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Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples
BACKGROUND: As new SARS-CoV-2 variants of concern emerge, there is a need to scale up testing to minimize transmission of the Coronavirus disease 2019 (COVID-19). Many countries especially those in the developing world continue to struggle with scaling up reverse transcriptase polymerase reaction (R...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514621/ https://www.ncbi.nlm.nih.gov/pubmed/36166414 http://dx.doi.org/10.1371/journal.pone.0269329 |
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author | Konstantinus, Iyaloo Chiwara, Douglas Ndevaetela, Emmy-Else Ndarukwa-Phiri, Victoria Garus-oas, Nathalia! Frans, Ndahafa Ndumbu, Pentikainen Shiningavamwe, Andreas van Rooyen, Gerhard Schiceya, Ferlin Hlahla, Lindile Namundjebo, Pendapala Ndozi-Okia, Ifeoma Chikuse, Francis Bantiewalu, Sirak Hailu Tjombonde, Kapena |
author_facet | Konstantinus, Iyaloo Chiwara, Douglas Ndevaetela, Emmy-Else Ndarukwa-Phiri, Victoria Garus-oas, Nathalia! Frans, Ndahafa Ndumbu, Pentikainen Shiningavamwe, Andreas van Rooyen, Gerhard Schiceya, Ferlin Hlahla, Lindile Namundjebo, Pendapala Ndozi-Okia, Ifeoma Chikuse, Francis Bantiewalu, Sirak Hailu Tjombonde, Kapena |
author_sort | Konstantinus, Iyaloo |
collection | PubMed |
description | BACKGROUND: As new SARS-CoV-2 variants of concern emerge, there is a need to scale up testing to minimize transmission of the Coronavirus disease 2019 (COVID-19). Many countries especially those in the developing world continue to struggle with scaling up reverse transcriptase polymerase reaction (RT-PCR) to detect SARS-CoV-2 due to scarcity of resources. Alternatives such as antigen rapid diagnostics tests (Ag-RDTs) may provide a solution to enable countries scale up testing. METHODS: In this study, we evaluated the Panbio(™) and STANDARD Q Ag-RDTs in the laboratory using 80 COVID-19 RT-PCR confirmed and 80 negative nasopharyngeal swabs. The STANDARD Q was further evaluated in the field on 112 symptomatic and 61 asymptomatic participants. RESULTS: For the laboratory evaluation, both tests had a sensitivity above 80% (Panbio(™) = 86% vs STANDARD Q = 88%). The specificity of the Panbio(™) was 100%, while that of the STANDARD Q was 99%. When evaluated in the field, the STANDARD Q maintained a high specificity of 99%, however the sensitivity was reduced to 56%. CONCLUSION: Using Ag-RDTs in low resource settings will be helpful in scaling-up SARS-CoV-2 testing, however, negative results should be confirmed by RT-PCR where possible to rule out COVID-19 infection. |
format | Online Article Text |
id | pubmed-9514621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-95146212022-09-28 Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples Konstantinus, Iyaloo Chiwara, Douglas Ndevaetela, Emmy-Else Ndarukwa-Phiri, Victoria Garus-oas, Nathalia! Frans, Ndahafa Ndumbu, Pentikainen Shiningavamwe, Andreas van Rooyen, Gerhard Schiceya, Ferlin Hlahla, Lindile Namundjebo, Pendapala Ndozi-Okia, Ifeoma Chikuse, Francis Bantiewalu, Sirak Hailu Tjombonde, Kapena PLoS One Research Article BACKGROUND: As new SARS-CoV-2 variants of concern emerge, there is a need to scale up testing to minimize transmission of the Coronavirus disease 2019 (COVID-19). Many countries especially those in the developing world continue to struggle with scaling up reverse transcriptase polymerase reaction (RT-PCR) to detect SARS-CoV-2 due to scarcity of resources. Alternatives such as antigen rapid diagnostics tests (Ag-RDTs) may provide a solution to enable countries scale up testing. METHODS: In this study, we evaluated the Panbio(™) and STANDARD Q Ag-RDTs in the laboratory using 80 COVID-19 RT-PCR confirmed and 80 negative nasopharyngeal swabs. The STANDARD Q was further evaluated in the field on 112 symptomatic and 61 asymptomatic participants. RESULTS: For the laboratory evaluation, both tests had a sensitivity above 80% (Panbio(™) = 86% vs STANDARD Q = 88%). The specificity of the Panbio(™) was 100%, while that of the STANDARD Q was 99%. When evaluated in the field, the STANDARD Q maintained a high specificity of 99%, however the sensitivity was reduced to 56%. CONCLUSION: Using Ag-RDTs in low resource settings will be helpful in scaling-up SARS-CoV-2 testing, however, negative results should be confirmed by RT-PCR where possible to rule out COVID-19 infection. Public Library of Science 2022-09-27 /pmc/articles/PMC9514621/ /pubmed/36166414 http://dx.doi.org/10.1371/journal.pone.0269329 Text en © 2022 Konstantinus et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Konstantinus, Iyaloo Chiwara, Douglas Ndevaetela, Emmy-Else Ndarukwa-Phiri, Victoria Garus-oas, Nathalia! Frans, Ndahafa Ndumbu, Pentikainen Shiningavamwe, Andreas van Rooyen, Gerhard Schiceya, Ferlin Hlahla, Lindile Namundjebo, Pendapala Ndozi-Okia, Ifeoma Chikuse, Francis Bantiewalu, Sirak Hailu Tjombonde, Kapena Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples |
title | Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples |
title_full | Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples |
title_fullStr | Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples |
title_full_unstemmed | Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples |
title_short | Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples |
title_sort | laboratory and field evaluation of the standard q and panbio(™) sars-cov-2 antigen rapid test in namibia using nasopharyngeal samples |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514621/ https://www.ncbi.nlm.nih.gov/pubmed/36166414 http://dx.doi.org/10.1371/journal.pone.0269329 |
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