One-pot synthesis of robust dendritic sulfur quantum dots for two-photon fluorescence imaging and “off–on” detection of hydroxyl radicals and ascorbic acid

The straightforward preparation of fluorescent sulfur quantum dots (SQDs) with good photostability and biocompatibility and multifunction remains a challenge. Herein, a simple method to improve the performance of SQDs is reported, that is, using hyperbranched polyglycerol (HPG) as a ligand to direct the synthesis of dendritic HPG-SQD nanocomposites from cheap elemental sulfur. Thanks to the protection of HPG, the HPG-SQDs show much better biocompatibility and photostability as compared with the widely reported polyethylene glycol (PEG) ligand-capped SQDs (PEG-SQDs). In addition, the HPG-SQDs also present excellent aqueous solubility, stable fluorescence against environmental variation, good cell uptake capability, and strong single- and two-photon fluorescence. Moreover, the HPG-SQDs display sensitive and selective fluorescence “off–on” behavior to hydroxyl radicals (˙OH) and ascorbic acid (AA), respectively, and thereby hold potential as a fluorescent switch to detect ˙OH and AA. For the first time, the utilization of two-photon fluorescence of HPG-SQDs to monitor ˙OH and AA in cells is demonstrated in this study.