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Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults

OBJECTIVE: The aim of this study was to determine the influence of polymorphisms in some key gene actors of the vitamin D (vitD) metabolic pathway on supplementation efficacy. METHODS: In total, 245 healthy participants were recruited from occupational medicine service in Sahloul University Hospital...

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Autores principales: Ammar, Mariem, Heni, Syrine, Tira, Mohamed Sahbi, Khalij, Yassine, Hamdouni, Haithem, Amor, Dorra, Ksibi, Sonia, Omezzine, Asma, Bouslama, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514197/
https://www.ncbi.nlm.nih.gov/pubmed/36167979
http://dx.doi.org/10.1038/s41430-022-01218-y
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author Ammar, Mariem
Heni, Syrine
Tira, Mohamed Sahbi
Khalij, Yassine
Hamdouni, Haithem
Amor, Dorra
Ksibi, Sonia
Omezzine, Asma
Bouslama, Ali
author_facet Ammar, Mariem
Heni, Syrine
Tira, Mohamed Sahbi
Khalij, Yassine
Hamdouni, Haithem
Amor, Dorra
Ksibi, Sonia
Omezzine, Asma
Bouslama, Ali
author_sort Ammar, Mariem
collection PubMed
description OBJECTIVE: The aim of this study was to determine the influence of polymorphisms in some key gene actors of the vitamin D (vitD) metabolic pathway on supplementation efficacy. METHODS: In total, 245 healthy participants were recruited from occupational medicine service in Sahloul University Hospital with vitD deficiency [25(OH)D ≤ 30 ng/ml]. After giving an informed consent, all participants were asked to complete a generalized questionnaire and to follow a detailed personalized supplementation protocol. Genetic study was performed by PCR-RFLP for 15 single nucleotide polymorphisms (SNPs) belonging to DBP, CYP2R1, CYP27B14, CYP24A1 and VDR genes. Statistical study was carried out with SPSS23.0. RESULTS: Among the studied SNPs, non-response was significantly associated with variant alleles of rs4588 (OR* = 11.51; p < 0.001), rs10766197 (OR* = 6.92; p = 0.008) and rs12794714 (OR* = 5.09; p = 0.004). These three SNPs contributed in 18.8% in response variability with rs4588 being the most influential (10.3%). There was a significant linear negative correlation between baseline 25(OH)D and post supplementation 25(OH)D concentration (r = −0.437; p < 0.001) as well as a linear negative association between the increase in 25(OH)D concentration and GRS (GRS: genetic risk score = the sum of risk alleles) (r = −0.149; p = 0.033). CONCLUSIONS: DBP-rs4588, CYP2R1-rs10766197 and rs12794714 variants are associated with variations in serum 25(OH)D concentrations and efficacy of response to vitD supplementation in Tunisian adults. Taking into account these variations can help to better adapt vitD intake to ensure a higher response to supplementation.
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spelling pubmed-95141972022-09-28 Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults Ammar, Mariem Heni, Syrine Tira, Mohamed Sahbi Khalij, Yassine Hamdouni, Haithem Amor, Dorra Ksibi, Sonia Omezzine, Asma Bouslama, Ali Eur J Clin Nutr Article OBJECTIVE: The aim of this study was to determine the influence of polymorphisms in some key gene actors of the vitamin D (vitD) metabolic pathway on supplementation efficacy. METHODS: In total, 245 healthy participants were recruited from occupational medicine service in Sahloul University Hospital with vitD deficiency [25(OH)D ≤ 30 ng/ml]. After giving an informed consent, all participants were asked to complete a generalized questionnaire and to follow a detailed personalized supplementation protocol. Genetic study was performed by PCR-RFLP for 15 single nucleotide polymorphisms (SNPs) belonging to DBP, CYP2R1, CYP27B14, CYP24A1 and VDR genes. Statistical study was carried out with SPSS23.0. RESULTS: Among the studied SNPs, non-response was significantly associated with variant alleles of rs4588 (OR* = 11.51; p < 0.001), rs10766197 (OR* = 6.92; p = 0.008) and rs12794714 (OR* = 5.09; p = 0.004). These three SNPs contributed in 18.8% in response variability with rs4588 being the most influential (10.3%). There was a significant linear negative correlation between baseline 25(OH)D and post supplementation 25(OH)D concentration (r = −0.437; p < 0.001) as well as a linear negative association between the increase in 25(OH)D concentration and GRS (GRS: genetic risk score = the sum of risk alleles) (r = −0.149; p = 0.033). CONCLUSIONS: DBP-rs4588, CYP2R1-rs10766197 and rs12794714 variants are associated with variations in serum 25(OH)D concentrations and efficacy of response to vitD supplementation in Tunisian adults. Taking into account these variations can help to better adapt vitD intake to ensure a higher response to supplementation. Nature Publishing Group UK 2022-09-27 2023 /pmc/articles/PMC9514197/ /pubmed/36167979 http://dx.doi.org/10.1038/s41430-022-01218-y Text en © The Author(s), under exclusive licence to Springer Nature Limited 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Ammar, Mariem
Heni, Syrine
Tira, Mohamed Sahbi
Khalij, Yassine
Hamdouni, Haithem
Amor, Dorra
Ksibi, Sonia
Omezzine, Asma
Bouslama, Ali
Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults
title Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults
title_full Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults
title_fullStr Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults
title_full_unstemmed Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults
title_short Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults
title_sort variability in response to vitamin d supplementation according to vitamin d metabolism related gene polymorphisms in healthy adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514197/
https://www.ncbi.nlm.nih.gov/pubmed/36167979
http://dx.doi.org/10.1038/s41430-022-01218-y
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