Cargando…

Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory

The spider mite Tetranychus urticae is an economically important agricultural pest, which feeds on a broad spectrum of plant species. In an RNAseq experiment performed in our laboratory, 4 of the 15 members of the RIN4-like/NOI family of Arabidopsis thaliana were significantly overexpressed after T....

Descripción completa

Detalles Bibliográficos
Autores principales: Contreras, Estefania, Martinez, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514647/
https://www.ncbi.nlm.nih.gov/pubmed/36166429
http://dx.doi.org/10.1371/journal.pone.0270791
_version_ 1784798323095371776
author Contreras, Estefania
Martinez, Manuel
author_facet Contreras, Estefania
Martinez, Manuel
author_sort Contreras, Estefania
collection PubMed
description The spider mite Tetranychus urticae is an economically important agricultural pest, which feeds on a broad spectrum of plant species. In an RNAseq experiment performed in our laboratory, 4 of the 15 members of the RIN4-like/NOI family of Arabidopsis thaliana were significantly overexpressed after T. urticae infestation. Two of them (NOI3 and NOI5) are shorter and harbour one NOI domain, which characterises this family, and the other two (NOI10 and NOI11) have two-NOI domains. The only member of this family characterized is RIN4, a two-NOI intrinsically disordered protein anchored to the plasma membrane and involved in plant defence against bacterial pathogens. The function of all other members of the RIN4-like/NOI Arabidopsis family and their putative role in herbivore defence remains unknown. We perform a comparative genomic analysis of RIN4-like/NOI sequences to study the evolutionary features of this protein family and the distribution of its members among species. We show that short one-NOI proteins were more numerous and exhibited lower disorder propensity compared to two-NOI members. NOI10 and NOI11, from the two-NOI group, are included in a clade-specific expansion of Brassicaceae with unique predicted posttranslational modification sites and clear predicted structural differences from RIN4. Our analysis suggests that the members of the RIN4-like/NOI family upregulated after mite feeding have novel functions different from those assigned to RIN4, likely involving adaptation to stress specialisation.
format Online
Article
Text
id pubmed-9514647
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-95146472022-09-28 Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory Contreras, Estefania Martinez, Manuel PLoS One Research Article The spider mite Tetranychus urticae is an economically important agricultural pest, which feeds on a broad spectrum of plant species. In an RNAseq experiment performed in our laboratory, 4 of the 15 members of the RIN4-like/NOI family of Arabidopsis thaliana were significantly overexpressed after T. urticae infestation. Two of them (NOI3 and NOI5) are shorter and harbour one NOI domain, which characterises this family, and the other two (NOI10 and NOI11) have two-NOI domains. The only member of this family characterized is RIN4, a two-NOI intrinsically disordered protein anchored to the plasma membrane and involved in plant defence against bacterial pathogens. The function of all other members of the RIN4-like/NOI Arabidopsis family and their putative role in herbivore defence remains unknown. We perform a comparative genomic analysis of RIN4-like/NOI sequences to study the evolutionary features of this protein family and the distribution of its members among species. We show that short one-NOI proteins were more numerous and exhibited lower disorder propensity compared to two-NOI members. NOI10 and NOI11, from the two-NOI group, are included in a clade-specific expansion of Brassicaceae with unique predicted posttranslational modification sites and clear predicted structural differences from RIN4. Our analysis suggests that the members of the RIN4-like/NOI family upregulated after mite feeding have novel functions different from those assigned to RIN4, likely involving adaptation to stress specialisation. Public Library of Science 2022-09-27 /pmc/articles/PMC9514647/ /pubmed/36166429 http://dx.doi.org/10.1371/journal.pone.0270791 Text en © 2022 Contreras, Martinez https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Contreras, Estefania
Martinez, Manuel
Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory
title Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory
title_full Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory
title_fullStr Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory
title_full_unstemmed Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory
title_short Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory
title_sort comparative and evolutionary analysis of arabidopsis rin4-like/noi proteins induced by herbivory
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514647/
https://www.ncbi.nlm.nih.gov/pubmed/36166429
http://dx.doi.org/10.1371/journal.pone.0270791
work_keys_str_mv AT contrerasestefania comparativeandevolutionaryanalysisofarabidopsisrin4likenoiproteinsinducedbyherbivory
AT martinezmanuel comparativeandevolutionaryanalysisofarabidopsisrin4likenoiproteinsinducedbyherbivory