Simultaneous analysis of social behaviors and neural responses in mice using round social arena system

Stable and accurate capturing of detailed social behaviors is essential for studying rodent sociability. Here, we introduce a round social arena (RSA) system that enables close-up monitoring of detailed social behaviors in mice. We describe the steps to build RSA apparatus and set up the wiring for...

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Detalles Bibliográficos
Autores principales: Kim, Sunwhi, Kim, Yong-Eun, Kim, Il Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513260/
https://www.ncbi.nlm.nih.gov/pubmed/36153733
http://dx.doi.org/10.1016/j.xpro.2022.101722
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author Kim, Sunwhi
Kim, Yong-Eun
Kim, Il Hwan
author_facet Kim, Sunwhi
Kim, Yong-Eun
Kim, Il Hwan
author_sort Kim, Sunwhi
collection PubMed
description Stable and accurate capturing of detailed social behaviors is essential for studying rodent sociability. Here, we introduce a round social arena (RSA) system that enables close-up monitoring of detailed social behaviors in mice. We describe the steps to build RSA apparatus and set up the wiring for video synchronization. We then detail how to conduct RSA experiment with simultaneous Ca(2+) imaging or optogenetics. This protocol also includes a custom MATLAB script for aligning the behavioral dataset to Ca(2+) trace data. For complete details on the use and execution of this protocol, please refer to Kim et al. (2022).
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spelling pubmed-95132602022-09-28 Simultaneous analysis of social behaviors and neural responses in mice using round social arena system Kim, Sunwhi Kim, Yong-Eun Kim, Il Hwan STAR Protoc Protocol Stable and accurate capturing of detailed social behaviors is essential for studying rodent sociability. Here, we introduce a round social arena (RSA) system that enables close-up monitoring of detailed social behaviors in mice. We describe the steps to build RSA apparatus and set up the wiring for video synchronization. We then detail how to conduct RSA experiment with simultaneous Ca(2+) imaging or optogenetics. This protocol also includes a custom MATLAB script for aligning the behavioral dataset to Ca(2+) trace data. For complete details on the use and execution of this protocol, please refer to Kim et al. (2022). Elsevier 2022-09-24 /pmc/articles/PMC9513260/ /pubmed/36153733 http://dx.doi.org/10.1016/j.xpro.2022.101722 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kim, Sunwhi
Kim, Yong-Eun
Kim, Il Hwan
Simultaneous analysis of social behaviors and neural responses in mice using round social arena system
title Simultaneous analysis of social behaviors and neural responses in mice using round social arena system
title_full Simultaneous analysis of social behaviors and neural responses in mice using round social arena system
title_fullStr Simultaneous analysis of social behaviors and neural responses in mice using round social arena system
title_full_unstemmed Simultaneous analysis of social behaviors and neural responses in mice using round social arena system
title_short Simultaneous analysis of social behaviors and neural responses in mice using round social arena system
title_sort simultaneous analysis of social behaviors and neural responses in mice using round social arena system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513260/
https://www.ncbi.nlm.nih.gov/pubmed/36153733
http://dx.doi.org/10.1016/j.xpro.2022.101722
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