Cargando…

Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility

Embryo mortality rate, which can reach up to 40% in avian species, is a major issue for breeding. It is therefore important to identify new embryo development biomarkers for genetic selection to improve reproductive performances. We have recently shown that chemerin is expressed in the oviductal hen...

Descripción completa

Detalles Bibliográficos
Autores principales: Bernardi, Ophélie, Reverchon, Maxime, Estienne, Anthony, Baumard, Yannick, Ramé, Christelle, Brossaud, Adeline, Combarnous, Yves, Froment, Pascal, Dupont, Joëlle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513417/
https://www.ncbi.nlm.nih.gov/pubmed/36176771
http://dx.doi.org/10.3389/fphys.2022.1012212
_version_ 1784798058183131136
author Bernardi, Ophélie
Reverchon, Maxime
Estienne, Anthony
Baumard, Yannick
Ramé, Christelle
Brossaud, Adeline
Combarnous, Yves
Froment, Pascal
Dupont, Joëlle
author_facet Bernardi, Ophélie
Reverchon, Maxime
Estienne, Anthony
Baumard, Yannick
Ramé, Christelle
Brossaud, Adeline
Combarnous, Yves
Froment, Pascal
Dupont, Joëlle
author_sort Bernardi, Ophélie
collection PubMed
description Embryo mortality rate, which can reach up to 40% in avian species, is a major issue for breeding. It is therefore important to identify new embryo development biomarkers for genetic selection to improve reproductive performances. We have recently shown that chemerin is expressed in the oviductal hen magnum, accumulates in egg white, is correlated with embryo survival and could thus be used as a molecular marker of embryo development. Eggs from seven hen breeds (n = 70) were collected during five successive days at the end of the laying period. After weighing eggs, yolk and albumen, an egg white sample from each egg was collected and a blood sample was taken from each hen. Chemerin concentrations in albumen and blood samples were measured by a specific home made ELISA assay. Hen’s plasma and egg’s albumen chemerin levels were found to be correlated with reproductive parameters such as fecundity, fertility, embryo mortality, hatchability and laying rates. The inter-hen chemerin level variability in albumen was higher than intra-hen except for one breed (R+). We observed significantly different levels of chemerin in egg white between breeds. However, chemerin concentrations in egg white were not significantly associated to variations of hen plasma chemerin levels. Interestingly, we observed negative correlations between albumen chemerin concentrations and egg weight (r = −0.43, p = 0.001), between albumen weight (r = −0.40, p = 0.002), and between yolk weight (r = −0.28, p = 0.03). We also showed negative correlations between egg white chemerin concentrations and fecundity (r = −0.32, p = 0.011) and fertility (r = −0.27, p = 0.04) whereas no significant correlation was observed with the laying rate. Taken together, these results suggest that egg white chemerin concentration might be a good biomarker for genetic selection for egg weight and fertility in hens, provided these data are confirmed on a larger scale.
format Online
Article
Text
id pubmed-9513417
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95134172022-09-28 Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility Bernardi, Ophélie Reverchon, Maxime Estienne, Anthony Baumard, Yannick Ramé, Christelle Brossaud, Adeline Combarnous, Yves Froment, Pascal Dupont, Joëlle Front Physiol Physiology Embryo mortality rate, which can reach up to 40% in avian species, is a major issue for breeding. It is therefore important to identify new embryo development biomarkers for genetic selection to improve reproductive performances. We have recently shown that chemerin is expressed in the oviductal hen magnum, accumulates in egg white, is correlated with embryo survival and could thus be used as a molecular marker of embryo development. Eggs from seven hen breeds (n = 70) were collected during five successive days at the end of the laying period. After weighing eggs, yolk and albumen, an egg white sample from each egg was collected and a blood sample was taken from each hen. Chemerin concentrations in albumen and blood samples were measured by a specific home made ELISA assay. Hen’s plasma and egg’s albumen chemerin levels were found to be correlated with reproductive parameters such as fecundity, fertility, embryo mortality, hatchability and laying rates. The inter-hen chemerin level variability in albumen was higher than intra-hen except for one breed (R+). We observed significantly different levels of chemerin in egg white between breeds. However, chemerin concentrations in egg white were not significantly associated to variations of hen plasma chemerin levels. Interestingly, we observed negative correlations between albumen chemerin concentrations and egg weight (r = −0.43, p = 0.001), between albumen weight (r = −0.40, p = 0.002), and between yolk weight (r = −0.28, p = 0.03). We also showed negative correlations between egg white chemerin concentrations and fecundity (r = −0.32, p = 0.011) and fertility (r = −0.27, p = 0.04) whereas no significant correlation was observed with the laying rate. Taken together, these results suggest that egg white chemerin concentration might be a good biomarker for genetic selection for egg weight and fertility in hens, provided these data are confirmed on a larger scale. Frontiers Media S.A. 2022-09-13 /pmc/articles/PMC9513417/ /pubmed/36176771 http://dx.doi.org/10.3389/fphys.2022.1012212 Text en Copyright © 2022 Bernardi, Reverchon, Estienne, Baumard, Ramé, Brossaud, Combarnous, Froment and Dupont. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Bernardi, Ophélie
Reverchon, Maxime
Estienne, Anthony
Baumard, Yannick
Ramé, Christelle
Brossaud, Adeline
Combarnous, Yves
Froment, Pascal
Dupont, Joëlle
Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility
title Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility
title_full Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility
title_fullStr Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility
title_full_unstemmed Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility
title_short Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility
title_sort chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513417/
https://www.ncbi.nlm.nih.gov/pubmed/36176771
http://dx.doi.org/10.3389/fphys.2022.1012212
work_keys_str_mv AT bernardiophelie chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility
AT reverchonmaxime chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility
AT estienneanthony chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility
AT baumardyannick chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility
AT ramechristelle chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility
AT brossaudadeline chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility
AT combarnousyves chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility
AT fromentpascal chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility
AT dupontjoelle chickenwhiteeggchemerinasatoolforgeneticselectionforeggweightandhenfertility