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Effects of aflatoxin B(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks

The research evaluated the effects of Aflatoxin B1 on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks. In total, 240 7-day-old ISA chicks were randomly assigned to four treatment groups. The control group comprised chicks fed a bas...

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Autores principales: Hou, Lele, Qiu, Huiling, Li, Anping, Dong, Jihong, Zhu, Lianqin, Liu, Guowen, Chen, Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513573/
https://www.ncbi.nlm.nih.gov/pubmed/36176699
http://dx.doi.org/10.3389/fvets.2022.993039
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author Hou, Lele
Qiu, Huiling
Li, Anping
Dong, Jihong
Zhu, Lianqin
Liu, Guowen
Chen, Fu
author_facet Hou, Lele
Qiu, Huiling
Li, Anping
Dong, Jihong
Zhu, Lianqin
Liu, Guowen
Chen, Fu
author_sort Hou, Lele
collection PubMed
description The research evaluated the effects of Aflatoxin B1 on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks. In total, 240 7-day-old ISA chicks were randomly assigned to four treatment groups. The control group comprised chicks fed a basal diet. The aflatoxin (AFB(1))-treatment groups (T1, T2, and T3) comprised chicks fed the basal diet supplemented with AFB(1) at concentrations of 5, 8, and 10 μg/kg, respectively. The growth performance, antioxidant status, immune responses, and pro-inflammatory cytokine mRNA expression in all groups were measured. In the T1 treatment group (receiving the lowest AFB(1) dose), a reduction in the Newcastle disease virus antibody (NDV-Ab) titer, and increases in interleukin 2 (IL-2), IL-6, and interferon γ (IFN-γ) mRNA levels were observed on days 21 and 42 (P < 0.05). Treatment with the higher AFB(1) doses (groups T2 and T3) reduced the chicks' growth performance on days 21 and 42, measured as reductions in body weight (BW) and average daily gain (ADG) compared with the control group. In the T2 and T3 groups, the total antioxidant capacity (T-AOC), glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities, serum immunoglobulin A (IgA) and IgG levels, and IL-2, IL-6, and IFN-γ levels were also lower than in the control group. On days 21 and 42, these two groups also showed increased malondialdehyde (MDA) content, higher feed to gain ratio (F/G), and higher IL-2, IL-6, and IFN-γ mRNA levels than the control group (P < 0.05). The T2 and T3 groups also showed reduced T-AOC, NDV-Ab titer, IL-2 content, and GPx-1 mRNA levels on days 21 and 42 (P < 0.05), increased IL-6 and IFN-γ mRNA levels on day 21, and increased F/G and MDA content on day 42 (P < 0.05) compared with group (T1). Increased MDA content and IL-6 mRNA levels in the liver and ileum were observed in group T3 compared with group T2 on day 21, and lower IgM and IL-6 levels were observed on days 21 and 42 (P < 0.05). In conclusion, our data showed that AFB(1) exposure resulted in dose-dependent oxidative and inflammatory damage, immunosuppression, and a decline in the growth performance of chicks.
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spelling pubmed-95135732022-09-28 Effects of aflatoxin B(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks Hou, Lele Qiu, Huiling Li, Anping Dong, Jihong Zhu, Lianqin Liu, Guowen Chen, Fu Front Vet Sci Veterinary Science The research evaluated the effects of Aflatoxin B1 on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks. In total, 240 7-day-old ISA chicks were randomly assigned to four treatment groups. The control group comprised chicks fed a basal diet. The aflatoxin (AFB(1))-treatment groups (T1, T2, and T3) comprised chicks fed the basal diet supplemented with AFB(1) at concentrations of 5, 8, and 10 μg/kg, respectively. The growth performance, antioxidant status, immune responses, and pro-inflammatory cytokine mRNA expression in all groups were measured. In the T1 treatment group (receiving the lowest AFB(1) dose), a reduction in the Newcastle disease virus antibody (NDV-Ab) titer, and increases in interleukin 2 (IL-2), IL-6, and interferon γ (IFN-γ) mRNA levels were observed on days 21 and 42 (P < 0.05). Treatment with the higher AFB(1) doses (groups T2 and T3) reduced the chicks' growth performance on days 21 and 42, measured as reductions in body weight (BW) and average daily gain (ADG) compared with the control group. In the T2 and T3 groups, the total antioxidant capacity (T-AOC), glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities, serum immunoglobulin A (IgA) and IgG levels, and IL-2, IL-6, and IFN-γ levels were also lower than in the control group. On days 21 and 42, these two groups also showed increased malondialdehyde (MDA) content, higher feed to gain ratio (F/G), and higher IL-2, IL-6, and IFN-γ mRNA levels than the control group (P < 0.05). The T2 and T3 groups also showed reduced T-AOC, NDV-Ab titer, IL-2 content, and GPx-1 mRNA levels on days 21 and 42 (P < 0.05), increased IL-6 and IFN-γ mRNA levels on day 21, and increased F/G and MDA content on day 42 (P < 0.05) compared with group (T1). Increased MDA content and IL-6 mRNA levels in the liver and ileum were observed in group T3 compared with group T2 on day 21, and lower IgM and IL-6 levels were observed on days 21 and 42 (P < 0.05). In conclusion, our data showed that AFB(1) exposure resulted in dose-dependent oxidative and inflammatory damage, immunosuppression, and a decline in the growth performance of chicks. Frontiers Media S.A. 2022-09-13 /pmc/articles/PMC9513573/ /pubmed/36176699 http://dx.doi.org/10.3389/fvets.2022.993039 Text en Copyright © 2022 Hou, Qiu, Li, Dong, Zhu, Liu and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Hou, Lele
Qiu, Huiling
Li, Anping
Dong, Jihong
Zhu, Lianqin
Liu, Guowen
Chen, Fu
Effects of aflatoxin B(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks
title Effects of aflatoxin B(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks
title_full Effects of aflatoxin B(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks
title_fullStr Effects of aflatoxin B(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks
title_full_unstemmed Effects of aflatoxin B(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks
title_short Effects of aflatoxin B(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mRNA expression in ISA chicks
title_sort effects of aflatoxin b(1) on growth performance, antioxidant status, immune response, and pro-inflammatory cytokine mrna expression in isa chicks
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513573/
https://www.ncbi.nlm.nih.gov/pubmed/36176699
http://dx.doi.org/10.3389/fvets.2022.993039
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