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P216 Rare isolates from subcutaneous mycotic lesions; A study from tertiary care center in Chhattisgarh, India

POSTER SESSION 2, SEPTEMBER 22, 2022, 12:30 PM - 1:30 PM:   AIMS AND OBJECTIVE: To identify the causative agents of suspected subcutaneous mycosis patients attending to a tertiary care hospital, Chhattisgarh, India. INTRODUCTION: Subcutaneous mycoses are a group of fungal infections of dermis and su...

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Autores principales: Keche, Archana, Tigga, Richa, Satyaki, Ganguly, Chhabra, Namrata, Gupta, Rakesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9515939/
http://dx.doi.org/10.1093/mmy/myac072.P216
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author Keche, Archana
Tigga, Richa
Satyaki, Ganguly
Chhabra, Namrata
Gupta, Rakesh
author_facet Keche, Archana
Tigga, Richa
Satyaki, Ganguly
Chhabra, Namrata
Gupta, Rakesh
author_sort Keche, Archana
collection PubMed
description POSTER SESSION 2, SEPTEMBER 22, 2022, 12:30 PM - 1:30 PM:   AIMS AND OBJECTIVE: To identify the causative agents of suspected subcutaneous mycosis patients attending to a tertiary care hospital, Chhattisgarh, India. INTRODUCTION: Subcutaneous mycoses are a group of fungal infections of dermis and subcutaneous tissue caused by both melanized and hyaline molds. It often affects patients with immunosuppressive conditions. It consists of Sporotrichosis, Chromoblastomycosis, Phaeohyphomycosis, Hyalohyphomycosis, Mycetoma, subcutaneous zygomycosis, Rhinosporidiosis, Lobomycosis, and disseminated Penicilliosis. There are proven pathogenic agents causing subcutaneous mycosis though are not regularly isolated and reported. Few of them are commonly come across in the laboratory. Herewith, emphasized on the unusual clinical isolates from the patients having subcutaneous mycotic lesions with their clinical details. METHOD: It is a retrospective descriptive analysis of data of subcutaneous mycosis cases of duration January 2019 to March 2022. Total 52 clinical specimens from the suspected subcutaneous mycotic lesion were studied. Male dominance was observed amongst the patients. Amongst 52, 31% were detected positive for fungal elements by direct microscopy in 20% KOH mount, 55.7%, 25% positivity was observed in Culture and by both KOH wet mount and culture. Samples were processed and identified by using standard protocol. The significant unusual isolates identified were Conidiobolus coronatus from subcutaneous cyst from buttocks, Medicopsis romerai from fine needle aspiration from the nodule of left thumb, Rhytidhysteron rufulum from right lateral malleolus, Aquastroma magniostiolata species from subcutaneous cyst on lateral aspect of left lower leg above lateral malleolus, Aspergillus tamorii, Aspergillus glaucus, Chetomium species, Aspergillus montevidensis, Cladosporium sphaerospermum. Phenotypically unidentified isolates were sent to NCCPF PGI, Chandigarh for final identification. CONCLUSION: There is diversity in the etiological agents of subcutaneous mycoses. Every case is different and rare. With the help of molecular techniques, it became possible to identify unusual fungal isolates from subcutaneous infection. Awareness and extensive studies are required to evidence the pathogenicity and associated complication due to these fungal infections. It will also help to regulate the therapeutic management and to know the geographical distribution of unusual fungal agents.
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spelling pubmed-95159392022-09-28 P216 Rare isolates from subcutaneous mycotic lesions; A study from tertiary care center in Chhattisgarh, India Keche, Archana Tigga, Richa Satyaki, Ganguly Chhabra, Namrata Gupta, Rakesh Med Mycol Oral Presentations POSTER SESSION 2, SEPTEMBER 22, 2022, 12:30 PM - 1:30 PM:   AIMS AND OBJECTIVE: To identify the causative agents of suspected subcutaneous mycosis patients attending to a tertiary care hospital, Chhattisgarh, India. INTRODUCTION: Subcutaneous mycoses are a group of fungal infections of dermis and subcutaneous tissue caused by both melanized and hyaline molds. It often affects patients with immunosuppressive conditions. It consists of Sporotrichosis, Chromoblastomycosis, Phaeohyphomycosis, Hyalohyphomycosis, Mycetoma, subcutaneous zygomycosis, Rhinosporidiosis, Lobomycosis, and disseminated Penicilliosis. There are proven pathogenic agents causing subcutaneous mycosis though are not regularly isolated and reported. Few of them are commonly come across in the laboratory. Herewith, emphasized on the unusual clinical isolates from the patients having subcutaneous mycotic lesions with their clinical details. METHOD: It is a retrospective descriptive analysis of data of subcutaneous mycosis cases of duration January 2019 to March 2022. Total 52 clinical specimens from the suspected subcutaneous mycotic lesion were studied. Male dominance was observed amongst the patients. Amongst 52, 31% were detected positive for fungal elements by direct microscopy in 20% KOH mount, 55.7%, 25% positivity was observed in Culture and by both KOH wet mount and culture. Samples were processed and identified by using standard protocol. The significant unusual isolates identified were Conidiobolus coronatus from subcutaneous cyst from buttocks, Medicopsis romerai from fine needle aspiration from the nodule of left thumb, Rhytidhysteron rufulum from right lateral malleolus, Aquastroma magniostiolata species from subcutaneous cyst on lateral aspect of left lower leg above lateral malleolus, Aspergillus tamorii, Aspergillus glaucus, Chetomium species, Aspergillus montevidensis, Cladosporium sphaerospermum. Phenotypically unidentified isolates were sent to NCCPF PGI, Chandigarh for final identification. CONCLUSION: There is diversity in the etiological agents of subcutaneous mycoses. Every case is different and rare. With the help of molecular techniques, it became possible to identify unusual fungal isolates from subcutaneous infection. Awareness and extensive studies are required to evidence the pathogenicity and associated complication due to these fungal infections. It will also help to regulate the therapeutic management and to know the geographical distribution of unusual fungal agents. Oxford University Press 2022-09-20 /pmc/articles/PMC9515939/ http://dx.doi.org/10.1093/mmy/myac072.P216 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Oral Presentations
Keche, Archana
Tigga, Richa
Satyaki, Ganguly
Chhabra, Namrata
Gupta, Rakesh
P216 Rare isolates from subcutaneous mycotic lesions; A study from tertiary care center in Chhattisgarh, India
title P216 Rare isolates from subcutaneous mycotic lesions; A study from tertiary care center in Chhattisgarh, India
title_full P216 Rare isolates from subcutaneous mycotic lesions; A study from tertiary care center in Chhattisgarh, India
title_fullStr P216 Rare isolates from subcutaneous mycotic lesions; A study from tertiary care center in Chhattisgarh, India
title_full_unstemmed P216 Rare isolates from subcutaneous mycotic lesions; A study from tertiary care center in Chhattisgarh, India
title_short P216 Rare isolates from subcutaneous mycotic lesions; A study from tertiary care center in Chhattisgarh, India
title_sort p216 rare isolates from subcutaneous mycotic lesions; a study from tertiary care center in chhattisgarh, india
topic Oral Presentations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9515939/
http://dx.doi.org/10.1093/mmy/myac072.P216
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