Simultaneous analysis of social behaviors and neural responses in mice using round social arena system

Stable and accurate capturing of detailed social behaviors is essential for studying rodent sociability. Here, we introduce a round social arena (RSA) system that enables close-up monitoring of detailed social behaviors in mice. We describe the steps to build RSA apparatus and set up the wiring for...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Sunwhi, Kim, Yong-Eun, Kim, Il Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513260/
https://www.ncbi.nlm.nih.gov/pubmed/36153733
http://dx.doi.org/10.1016/j.xpro.2022.101722
Descripción
Sumario:Stable and accurate capturing of detailed social behaviors is essential for studying rodent sociability. Here, we introduce a round social arena (RSA) system that enables close-up monitoring of detailed social behaviors in mice. We describe the steps to build RSA apparatus and set up the wiring for video synchronization. We then detail how to conduct RSA experiment with simultaneous Ca(2+) imaging or optogenetics. This protocol also includes a custom MATLAB script for aligning the behavioral dataset to Ca(2+) trace data. For complete details on the use and execution of this protocol, please refer to Kim et al. (2022).

Ejemplares similares