Cargando…
Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing
Fusarium ear rot (FER) caused by Fusarium verticillioides is a prevalent maize disease. To comprehensively characterize the genetic basis of the natural variation in FER resistance, a recombinant inbred line (RIL) population was used to map quantitative trait loci (QTL) for FER resistance. A total o...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514021/ https://www.ncbi.nlm.nih.gov/pubmed/36176690 http://dx.doi.org/10.3389/fpls.2022.954546 |
_version_ | 1784798188431998976 |
---|---|
author | Xia, Yusheng Wang, Baobao Zhu, Lihong Wu, Wenqi Sun, Suli Zhu, Zhendong Li, Xinhai Weng, Jianfeng Duan, Canxing |
author_facet | Xia, Yusheng Wang, Baobao Zhu, Lihong Wu, Wenqi Sun, Suli Zhu, Zhendong Li, Xinhai Weng, Jianfeng Duan, Canxing |
author_sort | Xia, Yusheng |
collection | PubMed |
description | Fusarium ear rot (FER) caused by Fusarium verticillioides is a prevalent maize disease. To comprehensively characterize the genetic basis of the natural variation in FER resistance, a recombinant inbred line (RIL) population was used to map quantitative trait loci (QTL) for FER resistance. A total of 17 QTL were identified by linkage mapping in eight environments. These QTL were located on six chromosomes and explained 3.88–15.62% of the total phenotypic variation. Moreover, qFER1.03 had the strongest effect and accounted for 4.98–15.62% of the phenotypic variation according to analyses of multiple environments involving best linear unbiased predictions. The chromosome segment substitution lines (CSSLs) derived from a cross between Qi319 (donor parent) and Ye478 (recurrent parent) were used to verify the contribution of qFER1.03 to FER resistance. The line CL171, which harbored an introgressed qFER1.03, was significantly resistant to FER. Further fine mapping of qFER1.03 revealed that the resistance QTL was linked to insertion/deletion markers InDel 8 and InDel 2, with physical distances of 43.55 Mb and 43.76 Mb, respectively. Additionally, qFER1.03 differed from the previous resistance QTL on chromosome 1. There were three annotated genes in this region. On the basis of the RNA-seq data, which revealed the genes differentially expressed between the FER-resistant Qi319 and susceptible Ye478, GRMZM2G017792 (MPK3) was preliminarily identified as a candidate gene in the qFER1.03 region. The Pr-CMV-VIGS system was used to decrease the GRMZM2G017792 expression level in CL171 by 34–57%, which led to a significant decrease in FER resistance. Using RIL and CSSL populations combined with RNA-seq and Pr-CMV-VIGS, the candidate gene can be dissected effectively, which provided important gene resource for breeding FER-resistant varieties. |
format | Online Article Text |
id | pubmed-9514021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95140212022-09-28 Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing Xia, Yusheng Wang, Baobao Zhu, Lihong Wu, Wenqi Sun, Suli Zhu, Zhendong Li, Xinhai Weng, Jianfeng Duan, Canxing Front Plant Sci Plant Science Fusarium ear rot (FER) caused by Fusarium verticillioides is a prevalent maize disease. To comprehensively characterize the genetic basis of the natural variation in FER resistance, a recombinant inbred line (RIL) population was used to map quantitative trait loci (QTL) for FER resistance. A total of 17 QTL were identified by linkage mapping in eight environments. These QTL were located on six chromosomes and explained 3.88–15.62% of the total phenotypic variation. Moreover, qFER1.03 had the strongest effect and accounted for 4.98–15.62% of the phenotypic variation according to analyses of multiple environments involving best linear unbiased predictions. The chromosome segment substitution lines (CSSLs) derived from a cross between Qi319 (donor parent) and Ye478 (recurrent parent) were used to verify the contribution of qFER1.03 to FER resistance. The line CL171, which harbored an introgressed qFER1.03, was significantly resistant to FER. Further fine mapping of qFER1.03 revealed that the resistance QTL was linked to insertion/deletion markers InDel 8 and InDel 2, with physical distances of 43.55 Mb and 43.76 Mb, respectively. Additionally, qFER1.03 differed from the previous resistance QTL on chromosome 1. There were three annotated genes in this region. On the basis of the RNA-seq data, which revealed the genes differentially expressed between the FER-resistant Qi319 and susceptible Ye478, GRMZM2G017792 (MPK3) was preliminarily identified as a candidate gene in the qFER1.03 region. The Pr-CMV-VIGS system was used to decrease the GRMZM2G017792 expression level in CL171 by 34–57%, which led to a significant decrease in FER resistance. Using RIL and CSSL populations combined with RNA-seq and Pr-CMV-VIGS, the candidate gene can be dissected effectively, which provided important gene resource for breeding FER-resistant varieties. Frontiers Media S.A. 2022-09-13 /pmc/articles/PMC9514021/ /pubmed/36176690 http://dx.doi.org/10.3389/fpls.2022.954546 Text en Copyright © 2022 Xia, Wang, Zhu, Wu, Sun, Zhu, Li, Weng and Duan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Xia, Yusheng Wang, Baobao Zhu, Lihong Wu, Wenqi Sun, Suli Zhu, Zhendong Li, Xinhai Weng, Jianfeng Duan, Canxing Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing |
title | Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing |
title_full | Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing |
title_fullStr | Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing |
title_full_unstemmed | Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing |
title_short | Identification of a Fusarium ear rot resistance gene in maize by QTL mapping and RNA sequencing |
title_sort | identification of a fusarium ear rot resistance gene in maize by qtl mapping and rna sequencing |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514021/ https://www.ncbi.nlm.nih.gov/pubmed/36176690 http://dx.doi.org/10.3389/fpls.2022.954546 |
work_keys_str_mv | AT xiayusheng identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing AT wangbaobao identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing AT zhulihong identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing AT wuwenqi identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing AT sunsuli identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing AT zhuzhendong identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing AT lixinhai identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing AT wengjianfeng identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing AT duancanxing identificationofafusariumearrotresistancegeneinmaizebyqtlmappingandrnasequencing |