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Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples

BACKGROUND: As new SARS-CoV-2 variants of concern emerge, there is a need to scale up testing to minimize transmission of the Coronavirus disease 2019 (COVID-19). Many countries especially those in the developing world continue to struggle with scaling up reverse transcriptase polymerase reaction (R...

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Autores principales: Konstantinus, Iyaloo, Chiwara, Douglas, Ndevaetela, Emmy-Else, Ndarukwa-Phiri, Victoria, Garus-oas, Nathalia!, Frans, Ndahafa, Ndumbu, Pentikainen, Shiningavamwe, Andreas, van Rooyen, Gerhard, Schiceya, Ferlin, Hlahla, Lindile, Namundjebo, Pendapala, Ndozi-Okia, Ifeoma, Chikuse, Francis, Bantiewalu, Sirak Hailu, Tjombonde, Kapena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514621/
https://www.ncbi.nlm.nih.gov/pubmed/36166414
http://dx.doi.org/10.1371/journal.pone.0269329
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author Konstantinus, Iyaloo
Chiwara, Douglas
Ndevaetela, Emmy-Else
Ndarukwa-Phiri, Victoria
Garus-oas, Nathalia!
Frans, Ndahafa
Ndumbu, Pentikainen
Shiningavamwe, Andreas
van Rooyen, Gerhard
Schiceya, Ferlin
Hlahla, Lindile
Namundjebo, Pendapala
Ndozi-Okia, Ifeoma
Chikuse, Francis
Bantiewalu, Sirak Hailu
Tjombonde, Kapena
author_facet Konstantinus, Iyaloo
Chiwara, Douglas
Ndevaetela, Emmy-Else
Ndarukwa-Phiri, Victoria
Garus-oas, Nathalia!
Frans, Ndahafa
Ndumbu, Pentikainen
Shiningavamwe, Andreas
van Rooyen, Gerhard
Schiceya, Ferlin
Hlahla, Lindile
Namundjebo, Pendapala
Ndozi-Okia, Ifeoma
Chikuse, Francis
Bantiewalu, Sirak Hailu
Tjombonde, Kapena
author_sort Konstantinus, Iyaloo
collection PubMed
description BACKGROUND: As new SARS-CoV-2 variants of concern emerge, there is a need to scale up testing to minimize transmission of the Coronavirus disease 2019 (COVID-19). Many countries especially those in the developing world continue to struggle with scaling up reverse transcriptase polymerase reaction (RT-PCR) to detect SARS-CoV-2 due to scarcity of resources. Alternatives such as antigen rapid diagnostics tests (Ag-RDTs) may provide a solution to enable countries scale up testing. METHODS: In this study, we evaluated the Panbio(™) and STANDARD Q Ag-RDTs in the laboratory using 80 COVID-19 RT-PCR confirmed and 80 negative nasopharyngeal swabs. The STANDARD Q was further evaluated in the field on 112 symptomatic and 61 asymptomatic participants. RESULTS: For the laboratory evaluation, both tests had a sensitivity above 80% (Panbio(™) = 86% vs STANDARD Q = 88%). The specificity of the Panbio(™) was 100%, while that of the STANDARD Q was 99%. When evaluated in the field, the STANDARD Q maintained a high specificity of 99%, however the sensitivity was reduced to 56%. CONCLUSION: Using Ag-RDTs in low resource settings will be helpful in scaling-up SARS-CoV-2 testing, however, negative results should be confirmed by RT-PCR where possible to rule out COVID-19 infection.
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spelling pubmed-95146212022-09-28 Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples Konstantinus, Iyaloo Chiwara, Douglas Ndevaetela, Emmy-Else Ndarukwa-Phiri, Victoria Garus-oas, Nathalia! Frans, Ndahafa Ndumbu, Pentikainen Shiningavamwe, Andreas van Rooyen, Gerhard Schiceya, Ferlin Hlahla, Lindile Namundjebo, Pendapala Ndozi-Okia, Ifeoma Chikuse, Francis Bantiewalu, Sirak Hailu Tjombonde, Kapena PLoS One Research Article BACKGROUND: As new SARS-CoV-2 variants of concern emerge, there is a need to scale up testing to minimize transmission of the Coronavirus disease 2019 (COVID-19). Many countries especially those in the developing world continue to struggle with scaling up reverse transcriptase polymerase reaction (RT-PCR) to detect SARS-CoV-2 due to scarcity of resources. Alternatives such as antigen rapid diagnostics tests (Ag-RDTs) may provide a solution to enable countries scale up testing. METHODS: In this study, we evaluated the Panbio(™) and STANDARD Q Ag-RDTs in the laboratory using 80 COVID-19 RT-PCR confirmed and 80 negative nasopharyngeal swabs. The STANDARD Q was further evaluated in the field on 112 symptomatic and 61 asymptomatic participants. RESULTS: For the laboratory evaluation, both tests had a sensitivity above 80% (Panbio(™) = 86% vs STANDARD Q = 88%). The specificity of the Panbio(™) was 100%, while that of the STANDARD Q was 99%. When evaluated in the field, the STANDARD Q maintained a high specificity of 99%, however the sensitivity was reduced to 56%. CONCLUSION: Using Ag-RDTs in low resource settings will be helpful in scaling-up SARS-CoV-2 testing, however, negative results should be confirmed by RT-PCR where possible to rule out COVID-19 infection. Public Library of Science 2022-09-27 /pmc/articles/PMC9514621/ /pubmed/36166414 http://dx.doi.org/10.1371/journal.pone.0269329 Text en © 2022 Konstantinus et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Konstantinus, Iyaloo
Chiwara, Douglas
Ndevaetela, Emmy-Else
Ndarukwa-Phiri, Victoria
Garus-oas, Nathalia!
Frans, Ndahafa
Ndumbu, Pentikainen
Shiningavamwe, Andreas
van Rooyen, Gerhard
Schiceya, Ferlin
Hlahla, Lindile
Namundjebo, Pendapala
Ndozi-Okia, Ifeoma
Chikuse, Francis
Bantiewalu, Sirak Hailu
Tjombonde, Kapena
Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples
title Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples
title_full Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples
title_fullStr Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples
title_full_unstemmed Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples
title_short Laboratory and field evaluation of the STANDARD Q and Panbio(™) SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samples
title_sort laboratory and field evaluation of the standard q and panbio(™) sars-cov-2 antigen rapid test in namibia using nasopharyngeal samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514621/
https://www.ncbi.nlm.nih.gov/pubmed/36166414
http://dx.doi.org/10.1371/journal.pone.0269329
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