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Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing

Fusarium ear rot (FER) caused by Fusarium verticillioides is a prevalent maize disease. To comprehensively characterize the genetic basis of the natural variation in FER resistance, a recombinant inbred line (RIL) population was used to map quantitative trait loci (QTL) for FER resistance. A total o...

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Autores principales: Xia, Yusheng, Wang, Baobao, Zhu, Lihong, Wu, Wenqi, Sun, Suli, Zhu, Zhendong, Li, Xinhai, Weng, Jianfeng, Duan, Canxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514021/
https://www.ncbi.nlm.nih.gov/pubmed/36176690
http://dx.doi.org/10.3389/fpls.2022.954546
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author Xia, Yusheng
Wang, Baobao
Zhu, Lihong
Wu, Wenqi
Sun, Suli
Zhu, Zhendong
Li, Xinhai
Weng, Jianfeng
Duan, Canxing
author_facet Xia, Yusheng
Wang, Baobao
Zhu, Lihong
Wu, Wenqi
Sun, Suli
Zhu, Zhendong
Li, Xinhai
Weng, Jianfeng
Duan, Canxing
author_sort Xia, Yusheng
collection PubMed
description Fusarium ear rot (FER) caused by Fusarium verticillioides is a prevalent maize disease. To comprehensively characterize the genetic basis of the natural variation in FER resistance, a recombinant inbred line (RIL) population was used to map quantitative trait loci (QTL) for FER resistance. A total of 17 QTL were identified by linkage mapping in eight environments. These QTL were located on six chromosomes and explained 3.88–15.62% of the total phenotypic variation. Moreover, qFER1.03 had the strongest effect and accounted for 4.98–15.62% of the phenotypic variation according to analyses of multiple environments involving best linear unbiased predictions. The chromosome segment substitution lines (CSSLs) derived from a cross between Qi319 (donor parent) and Ye478 (recurrent parent) were used to verify the contribution of qFER1.03 to FER resistance. The line CL171, which harbored an introgressed qFER1.03, was significantly resistant to FER. Further fine mapping of qFER1.03 revealed that the resistance QTL was linked to insertion/deletion markers InDel 8 and InDel 2, with physical distances of 43.55 Mb and 43.76 Mb, respectively. Additionally, qFER1.03 differed from the previous resistance QTL on chromosome 1. There were three annotated genes in this region. On the basis of the RNA-seq data, which revealed the genes differentially expressed between the FER-resistant Qi319 and susceptible Ye478, GRMZM2G017792 (MPK3) was preliminarily identified as a candidate gene in the qFER1.03 region. The Pr-CMV-VIGS system was used to decrease the GRMZM2G017792 expression level in CL171 by 34–57%, which led to a significant decrease in FER resistance. Using RIL and CSSL populations combined with RNA-seq and Pr-CMV-VIGS, the candidate gene can be dissected effectively, which provided important gene resource for breeding FER-resistant varieties.
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spelling pubmed-95140212022-09-28 Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing Xia, Yusheng Wang, Baobao Zhu, Lihong Wu, Wenqi Sun, Suli Zhu, Zhendong Li, Xinhai Weng, Jianfeng Duan, Canxing Front Plant Sci Plant Science Fusarium ear rot (FER) caused by Fusarium verticillioides is a prevalent maize disease. To comprehensively characterize the genetic basis of the natural variation in FER resistance, a recombinant inbred line (RIL) population was used to map quantitative trait loci (QTL) for FER resistance. A total of 17 QTL were identified by linkage mapping in eight environments. These QTL were located on six chromosomes and explained 3.88–15.62% of the total phenotypic variation. Moreover, qFER1.03 had the strongest effect and accounted for 4.98–15.62% of the phenotypic variation according to analyses of multiple environments involving best linear unbiased predictions. The chromosome segment substitution lines (CSSLs) derived from a cross between Qi319 (donor parent) and Ye478 (recurrent parent) were used to verify the contribution of qFER1.03 to FER resistance. The line CL171, which harbored an introgressed qFER1.03, was significantly resistant to FER. Further fine mapping of qFER1.03 revealed that the resistance QTL was linked to insertion/deletion markers InDel 8 and InDel 2, with physical distances of 43.55 Mb and 43.76 Mb, respectively. Additionally, qFER1.03 differed from the previous resistance QTL on chromosome 1. There were three annotated genes in this region. On the basis of the RNA-seq data, which revealed the genes differentially expressed between the FER-resistant Qi319 and susceptible Ye478, GRMZM2G017792 (MPK3) was preliminarily identified as a candidate gene in the qFER1.03 region. The Pr-CMV-VIGS system was used to decrease the GRMZM2G017792 expression level in CL171 by 34–57%, which led to a significant decrease in FER resistance. Using RIL and CSSL populations combined with RNA-seq and Pr-CMV-VIGS, the candidate gene can be dissected effectively, which provided important gene resource for breeding FER-resistant varieties. Frontiers Media S.A. 2022-09-13 /pmc/articles/PMC9514021/ /pubmed/36176690 http://dx.doi.org/10.3389/fpls.2022.954546 Text en Copyright © 2022 Xia, Wang, Zhu, Wu, Sun, Zhu, Li, Weng and Duan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xia, Yusheng
Wang, Baobao
Zhu, Lihong
Wu, Wenqi
Sun, Suli
Zhu, Zhendong
Li, Xinhai
Weng, Jianfeng
Duan, Canxing
Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing
title Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing
title_full Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing
title_fullStr Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing
title_full_unstemmed Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing
title_short Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing
title_sort identification of a fusarium ear rot resistance gene in maize by qtl mapping and rna sequencing
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514021/
https://www.ncbi.nlm.nih.gov/pubmed/36176690
http://dx.doi.org/10.3389/fpls.2022.954546
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