Multiscale fluorescence imaging of living samples

Fluorescence microscopy is a highly effective tool for interrogating biological structure and function, particularly when imaging across multiple spatiotemporal scales. Here we survey recent innovations and applications in the relatively understudied area of multiscale fluorescence imaging of living...

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Detalles Bibliográficos
Autores principales: Wu, Yicong, Shroff, Hari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512758/
https://www.ncbi.nlm.nih.gov/pubmed/36036808
http://dx.doi.org/10.1007/s00418-022-02147-4
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author Wu, Yicong
Shroff, Hari
author_facet Wu, Yicong
Shroff, Hari
author_sort Wu, Yicong
collection PubMed
description Fluorescence microscopy is a highly effective tool for interrogating biological structure and function, particularly when imaging across multiple spatiotemporal scales. Here we survey recent innovations and applications in the relatively understudied area of multiscale fluorescence imaging of living samples. We discuss fundamental challenges in live multiscale imaging and describe successful examples that highlight the power of this approach. We attempt to synthesize general strategies from these test cases, aiming to help accelerate progress in this exciting area.
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spelling pubmed-95127582022-09-28 Multiscale fluorescence imaging of living samples Wu, Yicong Shroff, Hari Histochem Cell Biol Review Fluorescence microscopy is a highly effective tool for interrogating biological structure and function, particularly when imaging across multiple spatiotemporal scales. Here we survey recent innovations and applications in the relatively understudied area of multiscale fluorescence imaging of living samples. We discuss fundamental challenges in live multiscale imaging and describe successful examples that highlight the power of this approach. We attempt to synthesize general strategies from these test cases, aiming to help accelerate progress in this exciting area. Springer Berlin Heidelberg 2022-08-29 2022 /pmc/articles/PMC9512758/ /pubmed/36036808 http://dx.doi.org/10.1007/s00418-022-02147-4 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Wu, Yicong
Shroff, Hari
Multiscale fluorescence imaging of living samples
title Multiscale fluorescence imaging of living samples
title_full Multiscale fluorescence imaging of living samples
title_fullStr Multiscale fluorescence imaging of living samples
title_full_unstemmed Multiscale fluorescence imaging of living samples
title_short Multiscale fluorescence imaging of living samples
title_sort multiscale fluorescence imaging of living samples
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512758/
https://www.ncbi.nlm.nih.gov/pubmed/36036808
http://dx.doi.org/10.1007/s00418-022-02147-4
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