Cargando…

Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis

Fragaria viridis exhibits S-RNase-based gametophytic self-incompatibility, in which S-RNase is the major factor inhibiting pollen tube growth. However, the pathways involved in and the immediate causes of the inhibition of pollen tube growth remain unknown. Here, interactive RNA sequencing and prote...

Descripción completa

Detalles Bibliográficos
Autores principales: Du, Jianke, Ge, Chunfeng, Wang, Tao, Wang, Jing, Ni, Zhiyou, Xiao, Shiwei, Zhao, Fengli, Zhao, Mizhen, Qiao, Yushan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9515988/
https://www.ncbi.nlm.nih.gov/pubmed/36186066
http://dx.doi.org/10.3389/fpls.2022.927001
_version_ 1784798614290169856
author Du, Jianke
Ge, Chunfeng
Wang, Tao
Wang, Jing
Ni, Zhiyou
Xiao, Shiwei
Zhao, Fengli
Zhao, Mizhen
Qiao, Yushan
author_facet Du, Jianke
Ge, Chunfeng
Wang, Tao
Wang, Jing
Ni, Zhiyou
Xiao, Shiwei
Zhao, Fengli
Zhao, Mizhen
Qiao, Yushan
author_sort Du, Jianke
collection PubMed
description Fragaria viridis exhibits S-RNase-based gametophytic self-incompatibility, in which S-RNase is the major factor inhibiting pollen tube growth. However, the pathways involved in and the immediate causes of the inhibition of pollen tube growth remain unknown. Here, interactive RNA sequencing and proteome analysis revealed changes in the transcriptomic and proteomic profiles of F. viridis styles harvested at 0 and 24 h after self-pollination. A total of 2,181 differentially expressed genes and 200 differentially abundant proteins were identified during the pollen development stage of self-pollination. Differentially expressed genes and differentially abundant proteins associated with self-incompatible pollination were further mined, and multiple pathways were found to be involved. Interestingly, the expression pattern of the transcription factor FviYABBY1, which is linked to polar growth, differed from those of other genes within the same family. Specifically, FviYABBY1 expression was extremely high in pollen, and its expression trend in self-pollinated styles was consistent with that of S-RNase. Furthermore, FviYABBY1 interacted with S-RNase in a non-S haplotype way. Therefore, FviYABBY1 affects the expression of polar growth-related genes in self-pollen tubes and is positively regulated by S-RNase.
format Online
Article
Text
id pubmed-9515988
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95159882022-09-29 Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis Du, Jianke Ge, Chunfeng Wang, Tao Wang, Jing Ni, Zhiyou Xiao, Shiwei Zhao, Fengli Zhao, Mizhen Qiao, Yushan Front Plant Sci Plant Science Fragaria viridis exhibits S-RNase-based gametophytic self-incompatibility, in which S-RNase is the major factor inhibiting pollen tube growth. However, the pathways involved in and the immediate causes of the inhibition of pollen tube growth remain unknown. Here, interactive RNA sequencing and proteome analysis revealed changes in the transcriptomic and proteomic profiles of F. viridis styles harvested at 0 and 24 h after self-pollination. A total of 2,181 differentially expressed genes and 200 differentially abundant proteins were identified during the pollen development stage of self-pollination. Differentially expressed genes and differentially abundant proteins associated with self-incompatible pollination were further mined, and multiple pathways were found to be involved. Interestingly, the expression pattern of the transcription factor FviYABBY1, which is linked to polar growth, differed from those of other genes within the same family. Specifically, FviYABBY1 expression was extremely high in pollen, and its expression trend in self-pollinated styles was consistent with that of S-RNase. Furthermore, FviYABBY1 interacted with S-RNase in a non-S haplotype way. Therefore, FviYABBY1 affects the expression of polar growth-related genes in self-pollen tubes and is positively regulated by S-RNase. Frontiers Media S.A. 2022-09-14 /pmc/articles/PMC9515988/ /pubmed/36186066 http://dx.doi.org/10.3389/fpls.2022.927001 Text en Copyright © 2022 Du, Ge, Wang, Wang, Ni, Xiao, Zhao, Zhao and Qiao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Du, Jianke
Ge, Chunfeng
Wang, Tao
Wang, Jing
Ni, Zhiyou
Xiao, Shiwei
Zhao, Fengli
Zhao, Mizhen
Qiao, Yushan
Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis
title Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis
title_full Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis
title_fullStr Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis
title_full_unstemmed Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis
title_short Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis
title_sort combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of fviyabby1 in self-incompatibility in fragaria viridis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9515988/
https://www.ncbi.nlm.nih.gov/pubmed/36186066
http://dx.doi.org/10.3389/fpls.2022.927001
work_keys_str_mv AT dujianke combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis
AT gechunfeng combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis
AT wangtao combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis
AT wangjing combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis
AT nizhiyou combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis
AT xiaoshiwei combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis
AT zhaofengli combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis
AT zhaomizhen combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis
AT qiaoyushan combinedtranscriptomicandproteomicanalysisrevealsmultiplepathwaysinvolvedinselfpollentubedevelopmentandthepotentialrolesoffviyabby1inselfincompatibilityinfragariaviridis