Effects of Lateral Size, Thickness, and Stabilizer Concentration on the Cytotoxicity of Defect-Free Graphene Nanosheets: Implications for Biological Applications

[Image: see text] In this work, we apply liquid cascade centrifugation to highly concentrated graphene dispersions produced by liquid-phase exfoliation in water with an insoluble bis-pyrene stabilizer to obtain fractions containing nanosheets with different lateral size distributions. The concentrat...

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Autores principales: Hu, Chen-Xia, Read, Oliver, Shin, Yuyoung, Chen, Yingxian, Wang, Jingjing, Boyes, Matthew, Zeng, Niting, Panigrahi, Adyasha, Kostarelos, Kostas, Larrosa, Igor, Vranic, Sandra, Casiraghi, Cinzia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513747/
https://www.ncbi.nlm.nih.gov/pubmed/36185165
http://dx.doi.org/10.1021/acsanm.2c02403
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author Hu, Chen-Xia
Read, Oliver
Shin, Yuyoung
Chen, Yingxian
Wang, Jingjing
Boyes, Matthew
Zeng, Niting
Panigrahi, Adyasha
Kostarelos, Kostas
Larrosa, Igor
Vranic, Sandra
Casiraghi, Cinzia
author_facet Hu, Chen-Xia
Read, Oliver
Shin, Yuyoung
Chen, Yingxian
Wang, Jingjing
Boyes, Matthew
Zeng, Niting
Panigrahi, Adyasha
Kostarelos, Kostas
Larrosa, Igor
Vranic, Sandra
Casiraghi, Cinzia
author_sort Hu, Chen-Xia
collection PubMed
description [Image: see text] In this work, we apply liquid cascade centrifugation to highly concentrated graphene dispersions produced by liquid-phase exfoliation in water with an insoluble bis-pyrene stabilizer to obtain fractions containing nanosheets with different lateral size distributions. The concentration, stability, size, thickness, and the cytotoxicity profile are studied as a function of the initial stabilizer concentration for each fraction. Our results show that there is a critical initial amount of stabilizer (0.4 mg/mL) above which the dispersions show reduced concentration, stability, and biocompatibility, no matter the lateral size of the flakes.
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spelling pubmed-95137472022-09-28 Effects of Lateral Size, Thickness, and Stabilizer Concentration on the Cytotoxicity of Defect-Free Graphene Nanosheets: Implications for Biological Applications Hu, Chen-Xia Read, Oliver Shin, Yuyoung Chen, Yingxian Wang, Jingjing Boyes, Matthew Zeng, Niting Panigrahi, Adyasha Kostarelos, Kostas Larrosa, Igor Vranic, Sandra Casiraghi, Cinzia ACS Appl Nano Mater [Image: see text] In this work, we apply liquid cascade centrifugation to highly concentrated graphene dispersions produced by liquid-phase exfoliation in water with an insoluble bis-pyrene stabilizer to obtain fractions containing nanosheets with different lateral size distributions. The concentration, stability, size, thickness, and the cytotoxicity profile are studied as a function of the initial stabilizer concentration for each fraction. Our results show that there is a critical initial amount of stabilizer (0.4 mg/mL) above which the dispersions show reduced concentration, stability, and biocompatibility, no matter the lateral size of the flakes. American Chemical Society 2022-09-12 2022-09-23 /pmc/articles/PMC9513747/ /pubmed/36185165 http://dx.doi.org/10.1021/acsanm.2c02403 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Hu, Chen-Xia
Read, Oliver
Shin, Yuyoung
Chen, Yingxian
Wang, Jingjing
Boyes, Matthew
Zeng, Niting
Panigrahi, Adyasha
Kostarelos, Kostas
Larrosa, Igor
Vranic, Sandra
Casiraghi, Cinzia
Effects of Lateral Size, Thickness, and Stabilizer Concentration on the Cytotoxicity of Defect-Free Graphene Nanosheets: Implications for Biological Applications
title Effects of Lateral Size, Thickness, and Stabilizer Concentration on the Cytotoxicity of Defect-Free Graphene Nanosheets: Implications for Biological Applications
title_full Effects of Lateral Size, Thickness, and Stabilizer Concentration on the Cytotoxicity of Defect-Free Graphene Nanosheets: Implications for Biological Applications
title_fullStr Effects of Lateral Size, Thickness, and Stabilizer Concentration on the Cytotoxicity of Defect-Free Graphene Nanosheets: Implications for Biological Applications
title_full_unstemmed Effects of Lateral Size, Thickness, and Stabilizer Concentration on the Cytotoxicity of Defect-Free Graphene Nanosheets: Implications for Biological Applications
title_short Effects of Lateral Size, Thickness, and Stabilizer Concentration on the Cytotoxicity of Defect-Free Graphene Nanosheets: Implications for Biological Applications
title_sort effects of lateral size, thickness, and stabilizer concentration on the cytotoxicity of defect-free graphene nanosheets: implications for biological applications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9513747/
https://www.ncbi.nlm.nih.gov/pubmed/36185165
http://dx.doi.org/10.1021/acsanm.2c02403
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