie-Fei
Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain
title Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain
title_full Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain
title_fullStr Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain
title_full_unstemmed Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain
title_short Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain
title_sort interactions among non-coding rnas and mrnas in the trigeminal ganglion associated with neuropathic pain
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512292/
https://www.ncbi.nlm.nih.gov/pubmed/36171980
http://dx.doi.org/10.2147/JPR.S382692
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