Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities

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Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-C...

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Autores principales: Li, Lin, Uppal, Timsy, Hartley, Paul D., Gorzalski, Andrew, Pandori, Mark, Picker, Michael A., Verma, Subhash C., Pagilla, Krishna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514676/
https://www.ncbi.nlm.nih.gov/pubmed/36167869
http://dx.doi.org/10.1038/s41598-022-20219-2
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author Li, Lin
Uppal, Timsy
Hartley, Paul D.
Gorzalski, Andrew
Pandori, Mark
Picker, Michael A.
Verma, Subhash C.
Pagilla, Krishna
author_facet Li, Lin
Uppal, Timsy
Hartley, Paul D.
Gorzalski, Andrew
Pandori, Mark
Picker, Michael A.
Verma, Subhash C.
Pagilla, Krishna
author_sort Li, Lin
collection PubMed
description Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-CoV-2 variants, including those being monitored for having enhanced transmissibility. We sequenced genomic RNA derived from wastewater to determine the variants of coronaviruses circulating in the communities. Wastewater samples were collected from Truckee Meadows Water Reclamation Facility (TMWRF) from November 2020 to June 2021. SARS-CoV-2 variants resulting from wastewater were compared with the variants detected in infected individuals' clinical specimens (nasal/nasopharyngeal swabs) during the same period and found conclusively in agreement. Therefore, wastewater monitoring for SARS-CoV-2 variants in the community is a feasible strategy as a complementary tool to clinical specimen testing in the latter's absence.
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spelling pubmed-95146762022-09-28 Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities Li, Lin Uppal, Timsy Hartley, Paul D. Gorzalski, Andrew Pandori, Mark Picker, Michael A. Verma, Subhash C. Pagilla, Krishna Sci Rep Article Detection of SARS-CoV-2 viral load in wastewater has been highly informative in estimating the approximate number of infected individuals in the surrounding communities. Recent developments in wastewater monitoring to determine community prevalence of COVID-19 further extends into identifying SARS-CoV-2 variants, including those being monitored for having enhanced transmissibility. We sequenced genomic RNA derived from wastewater to determine the variants of coronaviruses circulating in the communities. Wastewater samples were collected from Truckee Meadows Water Reclamation Facility (TMWRF) from November 2020 to June 2021. SARS-CoV-2 variants resulting from wastewater were compared with the variants detected in infected individuals' clinical specimens (nasal/nasopharyngeal swabs) during the same period and found conclusively in agreement. Therefore, wastewater monitoring for SARS-CoV-2 variants in the community is a feasible strategy as a complementary tool to clinical specimen testing in the latter's absence. Nature Publishing Group UK 2022-09-27 /pmc/articles/PMC9514676/ /pubmed/36167869 http://dx.doi.org/10.1038/s41598-022-20219-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Li, Lin
Uppal, Timsy
Hartley, Paul D.
Gorzalski, Andrew
Pandori, Mark
Picker, Michael A.
Verma, Subhash C.
Pagilla, Krishna
Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities
title Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities
title_full Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities
title_fullStr Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities
title_full_unstemmed Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities
title_short Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities
title_sort detecting sars-cov-2 variants in wastewater and their correlation with circulating variants in the communities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9514676/
https://www.ncbi.nlm.nih.gov/pubmed/36167869
http://dx.doi.org/10.1038/s41598-022-20219-2
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