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Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome

Prebiotic fibers, polyphenols and other molecular components of food crops significantly affect the composition and function of the human gut microbiome and human health. The abundance of these, frequently uncharacterized, microbiome-active components vary within individual crop species. Here, we em...

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Autores principales: Yang, Qinnan, Van Haute, Mallory, Korth, Nate, Sattler, Scott E., Toy, John, Rose, Devin J., Schnable, James C., Benson, Andrew K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513080/
https://www.ncbi.nlm.nih.gov/pubmed/36163368
http://dx.doi.org/10.1038/s41467-022-33419-1
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author Yang, Qinnan
Van Haute, Mallory
Korth, Nate
Sattler, Scott E.
Toy, John
Rose, Devin J.
Schnable, James C.
Benson, Andrew K.
author_facet Yang, Qinnan
Van Haute, Mallory
Korth, Nate
Sattler, Scott E.
Toy, John
Rose, Devin J.
Schnable, James C.
Benson, Andrew K.
author_sort Yang, Qinnan
collection PubMed
description Prebiotic fibers, polyphenols and other molecular components of food crops significantly affect the composition and function of the human gut microbiome and human health. The abundance of these, frequently uncharacterized, microbiome-active components vary within individual crop species. Here, we employ high throughput in vitro fermentations of pre-digested grain using a human microbiome to identify segregating genetic loci in a food crop, sorghum, that alter the composition and function of human gut microbes. Evaluating grain produced by 294 sorghum recombinant inbreds identifies 10 loci in the sorghum genome associated with variation in the abundance of microbial taxa and/or microbial metabolites. Two loci co-localize with sorghum genes regulating the biosynthesis of condensed tannins. We validate that condensed tannins stimulate the growth of microbes associated with these two loci. Our work illustrates the potential for genetic analysis to systematically discover and characterize molecular components of food crops that influence the human gut microbiome.
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spelling pubmed-95130802022-09-28 Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome Yang, Qinnan Van Haute, Mallory Korth, Nate Sattler, Scott E. Toy, John Rose, Devin J. Schnable, James C. Benson, Andrew K. Nat Commun Article Prebiotic fibers, polyphenols and other molecular components of food crops significantly affect the composition and function of the human gut microbiome and human health. The abundance of these, frequently uncharacterized, microbiome-active components vary within individual crop species. Here, we employ high throughput in vitro fermentations of pre-digested grain using a human microbiome to identify segregating genetic loci in a food crop, sorghum, that alter the composition and function of human gut microbes. Evaluating grain produced by 294 sorghum recombinant inbreds identifies 10 loci in the sorghum genome associated with variation in the abundance of microbial taxa and/or microbial metabolites. Two loci co-localize with sorghum genes regulating the biosynthesis of condensed tannins. We validate that condensed tannins stimulate the growth of microbes associated with these two loci. Our work illustrates the potential for genetic analysis to systematically discover and characterize molecular components of food crops that influence the human gut microbiome. Nature Publishing Group UK 2022-09-26 /pmc/articles/PMC9513080/ /pubmed/36163368 http://dx.doi.org/10.1038/s41467-022-33419-1 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yang, Qinnan
Van Haute, Mallory
Korth, Nate
Sattler, Scott E.
Toy, John
Rose, Devin J.
Schnable, James C.
Benson, Andrew K.
Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome
title Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome
title_full Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome
title_fullStr Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome
title_full_unstemmed Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome
title_short Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome
title_sort genetic analysis of seed traits in sorghum bicolor that affect the human gut microbiome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513080/
https://www.ncbi.nlm.nih.gov/pubmed/36163368
http://dx.doi.org/10.1038/s41467-022-33419-1
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