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Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory
The spider mite Tetranychus urticae is an economically important agricultural pest, which feeds on a broad spectrum of plant species. In an RNAseq experiment performed in our laboratory, 4 of the 15 members of the RIN4-like/NOI family of Arabidopsis thaliana were significantly overexpressed after T....
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514647/ https://www.ncbi.nlm.nih.gov/pubmed/36166429 http://dx.doi.org/10.1371/journal.pone.0270791 |
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author | Contreras, Estefania Martinez, Manuel |
author_facet | Contreras, Estefania Martinez, Manuel |
author_sort | Contreras, Estefania |
collection | PubMed |
description | The spider mite Tetranychus urticae is an economically important agricultural pest, which feeds on a broad spectrum of plant species. In an RNAseq experiment performed in our laboratory, 4 of the 15 members of the RIN4-like/NOI family of Arabidopsis thaliana were significantly overexpressed after T. urticae infestation. Two of them (NOI3 and NOI5) are shorter and harbour one NOI domain, which characterises this family, and the other two (NOI10 and NOI11) have two-NOI domains. The only member of this family characterized is RIN4, a two-NOI intrinsically disordered protein anchored to the plasma membrane and involved in plant defence against bacterial pathogens. The function of all other members of the RIN4-like/NOI Arabidopsis family and their putative role in herbivore defence remains unknown. We perform a comparative genomic analysis of RIN4-like/NOI sequences to study the evolutionary features of this protein family and the distribution of its members among species. We show that short one-NOI proteins were more numerous and exhibited lower disorder propensity compared to two-NOI members. NOI10 and NOI11, from the two-NOI group, are included in a clade-specific expansion of Brassicaceae with unique predicted posttranslational modification sites and clear predicted structural differences from RIN4. Our analysis suggests that the members of the RIN4-like/NOI family upregulated after mite feeding have novel functions different from those assigned to RIN4, likely involving adaptation to stress specialisation. |
format | Online Article Text |
id | pubmed-9514647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-95146472022-09-28 Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory Contreras, Estefania Martinez, Manuel PLoS One Research Article The spider mite Tetranychus urticae is an economically important agricultural pest, which feeds on a broad spectrum of plant species. In an RNAseq experiment performed in our laboratory, 4 of the 15 members of the RIN4-like/NOI family of Arabidopsis thaliana were significantly overexpressed after T. urticae infestation. Two of them (NOI3 and NOI5) are shorter and harbour one NOI domain, which characterises this family, and the other two (NOI10 and NOI11) have two-NOI domains. The only member of this family characterized is RIN4, a two-NOI intrinsically disordered protein anchored to the plasma membrane and involved in plant defence against bacterial pathogens. The function of all other members of the RIN4-like/NOI Arabidopsis family and their putative role in herbivore defence remains unknown. We perform a comparative genomic analysis of RIN4-like/NOI sequences to study the evolutionary features of this protein family and the distribution of its members among species. We show that short one-NOI proteins were more numerous and exhibited lower disorder propensity compared to two-NOI members. NOI10 and NOI11, from the two-NOI group, are included in a clade-specific expansion of Brassicaceae with unique predicted posttranslational modification sites and clear predicted structural differences from RIN4. Our analysis suggests that the members of the RIN4-like/NOI family upregulated after mite feeding have novel functions different from those assigned to RIN4, likely involving adaptation to stress specialisation. Public Library of Science 2022-09-27 /pmc/articles/PMC9514647/ /pubmed/36166429 http://dx.doi.org/10.1371/journal.pone.0270791 Text en © 2022 Contreras, Martinez https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Contreras, Estefania Martinez, Manuel Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory |
title | Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory |
title_full | Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory |
title_fullStr | Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory |
title_full_unstemmed | Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory |
title_short | Comparative and evolutionary analysis of Arabidopsis RIN4-like/NOI proteins induced by herbivory |
title_sort | comparative and evolutionary analysis of arabidopsis rin4-like/noi proteins induced by herbivory |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514647/ https://www.ncbi.nlm.nih.gov/pubmed/36166429 http://dx.doi.org/10.1371/journal.pone.0270791 |
work_keys_str_mv | AT contrerasestefania comparativeandevolutionaryanalysisofarabidopsisrin4likenoiproteinsinducedbyherbivory AT martinezmanuel comparativeandevolutionaryanalysisofarabidopsisrin4likenoiproteinsinducedbyherbivory |