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Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults
OBJECTIVE: The aim of this study was to determine the influence of polymorphisms in some key gene actors of the vitamin D (vitD) metabolic pathway on supplementation efficacy. METHODS: In total, 245 healthy participants were recruited from occupational medicine service in Sahloul University Hospital...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514197/ https://www.ncbi.nlm.nih.gov/pubmed/36167979 http://dx.doi.org/10.1038/s41430-022-01218-y |
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author | Ammar, Mariem Heni, Syrine Tira, Mohamed Sahbi Khalij, Yassine Hamdouni, Haithem Amor, Dorra Ksibi, Sonia Omezzine, Asma Bouslama, Ali |
author_facet | Ammar, Mariem Heni, Syrine Tira, Mohamed Sahbi Khalij, Yassine Hamdouni, Haithem Amor, Dorra Ksibi, Sonia Omezzine, Asma Bouslama, Ali |
author_sort | Ammar, Mariem |
collection | PubMed |
description | OBJECTIVE: The aim of this study was to determine the influence of polymorphisms in some key gene actors of the vitamin D (vitD) metabolic pathway on supplementation efficacy. METHODS: In total, 245 healthy participants were recruited from occupational medicine service in Sahloul University Hospital with vitD deficiency [25(OH)D ≤ 30 ng/ml]. After giving an informed consent, all participants were asked to complete a generalized questionnaire and to follow a detailed personalized supplementation protocol. Genetic study was performed by PCR-RFLP for 15 single nucleotide polymorphisms (SNPs) belonging to DBP, CYP2R1, CYP27B14, CYP24A1 and VDR genes. Statistical study was carried out with SPSS23.0. RESULTS: Among the studied SNPs, non-response was significantly associated with variant alleles of rs4588 (OR* = 11.51; p < 0.001), rs10766197 (OR* = 6.92; p = 0.008) and rs12794714 (OR* = 5.09; p = 0.004). These three SNPs contributed in 18.8% in response variability with rs4588 being the most influential (10.3%). There was a significant linear negative correlation between baseline 25(OH)D and post supplementation 25(OH)D concentration (r = −0.437; p < 0.001) as well as a linear negative association between the increase in 25(OH)D concentration and GRS (GRS: genetic risk score = the sum of risk alleles) (r = −0.149; p = 0.033). CONCLUSIONS: DBP-rs4588, CYP2R1-rs10766197 and rs12794714 variants are associated with variations in serum 25(OH)D concentrations and efficacy of response to vitD supplementation in Tunisian adults. Taking into account these variations can help to better adapt vitD intake to ensure a higher response to supplementation. |
format | Online Article Text |
id | pubmed-9514197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-95141972022-09-28 Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults Ammar, Mariem Heni, Syrine Tira, Mohamed Sahbi Khalij, Yassine Hamdouni, Haithem Amor, Dorra Ksibi, Sonia Omezzine, Asma Bouslama, Ali Eur J Clin Nutr Article OBJECTIVE: The aim of this study was to determine the influence of polymorphisms in some key gene actors of the vitamin D (vitD) metabolic pathway on supplementation efficacy. METHODS: In total, 245 healthy participants were recruited from occupational medicine service in Sahloul University Hospital with vitD deficiency [25(OH)D ≤ 30 ng/ml]. After giving an informed consent, all participants were asked to complete a generalized questionnaire and to follow a detailed personalized supplementation protocol. Genetic study was performed by PCR-RFLP for 15 single nucleotide polymorphisms (SNPs) belonging to DBP, CYP2R1, CYP27B14, CYP24A1 and VDR genes. Statistical study was carried out with SPSS23.0. RESULTS: Among the studied SNPs, non-response was significantly associated with variant alleles of rs4588 (OR* = 11.51; p < 0.001), rs10766197 (OR* = 6.92; p = 0.008) and rs12794714 (OR* = 5.09; p = 0.004). These three SNPs contributed in 18.8% in response variability with rs4588 being the most influential (10.3%). There was a significant linear negative correlation between baseline 25(OH)D and post supplementation 25(OH)D concentration (r = −0.437; p < 0.001) as well as a linear negative association between the increase in 25(OH)D concentration and GRS (GRS: genetic risk score = the sum of risk alleles) (r = −0.149; p = 0.033). CONCLUSIONS: DBP-rs4588, CYP2R1-rs10766197 and rs12794714 variants are associated with variations in serum 25(OH)D concentrations and efficacy of response to vitD supplementation in Tunisian adults. Taking into account these variations can help to better adapt vitD intake to ensure a higher response to supplementation. Nature Publishing Group UK 2022-09-27 2023 /pmc/articles/PMC9514197/ /pubmed/36167979 http://dx.doi.org/10.1038/s41430-022-01218-y Text en © The Author(s), under exclusive licence to Springer Nature Limited 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Ammar, Mariem Heni, Syrine Tira, Mohamed Sahbi Khalij, Yassine Hamdouni, Haithem Amor, Dorra Ksibi, Sonia Omezzine, Asma Bouslama, Ali Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults |
title | Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults |
title_full | Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults |
title_fullStr | Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults |
title_full_unstemmed | Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults |
title_short | Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults |
title_sort | variability in response to vitamin d supplementation according to vitamin d metabolism related gene polymorphisms in healthy adults |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514197/ https://www.ncbi.nlm.nih.gov/pubmed/36167979 http://dx.doi.org/10.1038/s41430-022-01218-y |
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