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Characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia
BACKGROUND: Pneumonia is a leading cause of non-relapse mortality after hematopoietic stem cell transplantation (HSCT), and the lower respiratory tract (LRT) microbiome has been proven to be associated with various respiratory diseases. However, little is known about the characteristics of the LRT m...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513191/ https://www.ncbi.nlm.nih.gov/pubmed/36176576 http://dx.doi.org/10.3389/fcimb.2022.943317 |
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author | He, Yukun Li, Jia Yu, Wenyi Zheng, Yali Yang, Donghong Xu, Yu Zhao, Lili Ma, Xinqian Gong, Pihua Gao, Zhancheng |
author_facet | He, Yukun Li, Jia Yu, Wenyi Zheng, Yali Yang, Donghong Xu, Yu Zhao, Lili Ma, Xinqian Gong, Pihua Gao, Zhancheng |
author_sort | He, Yukun |
collection | PubMed |
description | BACKGROUND: Pneumonia is a leading cause of non-relapse mortality after hematopoietic stem cell transplantation (HSCT), and the lower respiratory tract (LRT) microbiome has been proven to be associated with various respiratory diseases. However, little is known about the characteristics of the LRT microbiome in patients with post-HSCT compared to healthy controls (HC) and community-acquired pneumonia (CAP). METHODS: Bronchoalveolar lavage samples from 55 patients with post-HSCT pneumonia, 44 patients with CAP, and 30 healthy volunteers were used to detect microbiota using 16S rRNA gene sequencing. RESULTS: The diversity of the LRT microbiome significantly decreased in patients with post-HSCT pneumonia, and the overall community was different from the CAP and HC groups. At the phylum level, post-HSCT pneumonia samples had a high abundance of Actinobacteria and a relatively low abundance of Bacteroidetes. The same is true for non-survivors compared with survivors in patients with post-HSCT pneumonia. At the genus level, the abundances of Pseudomonas, Acinetobacter, Burkholderia, and Mycobacterium were prominent in the pneumonia group after HSCT. On the other hand, gut-associated bacteria, Enterococcus were more abundant in the non-survivors. Some pathways concerning amino acid and lipid metabolism were predicted to be altered in patients with post-HSCT pneumonia. CONCLUSIONS: Our results reveal that the LRT microbiome in patients with post-HSCT pneumonia differs from CAP patients and healthy controls, which could be associated with the outcome. The LRT microbiota could be a target for intervention during post-HSCT pneumonia. |
format | Online Article Text |
id | pubmed-9513191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95131912022-09-28 Characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia He, Yukun Li, Jia Yu, Wenyi Zheng, Yali Yang, Donghong Xu, Yu Zhao, Lili Ma, Xinqian Gong, Pihua Gao, Zhancheng Front Cell Infect Microbiol Cellular and Infection Microbiology BACKGROUND: Pneumonia is a leading cause of non-relapse mortality after hematopoietic stem cell transplantation (HSCT), and the lower respiratory tract (LRT) microbiome has been proven to be associated with various respiratory diseases. However, little is known about the characteristics of the LRT microbiome in patients with post-HSCT compared to healthy controls (HC) and community-acquired pneumonia (CAP). METHODS: Bronchoalveolar lavage samples from 55 patients with post-HSCT pneumonia, 44 patients with CAP, and 30 healthy volunteers were used to detect microbiota using 16S rRNA gene sequencing. RESULTS: The diversity of the LRT microbiome significantly decreased in patients with post-HSCT pneumonia, and the overall community was different from the CAP and HC groups. At the phylum level, post-HSCT pneumonia samples had a high abundance of Actinobacteria and a relatively low abundance of Bacteroidetes. The same is true for non-survivors compared with survivors in patients with post-HSCT pneumonia. At the genus level, the abundances of Pseudomonas, Acinetobacter, Burkholderia, and Mycobacterium were prominent in the pneumonia group after HSCT. On the other hand, gut-associated bacteria, Enterococcus were more abundant in the non-survivors. Some pathways concerning amino acid and lipid metabolism were predicted to be altered in patients with post-HSCT pneumonia. CONCLUSIONS: Our results reveal that the LRT microbiome in patients with post-HSCT pneumonia differs from CAP patients and healthy controls, which could be associated with the outcome. The LRT microbiota could be a target for intervention during post-HSCT pneumonia. Frontiers Media S.A. 2022-09-13 /pmc/articles/PMC9513191/ /pubmed/36176576 http://dx.doi.org/10.3389/fcimb.2022.943317 Text en Copyright © 2022 He, Li, Yu, Zheng, Yang, Xu, Zhao, Ma, Gong and Gao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology He, Yukun Li, Jia Yu, Wenyi Zheng, Yali Yang, Donghong Xu, Yu Zhao, Lili Ma, Xinqian Gong, Pihua Gao, Zhancheng Characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia |
title | Characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia |
title_full | Characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia |
title_fullStr | Characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia |
title_full_unstemmed | Characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia |
title_short | Characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia |
title_sort | characteristics of lower respiratory tract microbiota in the patients with post-hematopoietic stem cell transplantation pneumonia |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513191/ https://www.ncbi.nlm.nih.gov/pubmed/36176576 http://dx.doi.org/10.3389/fcimb.2022.943317 |
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